Resveratrol Prevented Lipopolysaccharide-Induced Endothelial Dysfunction in Rat Thoracic Aorta Through Increased eNOS Expression

Year 2016, Volume: 33 Issue: 2, 138 - 143, 01.03.2016

Seda Sultan Uğurel Selvinaz Dalaklıoğlu Arda Taşatargil Nilay Kuşçu Çiler Çelik Özenci

Abstract

Background: The cardiovascular benefits of Resveratrol
(RVT) have been well established by previous experimental
and clinical studies.
Aims: The goal of this study was to test the effectiveness
of RVT administration on the impaired endothelial function
induced by lipopolysaccharide (LPS), and to elucidate
the role of endothelial nitric oxide synthase (eNOS)/
Sirtuin 1 (SIRT1) pathway.
Study Design: Animal experiment.
Methods: Endotoxemia was induced by intraperitoneal
injection of 10mg/kg LPS, and the thoracic aorta was
isolated six hours later. RVT was injected intraperitoneally
15 minutes before LPS administration. Six hours
after LPS injection, potassium chloride (KCl), phenylephrine
(Phe), acetylcholine (ACh), and sodium nitroprusside
(SNP) were used to examine to vascular reactivity
and endothelial function. eNOS, phospho-eNOS
(p-eNOS) (Ser 1177), and SIRT1 expressions in thoracic
aorta were evaluated by Western blot.
Results: LPS administration significantly inhibited the
relaxation response induced by ACh, while the relaxation
to SNP was not significantly altered. Phe- and KClinduced
contractile responses in the thoracic aorta significantly
decreased in LPS-injected group. eNOS and
p-eNOS expression decreased significantly in arteries
obtained from LPS group rats. The impaired vasoreactivity
as well as decreased expressions of eNOS, p-eNOS,
and SIRT1 in vessels from LPS-injected rats were improved
by RVT treatment.
Conclusion: The endothelium-dependent vasodilatation
of the thoracic aorta was significantly inhibited by
LPS administration, and RVT treatment may improve
vascular endothelial function. The protective effect of
RVT might be associated with increased eNOS expression
and activity

Keywords

Endothelial dysfunction, nitric oxide synthase type III, lipopolysaccharides, resveratrol, Sirtuin 1

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