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Surface Alteration in Aging of Rat Tubae Uterinae Epithelium

Year 2012, Volume: 2012 Issue: 4, 370 - 375, 01.04.2012

Abstract

Objective: Aim of the study was evaluating arrangement of apical surface differentiation in cross-sections of tuba uterinae in different age groups by scanning electron microscope. Material and Methods: Six groups were created with 36 Wistar rat; 1st group: neonate (1th day) (n=6), 2nd group: young (22nd day) (n=6), 3rd group: prepubertal (4-6 week) (n=6), 4th group: adult (10 week) (n=6), 5th group: premenopausal (8 month) (n=6), 6th group: old (18-20 month) (n=6). Tissue samples examined with scanning electron microscope. Results: When surface differentiations of epithelial cells in tubae uterinae from birth to menopause were considered, it was determined that the cell with microvilli are first maturing cells and degenerated by ages first. It was observed that the ciliated cells are last maturing cells and subsisting as a mature cell during the postmenopausal period. Conclusion: Towards the menopause degeneration in microvillous cells together with lack of secretion may affect sperm nutrition adversely. The increase of ciliated cells in aging may be a physiological result related to the active role of cilia movement in the sperm and early embryo transport against a probable decrease in muscle contraction in aging. Turkish Başlık: Yaşa Bağlı Sıçan Tuba Uterina Epitelinde Gözlenen Yüzey Değişiklikleri Anahtar Kelimeler: Tuba Uterina, epitel, yaşlanma, taramalı elektron mikroskop, rat Amaç: Çalışmamızda değişik yaş gruplarındaki tuba uterina kesitlerinde apikal yüzey farklanmalarının düzenleniminin tarayıcı elektron mikroskop ile değerlendirilmesi amaçlandı. Gereç ve Yöntem: Gazi Üniversitesi Deney Hayvanları Etik Kurulu tarafından onaylanan çalışmamızda 36 Wistar tipi sıçandan 6 grup oluşturuldu; 1. grup: yenidoğan (1. gün) (n=6), 2. grup: genç (22. gün) (n=6), 3. grup: prepubertal (4-6 hafta) (n=6), 4. grup: yetişkin (10 hafta) (n=6), 5. grup: premenapozal (8 ay) (n=6), 6. grup: yaşlı (18-20 ay) (n=6). doku örnekleri JEOL JSM-6060LV tarayıcı elektron mikraskopta incelendi. Bulgular: Doğum sonrasından menopoza kadar tuba uterina'daki epitel hücrelerin yüzey farklanmaları değerlendirildiğinde, mikrovilluslu hücrelerin ilk olarak olgunlaşan ve yaşa bağlı ilk dejenere olan hücre formu oldukları belirlendi. Silyalı hücrelerin son olgunlaşan hücreler oldukları ve post menopozal dönemde de gelişkin olarak varlıklarını sürdürdükleri izlendi. Sonuç: Sonuç olarak menopoza doğru ilerledikçe, mikrovilluslu hücrelerde görülen dejenerasyonla birlikte salgılama yetersizliğinin spermiyum beslenmesini olumsuz yönde etkiliyor olabileceği kanısına varıldı. Silyalı hücrelerde gözlenen yaşa bağlı artışın ise yaşa bağlı kas kontraksiyonunda olası azalmaya karşı silya hareketinin spermiyum ve erken embriyon transportunda aktif rol oynamasına bağlı gerçekleşen fizyolojik bir sonuç olabileceği kanısına varıldı.

References

  • Safwat MD, Habib FA, Oweiss NY. Distribution of macrophages in the human fallopian tubes: an immunohistochemical and electron microscopic study. Folia Morphol (Warsz) 2008;67:43-52.
  • Yaniz JL, Lopez-Gatius F, Hunter RH. Scanning electron micro- scopic study of the functional anatomy of the porcine oviductal mucosa. Anat Histol Embryol 2006;35:28-34. [CrossRef]
  • Umezu T, Tomooka Y. An evidence of stromal cell populations functionally linked with epithelial cell populations in the mouse oviduct. Zoolog Sci 2004;21:319-26. [CrossRef]
  • Crow J, Amso NN, Lewin J, Shaw RW. Morphology and ultra- structure of fallopian tube epithelium at different stages of the menstrual cycle and menopause. Hum Reprod 1994;9:2224-33.
  • Abe H, Onodera M, Sugawara S. Scanning electron microscopy of goat oviductal epithelial cells at the follicular and luteal phases of the oestrus cycle. J Anat 1993;183:415-21.
  • Abe H, Oikawa T. Regional differences in the ultrastructural fea- tures of secretory cells in the golden hamster (Mesocricetus aura- tus) oviductal epithelium. J Anat 1991;175:147-58.
  • Odor DL, Gaddum-Rosse P, Rumery RE. Secretory cells of the oviduct of the pig-tailed monkey, Macaca nemestrina, during the menstrual cycle and after estrogen treatment. Am J Anat 1983;166:149-72. [CrossRef]
  • Verhage HG, Mavrogianis PA, Boice ML, Li W, Fazleabas AT. Oviductal epithelium of the baboon: hormonal control and the immuno-gold localization of oviduct-specific glycoproteins. Am J Anat 1990;187:81-90. [CrossRef]
  • Abe H, Oikawa T. Observations by scanning electron microscopy of oviductal epithelial cells from cows at follicular and luteal phas- es. Anat Rec 1993;235:399-410. [CrossRef]
  • Abe H, Oikawa T. Examination by scanning electron micros- copy of oviductal epithelium of the prolific Chinese Meis- han pig at follicular and luteal phases. Anat Rec 1992;233: 399-408. [CrossRef]
  • Makabe S, Motta PM, Naguro T, Vizza E, Perrone G, Zichella L. Mi- croanatomy of the female reproductive organs in postmenopause by scanning electron microscopy. Climacteric 1998;1:63-71. [CrossRef]
  • Myers RK, Cook JE, Mosier JE. Comparative aging changes in canine uterine tubes (oviducts): electron microscopy. Am J Vet Res 1984;45:2008-14.
  • Correr S, Makabe S, Heyn R, Relucenti M, Naguro T, Familiari G. Microplicae-like structures of the fallopian tube in postmeno- pausal women as shown by electron microscopy. Histol Histo- pathol 2006;21:219-26.
  • Nayak RK, Albert EN, Kassira WN. Cyclic ultrastructural changes in ewe uterine tube (oviduct) infundibular epithelium. Am J Vet Res 1976;37:923-33.

Surface Alteration in Aging of Rat Tubae Uterinae Epithelium

Year 2012, Volume: 2012 Issue: 4, 370 - 375, 01.04.2012

Abstract

References

  • Safwat MD, Habib FA, Oweiss NY. Distribution of macrophages in the human fallopian tubes: an immunohistochemical and electron microscopic study. Folia Morphol (Warsz) 2008;67:43-52.
  • Yaniz JL, Lopez-Gatius F, Hunter RH. Scanning electron micro- scopic study of the functional anatomy of the porcine oviductal mucosa. Anat Histol Embryol 2006;35:28-34. [CrossRef]
  • Umezu T, Tomooka Y. An evidence of stromal cell populations functionally linked with epithelial cell populations in the mouse oviduct. Zoolog Sci 2004;21:319-26. [CrossRef]
  • Crow J, Amso NN, Lewin J, Shaw RW. Morphology and ultra- structure of fallopian tube epithelium at different stages of the menstrual cycle and menopause. Hum Reprod 1994;9:2224-33.
  • Abe H, Onodera M, Sugawara S. Scanning electron microscopy of goat oviductal epithelial cells at the follicular and luteal phases of the oestrus cycle. J Anat 1993;183:415-21.
  • Abe H, Oikawa T. Regional differences in the ultrastructural fea- tures of secretory cells in the golden hamster (Mesocricetus aura- tus) oviductal epithelium. J Anat 1991;175:147-58.
  • Odor DL, Gaddum-Rosse P, Rumery RE. Secretory cells of the oviduct of the pig-tailed monkey, Macaca nemestrina, during the menstrual cycle and after estrogen treatment. Am J Anat 1983;166:149-72. [CrossRef]
  • Verhage HG, Mavrogianis PA, Boice ML, Li W, Fazleabas AT. Oviductal epithelium of the baboon: hormonal control and the immuno-gold localization of oviduct-specific glycoproteins. Am J Anat 1990;187:81-90. [CrossRef]
  • Abe H, Oikawa T. Observations by scanning electron microscopy of oviductal epithelial cells from cows at follicular and luteal phas- es. Anat Rec 1993;235:399-410. [CrossRef]
  • Abe H, Oikawa T. Examination by scanning electron micros- copy of oviductal epithelium of the prolific Chinese Meis- han pig at follicular and luteal phases. Anat Rec 1992;233: 399-408. [CrossRef]
  • Makabe S, Motta PM, Naguro T, Vizza E, Perrone G, Zichella L. Mi- croanatomy of the female reproductive organs in postmenopause by scanning electron microscopy. Climacteric 1998;1:63-71. [CrossRef]
  • Myers RK, Cook JE, Mosier JE. Comparative aging changes in canine uterine tubes (oviducts): electron microscopy. Am J Vet Res 1984;45:2008-14.
  • Correr S, Makabe S, Heyn R, Relucenti M, Naguro T, Familiari G. Microplicae-like structures of the fallopian tube in postmeno- pausal women as shown by electron microscopy. Histol Histo- pathol 2006;21:219-26.
  • Nayak RK, Albert EN, Kassira WN. Cyclic ultrastructural changes in ewe uterine tube (oviduct) infundibular epithelium. Am J Vet Res 1976;37:923-33.
There are 14 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Articles
Authors

Şule Şahin This is me

Gülnur Take Kaplanoğlu This is me

Deniz Erdoğan This is me

Mahmud Bağırzade This is me

Zekiye Suludere This is me

Publication Date April 1, 2012
Published in Issue Year 2012 Volume: 2012 Issue: 4

Cite

APA Şahin, Ş., Kaplanoğlu, G. . T., Erdoğan, D., Bağırzade, M., et al. (2012). Surface Alteration in Aging of Rat Tubae Uterinae Epithelium. Balkan Medical Journal, 2012(4), 370-375.
AMA Şahin Ş, Kaplanoğlu GT, Erdoğan D, Bağırzade M, Suludere Z. Surface Alteration in Aging of Rat Tubae Uterinae Epithelium. Balkan Medical Journal. April 2012;2012(4):370-375.
Chicago Şahin, Şule, Gülnur Take Kaplanoğlu, Deniz Erdoğan, Mahmud Bağırzade, and Zekiye Suludere. “Surface Alteration in Aging of Rat Tubae Uterinae Epithelium”. Balkan Medical Journal 2012, no. 4 (April 2012): 370-75.
EndNote Şahin Ş, Kaplanoğlu GT, Erdoğan D, Bağırzade M, Suludere Z (April 1, 2012) Surface Alteration in Aging of Rat Tubae Uterinae Epithelium. Balkan Medical Journal 2012 4 370–375.
IEEE Ş. Şahin, G. . T. Kaplanoğlu, D. Erdoğan, M. Bağırzade, and Z. Suludere, “Surface Alteration in Aging of Rat Tubae Uterinae Epithelium”, Balkan Medical Journal, vol. 2012, no. 4, pp. 370–375, 2012.
ISNAD Şahin, Şule et al. “Surface Alteration in Aging of Rat Tubae Uterinae Epithelium”. Balkan Medical Journal 2012/4 (April 2012), 370-375.
JAMA Şahin Ş, Kaplanoğlu GT, Erdoğan D, Bağırzade M, Suludere Z. Surface Alteration in Aging of Rat Tubae Uterinae Epithelium. Balkan Medical Journal. 2012;2012:370–375.
MLA Şahin, Şule et al. “Surface Alteration in Aging of Rat Tubae Uterinae Epithelium”. Balkan Medical Journal, vol. 2012, no. 4, 2012, pp. 370-5.
Vancouver Şahin Ş, Kaplanoğlu GT, Erdoğan D, Bağırzade M, Suludere Z. Surface Alteration in Aging of Rat Tubae Uterinae Epithelium. Balkan Medical Journal. 2012;2012(4):370-5.