Detection of Human Bocavirus DNA by Multiplex PCR Analysis: Postmortem Case Report

Year 2015, Volume: 32 Issue: 2, 226 - 229, 01.04.2015

Nihan Ziyade Neval Elgörmüş Gözde Şirin Taner Daş

Abstract

Background: Human bocavirus (HBoV) is a virus
belonging to the Parvoviridae family, which has been
newly discovered to be associated with respiratory tract
infections in children. There are many reports worldwide
on the endemicity of this virus. Since it is relatively
new, it is not routinely detected in clinical laboratory
investigations.
Case Report: We demonstrated that HBoV infection
caused the death of a 5-month-old girl with a history
of high fever and wheezing. Human bocavirus (HBoV
1/2/3/4) was found in a nasopharyngeal swab, paraffin-embedded
lung tissue and stool samples by multiplex
PCR methods using postmortem microbiological
analysis.
Conclusion: This case suggests that lower respiratory
tract infections due to HBoV may cause severe and
life-threatening diseases. Postmortem microbiology
is useful in both clinical and forensic autopsies, and
allows a suspected infection to be confirmed. To our
knowledge, this report is the first document of a HBoV
postmortem case in Turkey

Keywords

Autopsy, forensic science, human bocavirus, multiplex PCR, postmortem microbiology, respiratory tract infection

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