Bor stresi altında Arabidopsis thaliana (L.) Heynh’da süperoksit dismutaz genlerinin ekspresyon profillerinin belirlenmesi

Year 2018, Volume: 3 Issue: 3, 145 - 150, 30.11.2018

Yonca Surgun Acar , Rabia İşkil Yavuz Erden

https://doi.org/10.30728/boron.409349

Abstract

Borun topraktaki eksikliği veya yüksek konsantrasyonda
bulunması önemli bir abiyotik stres faktörü olabilmektedir. Arabidopsis thaliana^’da Cu/ZnSOD (CSD1, CDS2, CSD3), FeSOD (FSD1, FSD2, FSD3) ve MnSOD (MSD1) genlerinden oluşan 7 farklı
süperoksit dismutaz (SOD) izoformu tanımlanmıştır. Yapılan çalışmada amaç bor (B)
eksikliği veya toksisite koşulları altında Arabidopsis
thaliana (L.)’nın yaprak ve kök dokusunda antioksidan sistemin anahtar
enzimi olan süperoksit dismutazı kodlayan genlerin ifade düzeylerini Real-Time
PCR ile belirlenmesidir. Arabidopsis bitkileri altı hafta hidroponik
kültür sisteminde yetiştirilmiş ve 48 saat süreyle borik asit (BA)  içermeyen (0 µM) veya yüksek konsantrasyonda BA
(3000 µM) içeren besin ortamlarında inkübe edilmiştir. B stres uygulamaları CSD1 ve FSD3 genlerinin ekspresyonunu yaprak dokusunda arttırmıştır. MSD1 geninin mRNA seviyesi yaprak
dokusunda B toksisite uygulaması sonucu artarken, kök dokusunda B eksikliğinde artış
göstermiştir. B eksikliği ve toksisitesi uygulamaları CSD2, FSD1 ve FSD2 genlerinin ekspresyon düzeyini dokuya
bağlı olarak değiştirmiştir. Sonuç olarak, B stresinin kök ve yapraklarda farklı
hücresel kompartımanlarda antioksidatif savunmayı tetiklediği ortaya
koyulmuştur.

Keywords

Arabidopsis thaliana L., bor eksikliği, bor toksisitesi, gen ekspresyonu, süperoksit dismutaz

Determination of expression profiles of superoxide dismutase genes in Arabidopsis thaliana (L.) Heynh under boron stress

Abstract

Deficiency or excess
of boron in the soil may be an important abiotic stress factor. Seven different
superoxide dismutase (SOD) isoforms including Cu/ZnSOD (CSD1, CDS2, CSD3), FeSOD (FSD1, FSD2, FSD3), and MnSOD (MSD1) genes have been identified
in Arabidopsis thaliana. The aim of
this study is to determine the expression levels of the genes encoding
superoxide dismutase, which is the key enzyme of the antioxidant system in leaf
and root tissues of Arabidopsis thaliana
(L.) under boron (B) deficiency or toxicity conditions using Real-Time
PCR. Arabidopsis plants were grown in hydroponic culture system for six
weeks and incubated for 48 hours in mediums excluding boric acid (BA) (0 µM) or
including high concentration of BA (3000 µM). B stress tretaments
increased the expression levels of the CSD1
and FSD3 genes in leaf tissue. mRNA level of MSD1 gene is increased in result of B
toxicity application in leaf tissue, while in root tissue B deficiency application
increased the mRNA level. B deficiency and toxicity treatments altered
the expression levels of the CSD2, FSD1,
and FSD2 genes depending on the
tissue. In conclusion, it has been revealed that B stress triggers
antioxidative defense mechanisms in different cellular compartments in roots
and leaves.

Keywords

Arabidopsis thaliana L., boron deficiency, boron toxicity, gene expression, superoxide dismutase

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