Investigation of the therapy potential of borax pentahydrate in glioblastoma multiforme cell line

Abstract

Recent studies with natural and synthetic boron compounds suggest that these compounds may be effective in the prevention and treatment of cancer. In this context, a synthetic boron compound, Borax pentahydrate (BPH), is aimed to be tested for cytotoxic effect on the glioblastoma multiforme (GBM) model U-87MG cell line representing fourth stage brain cancer in terms of induction of apoptosis and autophagy. Changes in apoptosis and autophagy rates of the research group treated with the specified IC50 dose of BPH were determined by fluorescence-based microcapillary cytometry kit (Muse® Annexin V & Cell Death and Muse® Autophagy LC3-antibody based kit). The data obtained were evaluated using GraphPad Prism 5 statistical program. The IC50 value of BPH in the U-87MG cell line was determined to be 2454μM for cytotoxicity. On the other hand, apoptosis rate was determined as increased 12.79 folds in the BPH treated group compared to the control group, while elevation of autophagy rate is determined as 1.2 folds. In the light of these data, while the cytotoxic effect of BPH on U-87MG cells, which is a model for GBM, is designated, it is understood that cell death pattern occurs through apoptosis instead of autophagy. In addition to these, it is thought that with the support of other new studies BPH may be an alternative agent that can be used in the treatment of GBM.

Keywords

• Apoptosis,Borax pentahydrate,Glioblastoma multiforme,Autophagy,U-87 MG

Project Number

113S700

Boraks pentahidrat'ın glioblastoma multiforme hücre hattındaki tedavi potansiyelinin araştırılması

Abstract

Doğal ve sentetik bor bileşikleri ile yapılan son dönemdeki araştırmalar bu bileşiklerin kanserden korunma ve tedavisinde etkin olabileceğini işaret etmektedir. Bu bağlamda, sentetik bir bor bileşiği olan Boraks pentahidrat (BPH)’ın dördüncü evre beyin kanserini temsil eden glioblastoma multiforme (GBM) modeli U-87MG hücre hattı üzerindeki sitotoksik etkisi ile apoptoz ve otofaji indüksiyonu yönünden sınanması amaçlanmıştır. BPH’ın belirlenmiş IC50 dozu ile muamele edilmiş çalışma grubunun apoptoz ve otofaji oranlarındaki değişimler floresan temelli mikrokapiller sitometri cihazına uygun kitler (Muse® Annexin V & Hücre Ölümü ve Muse® Otofaji LC3-antikor temelli) aracılığı ile belirlenmiştir. Elde edilen veriler, GraphPad Prism 5 istatistik programı kullanılarak değerlendirilmiştir. Sitotosisite yönünden BPH’ın U-87 MG hücre hattındaki IC50 değeri 2454 μM olarak belirlenmiştir. Diğer taraftan kontrol grubuna oranla BPH ile muamele edilmiş grupta apoptoz oranı 12,79 kat iken otofaji oranının ise 1,2 kat arttığı saptanmıştır. Bu veriler ışığında, BPH’ın GBM’ye model oluşturan U-87 MG hücrelerine sitotoksik etkisi bilenirken, hücre ölümü biçiminin otofaji yerine apoptoz üzerinden gerçekleştiği anlaşılmaktadır. Bütün bunlarla birlikte, BPH’ın yeni araştırmalarla desteklenerek GBM tedavisinde kullanılabilecek alternatif bir ajan olabileceği düşünülmektedir.

Keywords

• Apoptoz,Boraks Pentahidrat,Glioblastoma multiforme,Otofaji,U-87 MG

Supporting Institution

TÜBİTAK

Project Number

113S700

Thanks

Teşekkür ederiz.

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