The Effect of boric acid on nicotine-related deprive of bioactivity in cementoblast cells

Abstract

Boric acid (BA) affects alveolar bone density related to mineral composition in dental physiology and nicotine (N) exposure is an important risk factor in the pathophysiology of periodontal disease. The aim of this study was to investigate the effect of BA on the deprive of bioactivity in cementoblast cells after N exposure. For this purpose, cementoblast cells were treated with different concentrations (10-1, 10-2, 10-3, 10-4, 10-5, 10-6 mM) of N and BA at a concentration of 10 ng/mL and cell viability was evaluated at 24 and 72 h by MTT assay. The migration potential of cells into the wound area was visualized by migration:wound healing assay. Total RNA was isolated from cells on days 3, 6 and 9 and gene expression of mineralized tissue-associated markers was analyzed using real-time polymerase chain reaction. The results showed that N had a significant effect on the viability of cementoblast cells, migration-based wound healing, especially on the expression of mineralized tissue-related genes. In addition, it was determined that target gene expression patterns were negatively affected with the increase in N exposure on a daily basis, and BA application may be a powerful preventive therapeutic agent in the protection of cementoblast cell functions.

Keywords

• Boric acid
• Nicotin
• Cementoblast

Project Number

1919B012104849

Borik asitin sementoblast hücrelerinde nikotin kaynaklı biyoaktivite kaybı üzerindeki etkisi

Abstract

Borik asit (BA) dental fizyolojide mineral kompozisyonu ile ilişkili alveolar kemik yoğunluğunu etkiler ve nikotin (N) maruziyeti periodontal hastalığın patofizyolojisinde önemli bir risk faktörüdür. Bu çalışmanın amacı, BA'in sementoblast hücrelerinde N maruziyeti sonrası biyoaktivite kaybı üzerindeki etkisini araştırmaktır. Bu amaçla, sementoblast hücreleri farklı konsantrasyonlarda (10-1, 10-2, 10-3, 10-4, 10-5, 10-6 mM) N ve 10 ng/mL konsantrasyonda BA ile muamele edilmiş ve hücre canlılığı 24 ve 72. saatlerde MTT testi ile değerlendirildi. Hücrelerin yara alanına göç potansiyeli, göç:yara iyileşmesi deneyi ile görüntülendi. Total RNA 3, 6 ve 9. günlerde hücrelerden izole edildi ve mineralize doku ile ilişkili belirteçlerin gen ekspresyonu gerçek zamanlı polimeraz zincir reaksiyonu kullanılarak analiz edildi. Elde edilen sonuçlar, N'in sementoblast hücrelerinin canlılığı, migrasyona dayalı yara iyileşmesi ve özellikle mineralize doku ile ilgili genlerin ekspresyonu üzerinde önemli bir etkiye sahip olduğunu gösterdi. Ayrıca N maruziyetinin gün bazında artmasıyla birlikte hedef gen ifade paternlerinin olumsuz etkilendiği ve BA uygulamasının ise sementoblast hücre fonksiyonlarının korunmasında güçlü bir prevantif terapötik ajan olabileceği belirlendi.

Keywords

• Borik asit
• Nikotin
• Sementoblast

Supporting Institution

TÜBİTAK

Project Number

1919B012104849

Thanks

Bu makale TÜBİTAK 2209-A Üniversite Öğrencileri Araştırma Projeleri Destek Programı kapsamında 1919B012104849 numaralı proje ile desteklenmiştir. Vermiş oldukları destekle bu projeyi gerçekleştirmemizi sağlayan TÜBİTAK'a teşekkür ederiz.

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