Sistemik Fare Kandidoz Modelinde Antifungal Kombinasyonlarının Etkinliği

Cem Artan; Nedret Koç; Müge Oğuzkaya Artan

doi:10.5174/tutfd.2009.02272.1

The Effect of Combinations of Antimycotics on Systemic Candidiasis in a Mouse Model

Year 2010, Volume: 2010 Issue: 5, 334 - 337, 01.05.2010

Cem Artan Nedret Koç Müge Oğuzkaya Artan

https://doi.org/10.5174/tutfd.2009.02272.1

Abstract

Objective: The purpose of this study was to test the hypothesis thThis study was planned to investigate the antipneumocystis activity of terbinafine in a rat model. Material and Methods: Rats were obtained from the Hakan Çetinsaya Experimental and Clinical Research Institutions, Erciyes University, Kayseri, Turkey. Terbinafine administered orally in doses of 40, 80, 120 and 160 mg/kg/day after nine weeks of immunosuppression with dexamethasone to facilitate the development of acute Pneumocystis carinii penumoniae (PCP). Results: Untreated animals showed P. carinii infection levels with a mean (&plusmn;standard deviation) log number of cysts per gram of lung tissue of 4.6&plusmn;1.6 at the end of the experiment. Terbinafine administered at a dose of 160 mg/kg/day significantly reduced the log number of cysts per gram to 2.2&plusmn;1.5. The therapeutic efficacy of terbinafine administered at 160 mg/kg/day (log 2.2&plusmn;1.5 cysts/lung) was similar to that obtained with trimethoprim-sulfamethoxazole (TMP-SMX), 50/250 mg/kg/day (p&lt;0.001). A reduction in the number of cysts was also observed in infected animals treated with 80, and 120 mg of terbinafine/kg/day, although the results were not statistically significant (p&gt;0.05). Conclusion: In our model, the efficacy of terbinafine in PCP has been found to be dose dependent. Turkish Başlık: Sistemik Fare Kandidoz Modelinde Antifungal Kombinasyonlarının Etkinliği Anahtar Kelimeler: Kandidoz; fare; terbinafin; kombinasyon Amaç: Bu çalışma rat modelinde terbinafinin anti-Pneumocystis aktivitesini belirlemek için planlanmıştır. Gereç ve Yöntemler: Ratlar Erciyes Üniversitesi Deneysel ve Klinik Araştırma Merkezin&#39;den sağlanmıştır. Dokuz hafta boyunca akut Pneumocystis carinii pnömonisi (PCP) oluşturmak üzere, deksamethazon ile immünsüpresyon yapılan ratlara oral olarak 40, 80, 120 ve 160 mg/kg/gün terbinafin verildi. Bulgular: Çalışmanın sonunda tedavi almayan grupda PCP enfeksiyon düzeyi ortalama (&plusmn;standart sapma) akciğer dokusu gramındaki kist sayısı log 4.6&plusmn;1.6 idi. Terbinafin 160mg/kg/gün uygulanan grupta gramdaki kist sayısında önemli düşüş gözlendi log 2.2&plusmn;1.5. Terbinafin 160mg/kg/gün uygulanan tedavi etkinliği, trimetoprim-sulfametoksazol (TMP-SMX), 50/250 mg/kg/gün uygulaması ile benzer sonuç verdi (p&lt;0.001). Terbinafin 80 ve 120 mg/kg/gün uygulamalarında da kist sayısında düşüş görülmekle birlikte bu değerler istatistiksel olarak anlamlı değildi (p&gt;0.05). Sonuç: Bizim modelimizde terbinafinin PCP etkinliği doza bağlı bulundu.

Keywords

Candidiasis; combination; mice; terbinafine

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