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Year 2016, Volume: 33 Issue: 6, 639 - 644, 01.11.2016

Abstract

References

  • 1. Dezateux C, Rosendahl K. Developmental dysplasia of the hip. Lancet 2007:369:1541-52. [CrossRef]
  • 2. Guille JT, Pizzutillo PD, MacEwen GD. Development dysplasia of the hip from birth to six months. J Am Acad Orthop Surg 2000;8:232-42. [CrossRef]
  • 3. Panagiotopoulou N, Bitar K, Hart WJ. The association between mode of delivery and developmental dysplasia of the hip in breech infants: a systematic review of 9 cohort studies. Acta Orthop Belg 2012;78:697-702.
  • 4. Furnes O, Lie SA, Espehaug B, Vollset SE, Engesaeter LB, Havelin LI. Hip disease and the prognosis of total hip replacements. A review of 53,698 primary total hip replacements reported to the Norwegian arthroplasty register 1987-99. J Bone Joint Surg Br 2001;83:579-86. [CrossRef]
  • 5. Chan A, McCaul KA, Cundy PJ, Haan EA, Byron-Scott R. Perinatal risk factors for developmental dysplasia of the hip. Arch Dis Child Neonatal Ed 1997;76:94-100. [CrossRef]
  • 6. Sionek A, Czubak J, Kornacka K, Grabowski B. Evaluation of risk factors in developmental dysplasia of the hip in children from multiple pregnancies: results of hip ultrasonography using Graf’s method. Ortop Traumatol Rehabil 2008;10:115-130.
  • 7. Stein-Zamir C, Volovik, Rishpon S, Sabi R. Developmental dysplasia of the hip: risk markers, clinical screening and outcome. Pediatr Int 2008;50:341-5. [CrossRef]
  • 8. Loening AM, James IE, Levenston ME, Badger AM, Frank EH, Kurz B, et al. Injurious mechanical compression of bovine articular cartilage induces chondrocyte apoptosis. Arch Biochem Biophys 2000;15:205-12. [CrossRef]
  • 9. D’Lima DD, Hashimoto S, Chen PC, Colwell CW Jr, Lotz MK. Human chondrocyte apoptosis in response to mechanical injury. Osteoarthritis Cartilage 2001;9:712-9. [CrossRef]
  • 10. Lotz M, Hashimoto S, Kühn K. Mechanisms of chondrocyte apoptosis. Osteoarthritis Cartilage 1999;7:389-91. [CrossRef]
  • 11. Kouri JB, Aguilera JM, Reyes J, Lozoya KA, González S. Apoptotic chondrocytes from osteoarthrotic human articular cartilage and abnormal calcification of subchondral bone. J Rheumatol 2000;27:1005-19.
  • 12. Hashimoto S, Ochs RL, Komiya S, Lotz M. Linkage of chondrocyte apoptosis and cartilage degradation in human osteoarthritis. Arthritis Rheum 1998;41:1632-8. [CrossRef]
  • 13. Kim HA, Lee YJ, Seong SC, Choe KW, Song YW. Apoptotic chondrocyte death in human osteoarthritis. J Rheumatol 2000;27:455-62.
  • 14. Thomas CM, Fuller CJ, Whittles CE, Sharif Ml. Chondrocyte death by apoptosis is associated with cartilage matrix degradation. Osteoarthritis and Cartilage 2007;15:27-34. [CrossRef]
  • 15. Shapiro F, Connolly S, Zurakowski D, Flynn E, Jaramillo D. Acetabular changes associated with avascular necrosis of the femoral head in a piglet model. Bone Joint Res 2014;3:130-8. [CrossRef]
  • 16. Klisic PJ. Congenital dislocation of the hip-a misleading term: brief report. J Bone Joint Surg Br 1989;71:136.

Altered Chondrocyte Apoptosis Status in Developmental Hip Dysplasia in Rabbits

Year 2016, Volume: 33 Issue: 6, 639 - 644, 01.11.2016

Abstract

Background: Developmental dysplasia of the hip (DDH) is an important factor leading to early adult osteoarthritis. Chondrocyte apoptosis has been proven to be an important factor causing osteoarthritis. Aims: The current study aims to explore whether a rabbit model of developmental dysplasia of the hip through cast immobilization in the legs results in chondrocyte apoptosis.  Study Design: Animal experimentation. Methods: Thirty-two New Zealand white rabbits were divided in three groups with cast plaster-induced dislocation at 2, 4 and 6 weeks. The contralateral hip joint was utilized as a control group. Ten rabbits in each group were sacrificed, and hip specimens were obtained. Bcl-2/Bax, cleaved caspase-3 and cleaved caspase-8 expression were examined by western blot analysis. Chondrocyte apoptosis was analyzed through transmission electron microscopy (TEM) and TUNEL analysis. All experiments were repeated at least three times.  Results: In the experimental group, Bcl-2/Bax, cleaved caspase-3 and cleaved caspase-8 expression were significantly altered. The Bcl-2/Bax ratio decreased with time (all p<0.01), whereas levels of cleaved caspase-3 (p<0.01 and p<0.05) and cleaved caspase-8 (all p<0.05) gradually increased. Chondrocyte apoptosis was observed through transmission electron microscopy (TEM) and TUNEL analysis (p<0.05 at 4 weeks and p<0.01 at 6 weeks).  Conclusion: Prolonged immobilization of rabbit hip caused chondrocyte apoptosis. Reduction of the hip joint may protect chondrocytes from apoptosis, thus preventing secondary osteoarthritis.

References

  • 1. Dezateux C, Rosendahl K. Developmental dysplasia of the hip. Lancet 2007:369:1541-52. [CrossRef]
  • 2. Guille JT, Pizzutillo PD, MacEwen GD. Development dysplasia of the hip from birth to six months. J Am Acad Orthop Surg 2000;8:232-42. [CrossRef]
  • 3. Panagiotopoulou N, Bitar K, Hart WJ. The association between mode of delivery and developmental dysplasia of the hip in breech infants: a systematic review of 9 cohort studies. Acta Orthop Belg 2012;78:697-702.
  • 4. Furnes O, Lie SA, Espehaug B, Vollset SE, Engesaeter LB, Havelin LI. Hip disease and the prognosis of total hip replacements. A review of 53,698 primary total hip replacements reported to the Norwegian arthroplasty register 1987-99. J Bone Joint Surg Br 2001;83:579-86. [CrossRef]
  • 5. Chan A, McCaul KA, Cundy PJ, Haan EA, Byron-Scott R. Perinatal risk factors for developmental dysplasia of the hip. Arch Dis Child Neonatal Ed 1997;76:94-100. [CrossRef]
  • 6. Sionek A, Czubak J, Kornacka K, Grabowski B. Evaluation of risk factors in developmental dysplasia of the hip in children from multiple pregnancies: results of hip ultrasonography using Graf’s method. Ortop Traumatol Rehabil 2008;10:115-130.
  • 7. Stein-Zamir C, Volovik, Rishpon S, Sabi R. Developmental dysplasia of the hip: risk markers, clinical screening and outcome. Pediatr Int 2008;50:341-5. [CrossRef]
  • 8. Loening AM, James IE, Levenston ME, Badger AM, Frank EH, Kurz B, et al. Injurious mechanical compression of bovine articular cartilage induces chondrocyte apoptosis. Arch Biochem Biophys 2000;15:205-12. [CrossRef]
  • 9. D’Lima DD, Hashimoto S, Chen PC, Colwell CW Jr, Lotz MK. Human chondrocyte apoptosis in response to mechanical injury. Osteoarthritis Cartilage 2001;9:712-9. [CrossRef]
  • 10. Lotz M, Hashimoto S, Kühn K. Mechanisms of chondrocyte apoptosis. Osteoarthritis Cartilage 1999;7:389-91. [CrossRef]
  • 11. Kouri JB, Aguilera JM, Reyes J, Lozoya KA, González S. Apoptotic chondrocytes from osteoarthrotic human articular cartilage and abnormal calcification of subchondral bone. J Rheumatol 2000;27:1005-19.
  • 12. Hashimoto S, Ochs RL, Komiya S, Lotz M. Linkage of chondrocyte apoptosis and cartilage degradation in human osteoarthritis. Arthritis Rheum 1998;41:1632-8. [CrossRef]
  • 13. Kim HA, Lee YJ, Seong SC, Choe KW, Song YW. Apoptotic chondrocyte death in human osteoarthritis. J Rheumatol 2000;27:455-62.
  • 14. Thomas CM, Fuller CJ, Whittles CE, Sharif Ml. Chondrocyte death by apoptosis is associated with cartilage matrix degradation. Osteoarthritis and Cartilage 2007;15:27-34. [CrossRef]
  • 15. Shapiro F, Connolly S, Zurakowski D, Flynn E, Jaramillo D. Acetabular changes associated with avascular necrosis of the femoral head in a piglet model. Bone Joint Res 2014;3:130-8. [CrossRef]
  • 16. Klisic PJ. Congenital dislocation of the hip-a misleading term: brief report. J Bone Joint Surg Br 1989;71:136.
There are 16 citations in total.

Details

Other ID JA97ND85RE
Journal Section Research Article
Authors

Yi-shan Wei This is me

Dai-he Li This is me

Wan-lin Liu This is me

Dian-ming Jiang This is me

Publication Date November 1, 2016
Published in Issue Year 2016 Volume: 33 Issue: 6

Cite

APA Wei, Y.-s., Li, D.-h., Liu, W.-l., Jiang, D.-m. (2016). Altered Chondrocyte Apoptosis Status in Developmental Hip Dysplasia in Rabbits. Balkan Medical Journal, 33(6), 639-644.
AMA Wei Ys, Li Dh, Liu Wl, Jiang Dm. Altered Chondrocyte Apoptosis Status in Developmental Hip Dysplasia in Rabbits. Balkan Medical Journal. November 2016;33(6):639-644.
Chicago Wei, Yi-shan, Dai-he Li, Wan-lin Liu, and Dian-ming Jiang. “Altered Chondrocyte Apoptosis Status in Developmental Hip Dysplasia in Rabbits”. Balkan Medical Journal 33, no. 6 (November 2016): 639-44.
EndNote Wei Y-s, Li D-h, Liu W-l, Jiang D-m (November 1, 2016) Altered Chondrocyte Apoptosis Status in Developmental Hip Dysplasia in Rabbits. Balkan Medical Journal 33 6 639–644.
IEEE Y.-s. Wei, D.-h. Li, W.-l. Liu, and D.-m. Jiang, “Altered Chondrocyte Apoptosis Status in Developmental Hip Dysplasia in Rabbits”, Balkan Medical Journal, vol. 33, no. 6, pp. 639–644, 2016.
ISNAD Wei, Yi-shan et al. “Altered Chondrocyte Apoptosis Status in Developmental Hip Dysplasia in Rabbits”. Balkan Medical Journal 33/6 (November 2016), 639-644.
JAMA Wei Y-s, Li D-h, Liu W-l, Jiang D-m. Altered Chondrocyte Apoptosis Status in Developmental Hip Dysplasia in Rabbits. Balkan Medical Journal. 2016;33:639–644.
MLA Wei, Yi-shan et al. “Altered Chondrocyte Apoptosis Status in Developmental Hip Dysplasia in Rabbits”. Balkan Medical Journal, vol. 33, no. 6, 2016, pp. 639-44.
Vancouver Wei Y-s, Li D-h, Liu W-l, Jiang D-m. Altered Chondrocyte Apoptosis Status in Developmental Hip Dysplasia in Rabbits. Balkan Medical Journal. 2016;33(6):639-44.