Effect of Pregnancy on Vocal Cord Histology: An Animal Experiment

Year 2016, Volume: 33 Issue: 4, 448 - 452, 01.07.2016

Serap Köybaşı Şanal Yusuf Özgür Biçer Aysel Kükner Erkan Tezcan

Abstract

Background: Voice may be affected during the period of pregnancy, especially in the third trimester. However, the exact mechanisms leading to the phonatory changes have not yet uncovered. Study Design: Animal experiment. Methods: Twenty-five Wistar-Albino female rats were divided into four groups: control group, pregnancy day 7 (Group 1), pregnancy day 14 (Group 2) and pregnancy day 20 (Group 3). The laryngeal specimens were obtained under general anesthesia. Histological assessment was performed using Hematoxylin-eosin and toluidine blue. A stereological analysis of vocal cord tissue was performed using a NISElements D32 Imaging Software. Results: Lamina propria was observed to be edematous, and the lamina propria area was thickened starting from the second trimester. Glycosaminoglycans were observed to increase in the second trimester. Although none was encountered in the control, mast cells were observed in the lamina propria layer of the vocal cord starting in the muscular layer in the first trimester proceed to the subepithelial region as degranulated just before term. The covering epithelium remained unchanged throughout pregnancy. Conclusion: Lamina propria thickening may be attributed to both edema and increased glycosaminoglycans. The presence of mast cells in the cordal tissue may induce edema during pregnancy in rats.

Keywords

Pregnancy, voice, mast cell, glycosaminoglycans

References

• 1. Ellegård E, Karlsson G. IgE-mediated reactions and hyperreactivity in pregnancy rhinitis. Arch Otolaryngol Head Neck Surg 1999;125:1121-5. [Crossref]
• 2. Raj A, Gupta B, Chowdhury A, Chadha S. A study of voice changes in various phases of menstrual cycle and in postmenopausal women. J Voice 2010;24:363-8. [Crossref]
• 3. Newman SR, Butler J, Hammond EH, Gray SD. Preliminary report on hormone receptors in the human vocal fold. J Voice 2000;14:72-81. [Crossref]
• 4. Abitbol J. Odessy of the voice. San Diego, CA, USA: Plural Publishing; 2006.
• 5. Brodnitz FS. Menstrual cycle and voice quality. Arch Otolaryngol Head Neck Surg 1979;105:300. [Crossref]
• 6. Higgins MB, Saxman JH. Variations in vocal frequency perturbation across the menstrual cycle. J Voice 1989;3:233-43. [Crossref]
• 7. Cunningham F, Leveno K, Bloom S, Hauth J, Gilstrap III L. Williams Obstetrics. New York, USA: McGraw-Hill; 2005.
• 8. Hamdan AL, Mahfoud L, Sibai A, Seoud M. Effect of pregnancy on the speaking voice. J Voice 2009;23:490-3. [Crossref]
• 9. Cassiraga VL, Castellano AV, Abasolo J, Abin EN, Izbizky GH. Pregnancy and Voice: Changes During the Third Trimester. J Voice 2012;26:584-6. [Crossref]
• 10. Lã FMB, Sundberg J. Pregnancy and the Singing Voice: Reports From a Case Study. J Voice 2012;26:431-9. [Crossref]
• 11. Domeij S, Eriksson A, Dahlqvist A, Forsgren S. Similar distribution of mast cells and substance P-and calcitonin gene-related peptide-immunoreactive nerve fibers in the adult human larynx. Ann Otol Rhinol Laryngol 1996;105:825-31. [Crossref]
• 12. Knöbber D, Agha-Mir-Salim P, Merker H, Jahnke V. [Intraepithelial mast cells in the vocal cords and nasal mucosa]. Laryngorhinootologie 1993;72:590-4. [Crossref]
• 13. Chae SW, Choi G, Kang HJ, Choi JO, Jin SM. Clinical analysis of voice change as a parameter of premenstrual syndrome. J Voice 2001;15:278-83. [Crossref]