Bor stresi altında Arabidopsis thaliana (L.) Heynh’da süperoksit dismutaz genlerinin ekspresyon profillerinin belirlenmesi

Abstract

Borun topraktaki eksikliği veya yüksek konsantrasyonda bulunması önemli bir abiyotik stres faktörü olabilmektedir. Arabidopsis thaliana^’da Cu/ZnSOD (CSD1, CDS2, CSD3), FeSOD (FSD1, FSD2, FSD3) ve MnSOD (MSD1) genlerinden oluşan 7 farklı süperoksit dismutaz (SOD) izoformu tanımlanmıştır. Yapılan çalışmada amaç bor (B) eksikliği veya toksisite koşulları altında Arabidopsis thaliana (L.)’nın yaprak ve kök dokusunda antioksidan sistemin anahtar enzimi olan süperoksit dismutazı kodlayan genlerin ifade düzeylerini Real-Time PCR ile belirlenmesidir. Arabidopsis bitkileri altı hafta hidroponik kültür sisteminde yetiştirilmiş ve 48 saat süreyle borik asit (BA)  içermeyen (0 µM) veya yüksek konsantrasyonda BA (3000 µM) içeren besin ortamlarında inkübe edilmiştir. B stres uygulamaları CSD1 ve FSD3 genlerinin ekspresyonunu yaprak dokusunda arttırmıştır. MSD1 geninin mRNA seviyesi yaprak dokusunda B toksisite uygulaması sonucu artarken, kök dokusunda B eksikliğinde artış göstermiştir. B eksikliği ve toksisitesi uygulamaları CSD2, FSD1 ve FSD2 genlerinin ekspresyon düzeyini dokuya bağlı olarak değiştirmiştir. Sonuç olarak, B stresinin kök ve yapraklarda farklı hücresel kompartımanlarda antioksidatif savunmayı tetiklediği ortaya koyulmuştur.

Keywords

• Arabidopsis thaliana L.,bor eksikliği,bor toksisitesi,gen ekspresyonu,süperoksit dismutaz

Determination of expression profiles of superoxide dismutase genes in Arabidopsis thaliana (L.) Heynh under boron stress

Abstract

Deficiency or excess of boron in the soil may be an important abiotic stress factor. Seven different superoxide dismutase (SOD) isoforms including Cu/ZnSOD (CSD1, CDS2, CSD3), FeSOD (FSD1, FSD2, FSD3), and MnSOD (MSD1) genes have been identified in Arabidopsis thaliana. The aim of this study is to determine the expression levels of the genes encoding superoxide dismutase, which is the key enzyme of the antioxidant system in leaf and root tissues of Arabidopsis thaliana (L.) under boron (B) deficiency or toxicity conditions using Real-Time PCR. Arabidopsis plants were grown in hydroponic culture system for six weeks and incubated for 48 hours in mediums excluding boric acid (BA) (0 µM) or including high concentration of BA (3000 µM). B stress tretaments increased the expression levels of the CSD1 and FSD3 genes in leaf tissue. mRNA level of MSD1 gene is increased in result of B toxicity application in leaf tissue, while in root tissue B deficiency application increased the mRNA level. B deficiency and toxicity treatments altered the expression levels of the CSD2, FSD1, and FSD2 genes depending on the tissue. In conclusion, it has been revealed that B stress triggers antioxidative defense mechanisms in different cellular compartments in roots and leaves.

Keywords

• Arabidopsis thaliana L.,boron deficiency,boron toxicity,gene expression,superoxide dismutase

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