Comparison of Sirolimus and Colchicine Treatment on the Development of Peritoneal Fibrozis in Rats Having Peritoneal Dialysis

Year 2015, Volume: 32 Issue: 1, 101 - 106, 01.01.2015

Tamer Sağıroğlu Serhat Oğuz Mustafa Burak Sayhan Mehmet A. Yağcı Tülin Yalta Gönül Sağıroğlu Elif Çopuroğlu

Abstract

Background: Continuous ambulatory peritoneal dialysis
is a successful treatment modality for patients with
end-stage renal disease. Peritoneal fibrosis (PF) is the
most critical complication of long-term peritoneal dialysis
(PD).
Aims: In our study, we aimed to compare the effects of
colchicine and sirolimus on PF induced by hypertonic
peritoneal dialysis solutions in rats.
Study Design: Animal experiment.
Methods: Twenty-four rats were randomly divided
into three groups. The control group received an intraperitoneal
injection (ip) of saline. The sirolimus group
received the PD solution, plus 1.0 mg/kg/day Rapamune®.
The colchicine group received the PD solution
ip plus 1.0 mg/kg/day of colchicine. Blood samples
were taken to measure the serum levels of VEGF,
TGF-β, and TNF-α. Peritoneal tissue samples were
taken for histopathological evaluation.
Results: TGF-β and TNF-α values in the sirolimus group
were found to be statistically significantly lower than in
the control and colchicine groups, but the differences between
the control and colchicine groups were not statistically
significant. No statistically significant differences
were found between the groups regarding the VEGF values.
Vascular neogenesis and peritoneal thickness were
compared; the values in the sirolimus group were statistically
reduced compared to the values in the control group.
Mild fibrosis developed in 75% of all animals in the sirolimus
group; there was no moderate or severe fibrosis
observed. Fibrosis developed to varying degrees in 100%
of the animals in the control and colchicine groups.
Conclusion: The present study demonstrates that sirolimus
might be beneficial for preventing or delaying
the progression of PF and neoangiogenesis. These alterations
in the peritoneal membrane may be connected

Keywords

Sirolimus, kolşisin, peritoneal fibrozis, peritonal diyaliz

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