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Antibiotic Susceptibilities and Serotyping of Clinical Streptococcus Agalactiae Isolates

Year 2011, Volume: 2011 Issue: 4, 362 - 365, 01.04.2011
https://doi.org/10.5174/tutfd.2010.03979.2

Abstract

Objective: Streptococcus agalactiae (Group B streptococci, GBS) are frequently responsible for sepsis and meningitis seen in the early weeks of life. GBS may cause perinatal infection and premature birth in pregnant women. The aim of this study was to serotype GBS strains isolated from clinical samples and evaluate their serotype distribution according to their susceptibilities to antibiotics and isolation sites. Material and Methods: One hundred thirty one S. agalactiae strains isolated from the clinical samples were included in the study. Of the strains, 99 were isolated from urine, 20 from soft tissue, 10 from blood and 2 from vaginal swab. Penicillin G and ceftriaxone susceptibilities of GBS were determined by the agar dilution method. Susceptibilities to erythromycin, clindamycin, vancomycin and tetracycline were determined by the Kirby-Bauer method according to CLSI criteria. Serotyping was performed using the latex aglutination method using specific antisera (Ia, Ib, II-VIII). Results: While in 131 GBS strains, serotypes VII and VIII were not detected, the most frequently isolated serotypes were types Ia (36%), III (30.5%) and II (13%) respectively. Serotype Ia was the most frequently seen serotype in all samples. All GBS isolates were susceptible to penicilin G, ceftriaxone and vancomycin. Among the strains, tetracycline, erythromycin and clindamycin resistance rates were determined as 90%, 14.5%, and 13% respectively. Conclusion: Penicillin is still the first choice of treatment for the infections with all serotypes of S. agalactiae in Turkey.

References

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  • Flugge K, Supper S, Siedler A, Berner R. Serotype distribution of invasive group B streptococcal isolates in infants: results from a nationwide active laboratory surveillance study over 2 years in Germany. Clin Infect Dis 2005;40:760-3. [CrossRef]
  • Matsubara K, Nishiyama Y, Katayama K, Yamamoto G, Sugiyama M, Murai T, et al. Change of antimicrobial susceptibility of group B solated cci over 15 years in Japan. J Antimicrob Chemother 2001;48: 579-82. [CrossRef]
  • Terakubo S, Ichiman Y, Takemura H, Yamamoto H, Shimada J, Nakashima H. Serotypes and antibody levels of group B strepto- cocci in pregnant women. Kansenshogaku Zasshi 2003;77:121-6.
  • Zeng X, Kong F, Morgan J, Gilbert GL. Evaluation of a multiplex PCR-based reverse line blot-hybridization assay for identification of serotype and surface protein antigens of Streptococcus aga- lactiae. J Clin Microbiol 2006;44:3822-5. [CrossRef] Balkan Med J 2011; 28: 362-5

Antibiotic Susceptibilities and Serotyping of Clinical Streptococcus Agalactiae Isolates

Year 2011, Volume: 2011 Issue: 4, 362 - 365, 01.04.2011
https://doi.org/10.5174/tutfd.2010.03979.2

Abstract

References

  • Schuchat A. Group B streptococcus. Lancet 1999;353:51-6. [CrossRef]
  • Regan JA, Klebanoff MA, Nugent RP, Eschenbach DA, Black- welder WC, Lou Y, et al. Colonization with group B streptococci in pregnancy and adverse outcome. VIP Study Group. Am J Ob- stet Gynecol 1996;174:1354-60. [CrossRef]
  • Farley MM. Group B streptococcal disease in non pregnant adults. Clin Infect Dis 2001;33:556-61. [CrossRef]
  • Clinical and Laboratory Standards Institute. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobi- cally; Approved Standard- Eighth Edition. CLSI document M07- A8. Clinical and Laboratory Standards Institute, Wayne, Pennsyl- vania, 2009.
  • Clinical and Laboratory Standards Institute. Performance stan- dards for Antimicrobial Susceptibility Testing; Nineteenth Infor- mational Supplement. CLSI document M100-S19. Clinical and Laboratory Standards Institute, Wayne, Pennsylvania, 2009.
  • Seppala H, Nissinen A, Yu Q, Huovinen P. Three different pheno- types of erythromycin-resistant Streptococcus pyogenes in Fin- land. J Antimicrob Chemother 1993;32:885-91. [CrossRef]
  • Başustaoğlu A. Klinik Mikrobiyoloji (Manual of Clinical Microbiol- ogy). 9.baskı.Ankara, Atlas Yayınları,2009:415.
  • Campbell JR, Hillier SL, Krohn MA, Ferrieri P, Zaleznik DF, et al. Group B streptococcal colonization and serotype-specific immunity in pregnant women at delivery. Obstet Gynecol 2000;96:498-503. [CrossRef]
  • Flugge K, Supper S, Siedler A, Berner R. Serotype distribution of invasive group B streptococcal isolates in infants: results from a nationwide active laboratory surveillance study over 2 years in Germany. Clin Infect Dis 2005;40:760-3. [CrossRef]
  • Matsubara K, Nishiyama Y, Katayama K, Yamamoto G, Sugiyama M, Murai T, et al. Change of antimicrobial susceptibility of group B solated cci over 15 years in Japan. J Antimicrob Chemother 2001;48: 579-82. [CrossRef]
  • Terakubo S, Ichiman Y, Takemura H, Yamamoto H, Shimada J, Nakashima H. Serotypes and antibody levels of group B strepto- cocci in pregnant women. Kansenshogaku Zasshi 2003;77:121-6.
  • Zeng X, Kong F, Morgan J, Gilbert GL. Evaluation of a multiplex PCR-based reverse line blot-hybridization assay for identification of serotype and surface protein antigens of Streptococcus aga- lactiae. J Clin Microbiol 2006;44:3822-5. [CrossRef] Balkan Med J 2011; 28: 362-5
There are 12 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Articles
Authors

Altay Atalay This is me

Mehmet Ölçü This is me

Duygu Perçin This is me

Publication Date April 1, 2011
Published in Issue Year 2011 Volume: 2011 Issue: 4

Cite

APA Atalay, A., Ölçü, M., & Perçin, D. (2011). Antibiotic Susceptibilities and Serotyping of Clinical Streptococcus Agalactiae Isolates. Balkan Medical Journal, 2011(4), 362-365. https://doi.org/10.5174/tutfd.2010.03979.2
AMA Atalay A, Ölçü M, Perçin D. Antibiotic Susceptibilities and Serotyping of Clinical Streptococcus Agalactiae Isolates. Balkan Medical Journal. April 2011;2011(4):362-365. doi:10.5174/tutfd.2010.03979.2
Chicago Atalay, Altay, Mehmet Ölçü, and Duygu Perçin. “Antibiotic Susceptibilities and Serotyping of Clinical Streptococcus Agalactiae Isolates”. Balkan Medical Journal 2011, no. 4 (April 2011): 362-65. https://doi.org/10.5174/tutfd.2010.03979.2.
EndNote Atalay A, Ölçü M, Perçin D (April 1, 2011) Antibiotic Susceptibilities and Serotyping of Clinical Streptococcus Agalactiae Isolates. Balkan Medical Journal 2011 4 362–365.
IEEE A. Atalay, M. Ölçü, and D. Perçin, “Antibiotic Susceptibilities and Serotyping of Clinical Streptococcus Agalactiae Isolates”, Balkan Medical Journal, vol. 2011, no. 4, pp. 362–365, 2011, doi: 10.5174/tutfd.2010.03979.2.
ISNAD Atalay, Altay et al. “Antibiotic Susceptibilities and Serotyping of Clinical Streptococcus Agalactiae Isolates”. Balkan Medical Journal 2011/4 (April 2011), 362-365. https://doi.org/10.5174/tutfd.2010.03979.2.
JAMA Atalay A, Ölçü M, Perçin D. Antibiotic Susceptibilities and Serotyping of Clinical Streptococcus Agalactiae Isolates. Balkan Medical Journal. 2011;2011:362–365.
MLA Atalay, Altay et al. “Antibiotic Susceptibilities and Serotyping of Clinical Streptococcus Agalactiae Isolates”. Balkan Medical Journal, vol. 2011, no. 4, 2011, pp. 362-5, doi:10.5174/tutfd.2010.03979.2.
Vancouver Atalay A, Ölçü M, Perçin D. Antibiotic Susceptibilities and Serotyping of Clinical Streptococcus Agalactiae Isolates. Balkan Medical Journal. 2011;2011(4):362-5.