Research Article
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Year 2023, , 275 - 280, 01.05.2023
https://doi.org/10.47115/bsagriculture.1269682

Abstract

References

  • Aghelan Z, Shariat SZS. 2015. Partial purification and biochemical characterization of peroxidase from rosemary (Rosmarinus officinalis L.) leaves. Adv Biomed Res, 4.
  • Al-Senaidy AM, Ismael MA. 2011. Purification and characterization of membrane-bound peroxidase from date palm leaves (Phoenix dactylifera L.). Saudi J Biol Sci, 18: 293-298.
  • Ashie INA, Simpson BK, Smith JP. 1996. Mechanisms for controlling enzymatic reactions in foods. Crit Rev Food Sci Nutr, 36: 1-30.
  • Barrett DM, Beaulieu JC, Shewfelt R 2010. Color, flavor, texture, and nutritional quality of fresh-cut fruits and vegetables: desirable levels, instrumental and sensory measurement, and the effects of processing. Crit Rev Food Sci Nutr, 50: 369-389.
  • Bursal E. 2013. Kinetic properties of peroxidase enzyme from chard (Beta vulgaris Subspecies cicla) leaves. Int J Food Prop, 16: 1293-1303.
  • Castrejón ADR, Eichholz I, Rohn S, Kroh LW, Huyskens-Keil S. 2008. Phenolic profile and antioxidant activity of highbush blueberry (Vaccinium corymbosum L.) during fruit maturation and ripening. Food Chem, 109: 564-572.
  • Dasgupta P, Chakraborty P, Bala NN. 2013. Averrhoa carambola: an updated review. Int J Pharm Res, 2: 54-63.
  • Erdem HÜ, Kalın R, Özdemir N, Özdemir H. 2015. Purification and biochemical characterization of peroxidase isolated from white cabbage (Brassica oleracea var. capitata f. alba). Int J Food Prop, 18: 2099-2109.
  • Franzini L, Ardigo D, Valtuena S, Pellegrini N, Del Rio D, Bianchi MA, Scazzina F, Piatti PM, Brighenti F, Zavaroni I. 2012. Food selection based on high total antioxidant capacity improves endothelial function in a low cardiovascular risk population. Nutr Metab Cardiovasc Dis, 22: 50-57.
  • Gelen V, Şengül E, Yıldırım S, Senturk E, Tekin S, Kükürt A. 2021. The protective effects of hesperidin and curcumin on 5-fluorouracil–induced nephrotoxicity in mice. Environ Sci Pollut Res, 28: 47046-47055.
  • Gupta S K, Sharma M, Deeba F, Pandey V. 2017. Role of reactive oxygen species in photophosphorylation and damage to D1 protein: past and present. Reactive Oxygen Species in Plants: Boon Or Bane‐Revisiting the Role of ROS, 165-186.
  • Herath N, Kodithuwakku G, Dissanayake T, Rathnathunga N, Weerakoon K. 2021. Acute Kidney Injury Following Star Fruit Ingestion: A Case Series. Wilderness Environ Med, 32: 98-101.
  • Hu Y, Wu J, Luo P, Mo Y. 2012. Purification and partial characterization of peroxidase from lettuce stems. Afr J Biotechnol, 11: 2752-2756.
  • Huang R, Xia R, Hu L, Lu Y, Wang M. 2007. Antioxidant activity and oxygen-scavenging system in orange pulp during fruit ripening and maturation. Sci Hortic, 113: 166-172.
  • Ikram EHK, Eng KH, Jalil AMM, Ismail A, Idris S, Azlan A, Nazri HSM, Diton NAM, Mokhtar, RAM. 2009. Antioxidant capacity and total phenolic content of Malaysian underutilized fruits. J Food Compos Anal, 22: 388-393.
  • Ilahy R, Hdider C, Lenucci MS, Tlili I, Dalessandro G. 2011. Antioxidant activity and bioactive compound changes during fruit ripening of high-lycopene tomato cultivars. J Food Compos Anal, 24: 588-595.
  • Isabelle M, Lee BL, Lim MT, Koh WP, Huang D, Ong CN. 2010. Antioxidant activity and profiles of common fruits in Singapore. Food Chem, 123: 77-84.
  • Koksal E, Bursal E, Aggul AG, Gulcin I. 2012. Purification and characterization of peroxidase from sweet gourd (Cucurbita Moschata Lam. Poiret). Int J Food Prop, 15: 1110-1119.
  • Lampila P, van Lieshout M, Gremmen B, Lähteenmäki L 2009. Consumer attitudes towards enhanced flavonoid content in fruit. Int Food Res J, 42: 122-129.
  • Lavery CB, MacInnis MC, MacDonald MJ, Williams JB, Spencer CA, Burke AA, Irwin DJG, D’Cunha GB. 2010. Purification of peroxidase from horseradish (Armoracia rusticana) roots. J Agric Food Chem, 58: 8471-8476.
  • Leong LP, Shui G. 2002. An investigation of antioxidant capacity of fruits in Singapore markets. Food Chem, 76: 69-75.
  • López-Serrano M, Barceló AR. 1996. Purification and characterization of a basic peroxidase isoenzyme from strawberries. Food Chem, 55: 133-137.
  • Loukili AE, Limam F, Ayadi A, Boyer N, Ouelhazi L. 1999. Purification and characterization of a neutral peroxidase induced by rubbing tomato internodes. Physiol Plant, 105: 24-31.
  • Maciel HPF, Gouvêa CMCP, Toyama M, Smolka M, Marangoni S, Pastore GM. 2007. Extraction, purification and biochemical characterization of a peroxidase from Copaifera langsdorffii leaves. Quim Nova, 30: 1067-1071.
  • Mafulul SG, Joel EB, Barde LA, Lenka JL, Ameh AA, Phililus MG. 2018. Extraction, partial purification and characterization of peroxidase from Calotropis procera leaves. J adv biol biotechnol, 18: 1-10.
  • Manda H, Vyas K, Pandya A, Singhal G. 2012. A complete review on: Averrhoa carambola. World J Pharm Pharm Sci, 1: 17-33.
  • Manu BT, Rao UP. 2009. Calcium modulated activity enhancement and thermal stability study of a cationic peroxidase purified from wheat bran. Food Chem, 114: 66-71.
  • Marnewick JL, Rautenbach F, Venter I, Neethling H, Blackhurst DM, Wolmarans P, Macharia M. 2011. Effects of rooibos (Aspalathus linearis) on oxidative stress and biochemical parameters in adults at risk for cardiovascular disease. J Ethnopharmacol, 133: 46-52.
  • Muthu N, Lee SY, Phua KK, Bhore SJ. 2016. Nutritional, medicinal and toxicological attributes of star-fruits (Averrhoa carambola L.): a review. Bioinformation, 12: 420.
  • O'Dougherty M, Harnack LJ, French SA, Story M, Oakes JM, Jeffery RW. 2006. Nutrition labeling and value size pricing at fast-food restaurants: a consumer perspective. Am J Health Promot, 20: 247-250.
  • Olson KR, Straub KD. 2015. The role of hydrogen sulfide in evolution and the evolution of hydrogen sulfide in metabolism and signaling. Physiology, 31: 60-72
  • Park YK, Park E, Kim JS, Kang MH. 2003. Daily grape juice consumption reduces oxidative DNA damage and plasma free radical levels in healthy Koreans. Mutat Res Fundam Mol Mech Mutagen, 529: 77-86.
  • Passardi F, Theiler G, Zamocky M, Cosio C, Rouhier N, Teixera F, Margis-Pinheiro M, Ioannidis V, Penel C, Falquet L, Dunand C. 2007. PeroxiBase: the peroxidase database. Phytochem, 68: 1605-1611.
  • Rautiainen S, Levitan EB, Mittleman MA, Wolk A. 2013. Total antioxidant capacity of diet and risk of heart failure: a population-based prospective cohort of women. Am J Med, 126: 494-500.
  • Sakharov IY, Castillo JL, Areza JC, Galaev IY. 2000. Purification and stability of peroxidase of African oil palm Elaies guineensis. Bioseparation, 9: 125-132.
  • Sengul E, Gelen V, Yildirim S, Senturk E, Dag Y, Eser G, Gok M. 2021. Investigation of effects of Silymarin in 5-fluorouracil hepatotoxicity and nephrotoxicity in Mice.
  • Sewelam N, Kazan K, Schenk PM. 2016. Global plant stress signaling: reactive oxygen species at the cross-road. Front Plant Sci, 7: 187.
  • Shofian NM, Hamid AA, Osman A, Saari N, Anwar F, Pak Dek MS, Hairuddin MR. 2011. Effect of freeze-drying on the antioxidant compounds and antioxidant activity of selected tropical fruits. Int J Mol Sci, 12: 4678-4692.
  • Shui G, Wong SP, Leong LP. 2004. Characterization of antioxidants and change of antioxidant levels during storage of Manilkara zapota L. J Agric Food Chem, 52: 7834-7841.
  • Smith IK, Vierheller TL, Thorne CA. 1988. Assay of glutathione reductase in crude tissue homogenates using 5, 5′-dithiobis (2-nitrobenzoic acid). Anal. Biochem, 175: 408-413.
  • Şişecioğlu M, Gülçin İ, Cankaya M, Atasever A, Şehitoğlu MH, Kaya HB, Özdemir H. 2010. Purification and characterization of peroxidase from Turkish black radish (Raphanus sativus L.). J Med Plants Res, 4: 1187-1196.
  • Taverne YJ, Merkus D, Bogers AJ, Halliwell B, Duncker DJ, Lyons TW. 2018. Reactive Oxygen Species: Radical Factors in the Evolution of Animal Life: A molecular timescale from Earth's earliest history to the rise of complex life. BioEssays, 40: 1700158.
  • Twala PP, Mitema A, Baburam C, Feto NA. 2020. Breakthroughs in the discovery and use of different peroxidase isoforms of microbial origin. AIMS Microbiol, 6: 330.
  • Veitch NC. 2004. Horseradish peroxidase: a modern view of a classic enzyme. Phytochem, 65: 249-259.
  • Wakamatsu K, Takahama U. 1993. Changes in peroxidase activity and in peroxidase isozymes in carrot callus. Physiol Plant, 88: 167-171.
  • Welinder KG. 1992. Superfamily of plant, fungal and bacterial peroxidases. Curr Opin Struct Biol, 2: 388-393.
  • Wu CS, Gao QH, Guo XD, Yu JG, Wang M. 2012. Effect of ripening stage on physicochemical properties and antioxidant profiles of a promising table fruit ‘pear-jujube’(Zizyphus jujuba Mill.). Sci Hortic, 148: 177-184.
  • Zainudin MAM, Hamid AA, Anwar F, Osman A, Saari N. 2013. Variation of bioactive compounds and antioxidant activity among three cultivars (B2, B10 and B17) of carambola (Averrhoa carambola L.) fruits. Agrochimica, 57: 264-278.
  • Zainudin MAM, Hamid AA, Anwar F, Osman A, Saari N. 2014. Variation of bioactive compounds and antioxidant activity of carambola (Averrhoa carambola L.) fruit at different ripening stages. Sci Hortic, 172: 325-331.

Characterization of Guaiacol Peroxidase Enzyme from Carambola Fruit

Year 2023, , 275 - 280, 01.05.2023
https://doi.org/10.47115/bsagriculture.1269682

Abstract

Carambola is a fruit grown in tropical and subtropical regions of the world. Natural antioxidants including vitamin C, carotenoids, and certain phenolic substances are abundant in carambola fruit. As antioxidants support health by acting as nutraceutical and functional food additives, they help preserve food by preventing oxidation processes. The oxidation of various organic or inorganic substrates by hydrogen peroxide or organic peroxides as terminal oxidants is a process in which peroxidase, which is abundantly present in fruits and vegetables, participates. In this study, guaiacol peroxidase enzyme from carambola fruit was partially purified and characterized. Purification procedure made up the homogenate preparation, ammonium sulfate precipitation, and dialysis. After purification, optimum ionic strength, pH and substrate concentration were investigated. These values were determined as 200 mM Tris, pH: 7.5, 7.5 mM H2O2 and 15 mM guaiacol for carambola fruit guaiacol peroxidase enzyme, respectively.

References

  • Aghelan Z, Shariat SZS. 2015. Partial purification and biochemical characterization of peroxidase from rosemary (Rosmarinus officinalis L.) leaves. Adv Biomed Res, 4.
  • Al-Senaidy AM, Ismael MA. 2011. Purification and characterization of membrane-bound peroxidase from date palm leaves (Phoenix dactylifera L.). Saudi J Biol Sci, 18: 293-298.
  • Ashie INA, Simpson BK, Smith JP. 1996. Mechanisms for controlling enzymatic reactions in foods. Crit Rev Food Sci Nutr, 36: 1-30.
  • Barrett DM, Beaulieu JC, Shewfelt R 2010. Color, flavor, texture, and nutritional quality of fresh-cut fruits and vegetables: desirable levels, instrumental and sensory measurement, and the effects of processing. Crit Rev Food Sci Nutr, 50: 369-389.
  • Bursal E. 2013. Kinetic properties of peroxidase enzyme from chard (Beta vulgaris Subspecies cicla) leaves. Int J Food Prop, 16: 1293-1303.
  • Castrejón ADR, Eichholz I, Rohn S, Kroh LW, Huyskens-Keil S. 2008. Phenolic profile and antioxidant activity of highbush blueberry (Vaccinium corymbosum L.) during fruit maturation and ripening. Food Chem, 109: 564-572.
  • Dasgupta P, Chakraborty P, Bala NN. 2013. Averrhoa carambola: an updated review. Int J Pharm Res, 2: 54-63.
  • Erdem HÜ, Kalın R, Özdemir N, Özdemir H. 2015. Purification and biochemical characterization of peroxidase isolated from white cabbage (Brassica oleracea var. capitata f. alba). Int J Food Prop, 18: 2099-2109.
  • Franzini L, Ardigo D, Valtuena S, Pellegrini N, Del Rio D, Bianchi MA, Scazzina F, Piatti PM, Brighenti F, Zavaroni I. 2012. Food selection based on high total antioxidant capacity improves endothelial function in a low cardiovascular risk population. Nutr Metab Cardiovasc Dis, 22: 50-57.
  • Gelen V, Şengül E, Yıldırım S, Senturk E, Tekin S, Kükürt A. 2021. The protective effects of hesperidin and curcumin on 5-fluorouracil–induced nephrotoxicity in mice. Environ Sci Pollut Res, 28: 47046-47055.
  • Gupta S K, Sharma M, Deeba F, Pandey V. 2017. Role of reactive oxygen species in photophosphorylation and damage to D1 protein: past and present. Reactive Oxygen Species in Plants: Boon Or Bane‐Revisiting the Role of ROS, 165-186.
  • Herath N, Kodithuwakku G, Dissanayake T, Rathnathunga N, Weerakoon K. 2021. Acute Kidney Injury Following Star Fruit Ingestion: A Case Series. Wilderness Environ Med, 32: 98-101.
  • Hu Y, Wu J, Luo P, Mo Y. 2012. Purification and partial characterization of peroxidase from lettuce stems. Afr J Biotechnol, 11: 2752-2756.
  • Huang R, Xia R, Hu L, Lu Y, Wang M. 2007. Antioxidant activity and oxygen-scavenging system in orange pulp during fruit ripening and maturation. Sci Hortic, 113: 166-172.
  • Ikram EHK, Eng KH, Jalil AMM, Ismail A, Idris S, Azlan A, Nazri HSM, Diton NAM, Mokhtar, RAM. 2009. Antioxidant capacity and total phenolic content of Malaysian underutilized fruits. J Food Compos Anal, 22: 388-393.
  • Ilahy R, Hdider C, Lenucci MS, Tlili I, Dalessandro G. 2011. Antioxidant activity and bioactive compound changes during fruit ripening of high-lycopene tomato cultivars. J Food Compos Anal, 24: 588-595.
  • Isabelle M, Lee BL, Lim MT, Koh WP, Huang D, Ong CN. 2010. Antioxidant activity and profiles of common fruits in Singapore. Food Chem, 123: 77-84.
  • Koksal E, Bursal E, Aggul AG, Gulcin I. 2012. Purification and characterization of peroxidase from sweet gourd (Cucurbita Moschata Lam. Poiret). Int J Food Prop, 15: 1110-1119.
  • Lampila P, van Lieshout M, Gremmen B, Lähteenmäki L 2009. Consumer attitudes towards enhanced flavonoid content in fruit. Int Food Res J, 42: 122-129.
  • Lavery CB, MacInnis MC, MacDonald MJ, Williams JB, Spencer CA, Burke AA, Irwin DJG, D’Cunha GB. 2010. Purification of peroxidase from horseradish (Armoracia rusticana) roots. J Agric Food Chem, 58: 8471-8476.
  • Leong LP, Shui G. 2002. An investigation of antioxidant capacity of fruits in Singapore markets. Food Chem, 76: 69-75.
  • López-Serrano M, Barceló AR. 1996. Purification and characterization of a basic peroxidase isoenzyme from strawberries. Food Chem, 55: 133-137.
  • Loukili AE, Limam F, Ayadi A, Boyer N, Ouelhazi L. 1999. Purification and characterization of a neutral peroxidase induced by rubbing tomato internodes. Physiol Plant, 105: 24-31.
  • Maciel HPF, Gouvêa CMCP, Toyama M, Smolka M, Marangoni S, Pastore GM. 2007. Extraction, purification and biochemical characterization of a peroxidase from Copaifera langsdorffii leaves. Quim Nova, 30: 1067-1071.
  • Mafulul SG, Joel EB, Barde LA, Lenka JL, Ameh AA, Phililus MG. 2018. Extraction, partial purification and characterization of peroxidase from Calotropis procera leaves. J adv biol biotechnol, 18: 1-10.
  • Manda H, Vyas K, Pandya A, Singhal G. 2012. A complete review on: Averrhoa carambola. World J Pharm Pharm Sci, 1: 17-33.
  • Manu BT, Rao UP. 2009. Calcium modulated activity enhancement and thermal stability study of a cationic peroxidase purified from wheat bran. Food Chem, 114: 66-71.
  • Marnewick JL, Rautenbach F, Venter I, Neethling H, Blackhurst DM, Wolmarans P, Macharia M. 2011. Effects of rooibos (Aspalathus linearis) on oxidative stress and biochemical parameters in adults at risk for cardiovascular disease. J Ethnopharmacol, 133: 46-52.
  • Muthu N, Lee SY, Phua KK, Bhore SJ. 2016. Nutritional, medicinal and toxicological attributes of star-fruits (Averrhoa carambola L.): a review. Bioinformation, 12: 420.
  • O'Dougherty M, Harnack LJ, French SA, Story M, Oakes JM, Jeffery RW. 2006. Nutrition labeling and value size pricing at fast-food restaurants: a consumer perspective. Am J Health Promot, 20: 247-250.
  • Olson KR, Straub KD. 2015. The role of hydrogen sulfide in evolution and the evolution of hydrogen sulfide in metabolism and signaling. Physiology, 31: 60-72
  • Park YK, Park E, Kim JS, Kang MH. 2003. Daily grape juice consumption reduces oxidative DNA damage and plasma free radical levels in healthy Koreans. Mutat Res Fundam Mol Mech Mutagen, 529: 77-86.
  • Passardi F, Theiler G, Zamocky M, Cosio C, Rouhier N, Teixera F, Margis-Pinheiro M, Ioannidis V, Penel C, Falquet L, Dunand C. 2007. PeroxiBase: the peroxidase database. Phytochem, 68: 1605-1611.
  • Rautiainen S, Levitan EB, Mittleman MA, Wolk A. 2013. Total antioxidant capacity of diet and risk of heart failure: a population-based prospective cohort of women. Am J Med, 126: 494-500.
  • Sakharov IY, Castillo JL, Areza JC, Galaev IY. 2000. Purification and stability of peroxidase of African oil palm Elaies guineensis. Bioseparation, 9: 125-132.
  • Sengul E, Gelen V, Yildirim S, Senturk E, Dag Y, Eser G, Gok M. 2021. Investigation of effects of Silymarin in 5-fluorouracil hepatotoxicity and nephrotoxicity in Mice.
  • Sewelam N, Kazan K, Schenk PM. 2016. Global plant stress signaling: reactive oxygen species at the cross-road. Front Plant Sci, 7: 187.
  • Shofian NM, Hamid AA, Osman A, Saari N, Anwar F, Pak Dek MS, Hairuddin MR. 2011. Effect of freeze-drying on the antioxidant compounds and antioxidant activity of selected tropical fruits. Int J Mol Sci, 12: 4678-4692.
  • Shui G, Wong SP, Leong LP. 2004. Characterization of antioxidants and change of antioxidant levels during storage of Manilkara zapota L. J Agric Food Chem, 52: 7834-7841.
  • Smith IK, Vierheller TL, Thorne CA. 1988. Assay of glutathione reductase in crude tissue homogenates using 5, 5′-dithiobis (2-nitrobenzoic acid). Anal. Biochem, 175: 408-413.
  • Şişecioğlu M, Gülçin İ, Cankaya M, Atasever A, Şehitoğlu MH, Kaya HB, Özdemir H. 2010. Purification and characterization of peroxidase from Turkish black radish (Raphanus sativus L.). J Med Plants Res, 4: 1187-1196.
  • Taverne YJ, Merkus D, Bogers AJ, Halliwell B, Duncker DJ, Lyons TW. 2018. Reactive Oxygen Species: Radical Factors in the Evolution of Animal Life: A molecular timescale from Earth's earliest history to the rise of complex life. BioEssays, 40: 1700158.
  • Twala PP, Mitema A, Baburam C, Feto NA. 2020. Breakthroughs in the discovery and use of different peroxidase isoforms of microbial origin. AIMS Microbiol, 6: 330.
  • Veitch NC. 2004. Horseradish peroxidase: a modern view of a classic enzyme. Phytochem, 65: 249-259.
  • Wakamatsu K, Takahama U. 1993. Changes in peroxidase activity and in peroxidase isozymes in carrot callus. Physiol Plant, 88: 167-171.
  • Welinder KG. 1992. Superfamily of plant, fungal and bacterial peroxidases. Curr Opin Struct Biol, 2: 388-393.
  • Wu CS, Gao QH, Guo XD, Yu JG, Wang M. 2012. Effect of ripening stage on physicochemical properties and antioxidant profiles of a promising table fruit ‘pear-jujube’(Zizyphus jujuba Mill.). Sci Hortic, 148: 177-184.
  • Zainudin MAM, Hamid AA, Anwar F, Osman A, Saari N. 2013. Variation of bioactive compounds and antioxidant activity among three cultivars (B2, B10 and B17) of carambola (Averrhoa carambola L.) fruits. Agrochimica, 57: 264-278.
  • Zainudin MAM, Hamid AA, Anwar F, Osman A, Saari N. 2014. Variation of bioactive compounds and antioxidant activity of carambola (Averrhoa carambola L.) fruit at different ripening stages. Sci Hortic, 172: 325-331.
There are 49 citations in total.

Details

Primary Language English
Subjects Agricultural Engineering
Journal Section Research Articles
Authors

Ömer Taş 0000-0003-1782-8210

Maviş Yayla 0009-0007-3259-4404

Deniz Ekinci 0000-0001-7849-4117

Early Pub Date April 30, 2023
Publication Date May 1, 2023
Submission Date March 23, 2023
Acceptance Date April 18, 2023
Published in Issue Year 2023

Cite

APA Taş, Ö., Yayla, M., & Ekinci, D. (2023). Characterization of Guaiacol Peroxidase Enzyme from Carambola Fruit. Black Sea Journal of Agriculture, 6(3), 275-280. https://doi.org/10.47115/bsagriculture.1269682

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