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Year 2015, Volume: 32 Issue: 4, 388 - 396, 01.10.2015

Abstract

References

  • 1. Miettinen M, Lasota J. Gastrointestinal stromal tumors: review on morphology, molecular pathology, prognosis, and differential diagnosis [review]. Arch Pathol Lab Med 2006;130:1466-78.
  • 2. Miettinen M, Wang ZF, Lasota J. DOG1 antibody in the differential diagnosis of gastrointestinal stromal tumors: a study of 1840 cases. Am J Surg Pathol 2009;33:1401-8. [CrossRef]
  • 3. Espinosa I, Lee CH, Kim MK, Rouse BT, Subramanian S, Montgomery K, et al. A novel monoclonal antibody against DOG1 is a sensitive and specific marker for gastrointestinal stromal tumors. Am J Surg Pathol 2008;32:210-8. [CrossRef]
  • 4. West RB, Corless CL, Chen X, Rubin BP, Subramanian S, Montgomery K, et al. The novel marker, DOG1, is expressed ubiquitously in gastrointestinal stromal tumors ırrespective of KIT or PDGFRA mutation status. Am J Pathol 2004;165:107-13. [CrossRef]
  • 5. Ardeleanu C, Arsene D, Hinescu M, Andrei F, Gutu D, Luca L, et al. Pancreatic expression of DOG1 A novel gastrointestinal stromal tumor (GIST) biomarker. Appl Immunohistochem Mol Morphol 2009;17:413-8. [CrossRef]
  • 6. Liegl B, Hornick JL, Corless CL, Fletcher CD. Monoclonal antibody DOG1.1 shows higher sensitivity than KIT in the diagnosis of gastrointestinal stromal tumors, including unusual subtypes. Am J Surg Pathol 2009;33:437-46. [CrossRef]
  • 7. Lopes LF, West RB, Bacchi LM, van de Rijn M, Bacchi CE. DOG1 for the diagnosis of gastrointestinal stromal tumor (GIST): Comparison between 2 different antibodies. Appl Immunohistochem Mol Morphol 2010;18:333-7. [CrossRef]
  • 8. Novelli R, Rossi S, Rodriguez-Justo M, Taniere P, Seddon B, Toffolatti L, et al. DOG1and CD117 are the antibodies of choice in the diagnosis of gastrointestinal stromal tumours. Histopathology 2010;57:259-70. [CrossRef]
  • 9. Miwa S, Nakajima T, Murai Y, Takano Y, Sugiyama T. Mutation assay of the novel gene DOG1 in gastrointestinal stromal tumors (GISTs). J Gastroenterol 2008;43:531-7. [CrossRef]
  • 10. Lee CH, Liang CW, Espinosa I. The utility of discovered on gastrointestinal stromal tumor 1 (DOG1) antibody in surgical pathology-the GIST of it [review]. Adv Anat Pathol 2010;17:222- 32. [CrossRef]
  • 11. Demetri GD, von Mehren M, Antonescu CR, DeMatteo RP, Ganjoo KN, Maki RG, et al. NCCN Task Force Report: Update on the management of patients with Gastrointestinal Stromal Tumors. J Natl Compr Canc Netw 2010;8(Suppl 2):S1-41.
  • 12. Katoh M, Katoh M. FLJ10261 gene, located within the CCND1- EMS1 locus on human chromosome 11q13, encodes the eight-transmembrane protein homologous to C12orf3, C11orf25 and FLJ34272 gene products. Int J Oncol 2003;22:1375-81. [CrossRef]
  • 13. Galindo BE, Vacquier VD. Phylogeny of the TMEM16 protein family: some members are overexpressed in cancer. Int J Mol Med 2005;16:919-24. [CrossRef]
  • 14. Carles A, Millon R, Cromer A, Ganguli G, Lemaire F, Young J, et al. Head and neck squamous cell carcinoma transcriptome analysis by comprehensive validated differential display. Oncogene 2006;25:1821-31. [CrossRef]
  • 15. Wong NA. Gastrointestinal stromal tumours-an update for Histopathologists. Histopathology 2011;59:807-21. [CrossRef]
  • 16. Caputo A, Caci E, Ferrera L, Pedemonte N, Barsanti C, Sondo E, et al. TMEM16A, a membrane protein associated with calciumdependent chloride channel activity. Science 2008;322:590-4. [CrossRef]

Histopathological Features of Gastrointestinal Stromal Tumors and the Contribution of DOG1 Expression to the Diagnosis

Year 2015, Volume: 32 Issue: 4, 388 - 396, 01.10.2015

Abstract

Background: Gastrointestinal stromal tumors (GIST) have KIT or platelet-derived growth factor receptor α (PDGFRα) mutations affecting receptor tyrosine kinase activity and do not benefit from classic treatment regimens. Aims: The aim of this study was to review the algorithm that may be followed for the diagnosis and differential diagnosis in GISTs by investigating the histomorphological parameters and expression characteristics of classical immunohistochemical antibodies used in routine tests in addition to DOG1 expression. Study Design: Diagnostic accuracy study. Methods: We reevaluated the histological and immunohistochemical parameters of 37 GISTs. The standard immunohistochemical diagnosis and differential diagnosis panel antibodies (CD117, PDGFRα, CD34, vimentin, desmin, SMA, S-100, and Ki67) were studied on the tumor sections. We also used the popular marker DOG1 antibody with accepted sensitivity for GISTs in recent years and the PDGFRα immune marker for which the benefit in routine practice is discussed. Results: Classification according to progressive disease risk groups of the 37 cases revealed that 54% were in the high risk, 19% in the moderate risk, 16% in the low risk, 8% in the very low risk and 8% in the no risk group.Cytological atypia, necrosis, mucosal invasion and the Ki67 index were found to be related to the progressive disease risk groups of the tumors (p<0.05).Positive immunoreaction was observed with CD117 and PDGFRα in all GISTs in the study (100%). Positivity with the DOG1 antibody was found in 33 (89%) cases. CD34 was positive in 62% (23) of the cases. Conclusion: The CD117 antibody still plays a key role in GIST diagnosis. However, the use of DOG1 and PDGFRα antibodies combined with CD117 as sensitive markers can be beneficial.

References

  • 1. Miettinen M, Lasota J. Gastrointestinal stromal tumors: review on morphology, molecular pathology, prognosis, and differential diagnosis [review]. Arch Pathol Lab Med 2006;130:1466-78.
  • 2. Miettinen M, Wang ZF, Lasota J. DOG1 antibody in the differential diagnosis of gastrointestinal stromal tumors: a study of 1840 cases. Am J Surg Pathol 2009;33:1401-8. [CrossRef]
  • 3. Espinosa I, Lee CH, Kim MK, Rouse BT, Subramanian S, Montgomery K, et al. A novel monoclonal antibody against DOG1 is a sensitive and specific marker for gastrointestinal stromal tumors. Am J Surg Pathol 2008;32:210-8. [CrossRef]
  • 4. West RB, Corless CL, Chen X, Rubin BP, Subramanian S, Montgomery K, et al. The novel marker, DOG1, is expressed ubiquitously in gastrointestinal stromal tumors ırrespective of KIT or PDGFRA mutation status. Am J Pathol 2004;165:107-13. [CrossRef]
  • 5. Ardeleanu C, Arsene D, Hinescu M, Andrei F, Gutu D, Luca L, et al. Pancreatic expression of DOG1 A novel gastrointestinal stromal tumor (GIST) biomarker. Appl Immunohistochem Mol Morphol 2009;17:413-8. [CrossRef]
  • 6. Liegl B, Hornick JL, Corless CL, Fletcher CD. Monoclonal antibody DOG1.1 shows higher sensitivity than KIT in the diagnosis of gastrointestinal stromal tumors, including unusual subtypes. Am J Surg Pathol 2009;33:437-46. [CrossRef]
  • 7. Lopes LF, West RB, Bacchi LM, van de Rijn M, Bacchi CE. DOG1 for the diagnosis of gastrointestinal stromal tumor (GIST): Comparison between 2 different antibodies. Appl Immunohistochem Mol Morphol 2010;18:333-7. [CrossRef]
  • 8. Novelli R, Rossi S, Rodriguez-Justo M, Taniere P, Seddon B, Toffolatti L, et al. DOG1and CD117 are the antibodies of choice in the diagnosis of gastrointestinal stromal tumours. Histopathology 2010;57:259-70. [CrossRef]
  • 9. Miwa S, Nakajima T, Murai Y, Takano Y, Sugiyama T. Mutation assay of the novel gene DOG1 in gastrointestinal stromal tumors (GISTs). J Gastroenterol 2008;43:531-7. [CrossRef]
  • 10. Lee CH, Liang CW, Espinosa I. The utility of discovered on gastrointestinal stromal tumor 1 (DOG1) antibody in surgical pathology-the GIST of it [review]. Adv Anat Pathol 2010;17:222- 32. [CrossRef]
  • 11. Demetri GD, von Mehren M, Antonescu CR, DeMatteo RP, Ganjoo KN, Maki RG, et al. NCCN Task Force Report: Update on the management of patients with Gastrointestinal Stromal Tumors. J Natl Compr Canc Netw 2010;8(Suppl 2):S1-41.
  • 12. Katoh M, Katoh M. FLJ10261 gene, located within the CCND1- EMS1 locus on human chromosome 11q13, encodes the eight-transmembrane protein homologous to C12orf3, C11orf25 and FLJ34272 gene products. Int J Oncol 2003;22:1375-81. [CrossRef]
  • 13. Galindo BE, Vacquier VD. Phylogeny of the TMEM16 protein family: some members are overexpressed in cancer. Int J Mol Med 2005;16:919-24. [CrossRef]
  • 14. Carles A, Millon R, Cromer A, Ganguli G, Lemaire F, Young J, et al. Head and neck squamous cell carcinoma transcriptome analysis by comprehensive validated differential display. Oncogene 2006;25:1821-31. [CrossRef]
  • 15. Wong NA. Gastrointestinal stromal tumours-an update for Histopathologists. Histopathology 2011;59:807-21. [CrossRef]
  • 16. Caputo A, Caci E, Ferrera L, Pedemonte N, Barsanti C, Sondo E, et al. TMEM16A, a membrane protein associated with calciumdependent chloride channel activity. Science 2008;322:590-4. [CrossRef]
There are 16 citations in total.

Details

Other ID JA25KG57SH
Journal Section Research Article
Authors

Beril Güler This is me

Filiz Özyılmaz This is me

Nuray Can This is me

Ebru Taştekin This is me

Burcu Tokuç This is me

Publication Date October 1, 2015
Published in Issue Year 2015 Volume: 32 Issue: 4

Cite

APA Güler, B., Özyılmaz, F., Can, N., Taştekin, E., et al. (2015). Histopathological Features of Gastrointestinal Stromal Tumors and the Contribution of DOG1 Expression to the Diagnosis. Balkan Medical Journal, 32(4), 388-396.
AMA Güler B, Özyılmaz F, Can N, Taştekin E, Tokuç B. Histopathological Features of Gastrointestinal Stromal Tumors and the Contribution of DOG1 Expression to the Diagnosis. Balkan Medical Journal. October 2015;32(4):388-396.
Chicago Güler, Beril, Filiz Özyılmaz, Nuray Can, Ebru Taştekin, and Burcu Tokuç. “Histopathological Features of Gastrointestinal Stromal Tumors and the Contribution of DOG1 Expression to the Diagnosis”. Balkan Medical Journal 32, no. 4 (October 2015): 388-96.
EndNote Güler B, Özyılmaz F, Can N, Taştekin E, Tokuç B (October 1, 2015) Histopathological Features of Gastrointestinal Stromal Tumors and the Contribution of DOG1 Expression to the Diagnosis. Balkan Medical Journal 32 4 388–396.
IEEE B. Güler, F. Özyılmaz, N. Can, E. Taştekin, and B. Tokuç, “Histopathological Features of Gastrointestinal Stromal Tumors and the Contribution of DOG1 Expression to the Diagnosis”, Balkan Medical Journal, vol. 32, no. 4, pp. 388–396, 2015.
ISNAD Güler, Beril et al. “Histopathological Features of Gastrointestinal Stromal Tumors and the Contribution of DOG1 Expression to the Diagnosis”. Balkan Medical Journal 32/4 (October 2015), 388-396.
JAMA Güler B, Özyılmaz F, Can N, Taştekin E, Tokuç B. Histopathological Features of Gastrointestinal Stromal Tumors and the Contribution of DOG1 Expression to the Diagnosis. Balkan Medical Journal. 2015;32:388–396.
MLA Güler, Beril et al. “Histopathological Features of Gastrointestinal Stromal Tumors and the Contribution of DOG1 Expression to the Diagnosis”. Balkan Medical Journal, vol. 32, no. 4, 2015, pp. 388-96.
Vancouver Güler B, Özyılmaz F, Can N, Taştekin E, Tokuç B. Histopathological Features of Gastrointestinal Stromal Tumors and the Contribution of DOG1 Expression to the Diagnosis. Balkan Medical Journal. 2015;32(4):388-96.