2-formilfenilboronik ve 3-klorofenilboronik asit türevlerinin in vitro sitotoksisite ve hücre migrasyonu açısından değerlendirilmesi

Abstract

Yara tedavisi ve cildin yenilenmesi karmaşık bir proses olup, iyileşmeyen yaralar sağlık açısından büyük bir sosyoekonomik yük teşkil etmektedir. Başarılı bir yara yönetimi için etkili ve alternatif yaklaşımlara ihtiyaç bulunmaktadır. Boranik asit türevlerinin yara iyileşmesine yönelik olumlu ve güçlü etkileri rapor edilmekle beraber, fenil ikameli boranik asit türevleri iyileşmede daha rejeneratif ve etkili bileşikler olarak kullanılabilir. Bu çalışmada 2-formyl phenyl boronic acid ve 3-chloro phenyl boronic acid’in L929 fibroblast hücre hatları üzerine in vitro sitotoksik etkileri WST-8 analiziyle ve yara iyileştirici etkileri hücre göçü testi ile araştırılmıştır. Verilerimiz her iki boranik asit türevinin de konsantrasyon bağımlı etkilerini ortaya koymaktadır. 2-formyl phenyl boronic acid için 3.90-31.25 µg/ml arası doz uygulamaları % 84 ve üzerinde bir canlılık göstermiş, daha yüksek konsantrasyonlarda ise canlılık % 5-10 oranında bulunmuştur. 3-chloro phenyl boronic acid için 3.90-250 µg/ml arası doz uygulamaları sonucunda % 64-109 oranında bir canlılık gözlenirken, 500 µg/ml konsantrasyonda canlılık yüzdesi % 17’ye düşmektedir. Cell migration testi verileri, PBA türevlerinin hücre göçü açısından etkilerinin zaman arttıkça arttığını, 2-formyl phenyl boronic asitin 24. saatteki etkisinin hücre göçü açısından oldukça etkili olduğunu göstermektedir. PBA türevlerinin yara iyileştirici etkisi konsantrasyon bağımlı olduğundan, yüksek konsantrasyonların kullanımının toksik olabileceği de gözönünde bulundurulmalıdır.

Keywords

• 2-formil fenil boronik asit
• 3-kloro fenil boronik asit
• in vitro sitotoksisite
• yara iyileşmesi

Evaluation of 2-formylphenylboronic and 3-chlorophenylboronic acid derivatives for in vitro cytotoxicity and cell migration

Abstract

Wound treatment and skin regeneration are complex processes, and non-healing wounds pose a major socioeconomic burden in terms of health. Effective and alternative approaches are needed for successful wound management. Although boronic acid derivatives have been reported to have positive and strong effects on wound healing, phenyl-substituted boronic acid derivatives can be used as more regenerative and effective compounds in healing. In this study, the in vitro cytotoxic effects of 2-formylphenylboronic acid and 3-chlorophenylboronic acid on L929 fibroblast cell lines were investigated using WST-8 analysis, and their wound healing effects were investigated by cell migration test. Our data reveal concentration-dependent effects of both boronic acid derivatives. For 2-formylphenylboronic acid, dosage applications between 3.90-31.25 μg/ml showed a viability of 84% and above, and at higher concentrations, the viability was found to be 5-10%. For 3-chlorophenylboronic acid, a viability rate of 64-109% is observed as a result of dosage applications between 3.90-250 μg/ml, while the percentage of viability decreases to 17% at a concentration of 500 μg/ml. Cell migration test data show that the effects of phenylboronic acid (PBA) derivatives in terms of cell migration increase as time increases, and the effect of 2-formylphenylboronic acid at the 24th hour is quite effective in terms of cell migration. Since the wound healing effect of PBA derivatives is concentration dependent, it should be taken into consideration that the use of high concentrations may be toxic.

Keywords

• 2-formyl phenyl boronic acid
• 3-chloro phenyl boronic acid
• in vitro cytotoxicity
• wound healing

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