Abstract
Boron has significant impact on the mineral composition of teeth. Copine-7 (Cpne-7) is secreted by pre-ameloblasts and induces dentin formation via differentiation of mesenchymal cells of dental. The goal of this study was to see how boric acid affected the bioactivity and expression of Cpne-7, collagen type I in dental pulp-obtained stem cell. The expression of Cpne-7, COL-I were assessed in the boric acid treated pulp cells by molecular method on 3 and 8 days exposure. When comparing the different boric acid concentrations to the control group in a proliferation experiment, no significant differences were noted. At 10 ng/mL boric acid, a rise in the number of mineralized nodules was observed. Boric acid concentrations (1 ng/mL, 10 ng/mL) increased the transcripts of Cpne-7 on day 3 and 8. Additionally, 1 ng/mL boric acid concentration significantly upregulated of Cpne-7 expression compared to control group on day 3 and 8. When the 1-10 ng boric acid was compared to control group COL-I expression remarkably enhanced in cell. According to the current findings that boric acid may be a potent regulator of Cpne-7, which is a promising candidate for new dentin formation in regenerative dentistry, in dental pulp-obtained mesenchymal stem cells and provide osteogenic efficacy in therapies aimed at dentin formation.
Keywords
References
- 1. Park, Y-H., Lee, Y.S., Park, J-S., Kim, S.H., Bae, H.S., & Park, J-C. (2019). Expression of CPNE7 during mouse dentinogenesis. Journal of Molecular Histology, 50, 179-188.
Abstract
Boron has significant impact on the mineral composition of teeth. Copine-7 (Cpne-7) is secreted by pre-ameloblasts and induces dentin formation via differentiation of mesenchymal cells of dental. The goal of this study was to see how boric acid affected the bioactivity and expression of Cpne-7, collagen type I in dental pulp-obtained stem cell. The expression of Cpne-7, COL-I were assessed in the boric acid treated pulp cells by molecular method on 3 and 8 days exposure. When comparing the different boric acid concentrations to the control group in a proliferation experiment, no significant differences were noted. At 10 ng/mL boric acid, a rise in the number of mineralized nodules was observed. Boric acid concentrations (1 ng/mL, 10 ng/mL) increased the transcripts of Cpne-7 on day 3 and 8. Additionally, 1 ng/mL boric acid concentration significantly upregulated of Cpne-7 expression compared to control group on day 3 and 8. When the 1-10 ng boric acid was compared to control group COL-I expression remarkably enhanced in cell. According to the current findings that boric acid may be a potent regulator of Cpne-7, which is a promising candidate for new dentin formation in regenerative dentistry, in dental pulp-obtained mesenchymal stem cells and provide osteogenic efficacy in therapies aimed at dentin formation.
Keywords
References
- 1. Park, Y-H., Lee, Y.S., Park, J-S., Kim, S.H., Bae, H.S., & Park, J-C. (2019). Expression of CPNE7 during mouse dentinogenesis. Journal of Molecular Histology, 50, 179-188.
Details
| Primary Language | English |
|---|---|
| Journal Section | Research Article |
| Authors | |
| Publication Date | June 30, 2022 |
| Acceptance Date | April 18, 2022 |
| Published in Issue | Year 2022 |
Journal of Boron by Turkish Energy Nuclear Mineral Research Agency is licensed under CC BY-NC-SA 4.0
