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The Effect of Donors Used in In vivo Maternal Haploid Technique on Haploid Induction Rate

Year 2023, , 528 - 532, 01.09.2023
https://doi.org/10.47115/bsagriculture.1320610

Abstract

Maize is an important plant grown to obtain grain and silage, and is used in human and animal nutrition. In conventional maize breeding studies, inbred line development studies are carried out for at least 7 years if a single generation is obtained in a year, while it is possible to develop 100% homozygous lines in a short period of 2 years with the in vivo maternal haploid technique. The in vivo maternal haploid technique is widely used in advanced maize breeding programs. The choice of donor or source material to be used for haploid induction depends on the purpose of the breeding program. Generally, breeders use F1 or F2 populations as source material for haploid induction. In this study; 30 F1 genotypes and their F2s were crossed with the inducer line. The putative haploid seed was identified by considering the R1-nj color marker, and the haploid induction rate was determined. The effect of the generations of the donor genotypes on the haploid induction rate was compared by performing an independent sample test, and the haploid induction rate obtained from the F1 donors was found to be higher than the haploid induction rate of the F2 donors. It was determined that there was a change in the haploid induction rate as the genotypes changed within the F1 and F2 donor groups.

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Project Number

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Thanks

The MHI (Moldovian Haploid Inducer) inducer line used in this study was obtained from Maize Genetics and Genomics Database and I would like to thank Maize GDB.

References

  • Beckert M. 1994. Advantages and disadvantages of the use of in vitro/in situ produced DH maize plants. In: Y. P. S. Bajaj (ed.). Biotechnology in Agriculture and Forestry. Vol. 25. Maize. Springer Verlag, Berlin, pp: 201-213.
  • Chaikam V, Boddupalli MP. 2012. Doubled Haploid Technology in Maize Breeding: Theory and Practice. CIMMYT, Mexico, pp: 20-23.
  • Chalyk ST. 1994. Properties of maternal haploid maize plants and potential application to maize breeding. Euphytica, 79: 13-18.
  • Chase SS. 1969. Monoploids and monoploid derivates of maize (Zea mays L.). Bot Rev, 35: 117-167.
  • Coe EH, Sarkar KR. 1964. The detection of haploids in maize. J Heredity, 55: 231-233.
  • Eder J, Chalyk ST. 2002. In vivo haploid induction in maize. Theor. Appl Genet, 104: 703-708.
  • Geiger HH. 2009. Doubled Haploids. In: Bennetzen JL, Hake S (eds.). Maize Handbook-Volume II: Genetics and Genomics. Springer Verlag, New York, US, pp: 641-657.
  • Hu G. 2014. Study on haploid induction rates in different maize inducers. Agri Sci Tech, 15(4): 554-556.
  • Kahrıman F, Kahrıman A, Güz AM, Yüksel NN. 2022. Development of homozygous maize lines differing in oil and zein content using in-vivo maternal haploid technique. Biotech Stud, 31 (2), 79-86.
  • Prigge V, Sanchez C, Dhillon BS, Schipprack W, Araus JL, Banziger M, Melchinger AE. 2011. Doubled haploids in tropical maize: I. Effects of inducers and source germplasm on in vivo haploid induction rates. Crop Sci, 51: 1498-1506.
  • Röber FK, Gordillo GA, Geiger HH. 2005. In vivo haploid induction in maize-performance of new inducers and significance of doubled haploid lines in hybrid breeding. Maydica, 50: 275-283.
  • Roux SR. 1995. Züchterische Untersuchungen zur in-vivo-Haploideninduktion bei Mais. PhD. Thesis, University of Hohenheim, Stuttgart, Germany.
  • Russel WA, Eberhart SA. 1975. Hybrid performance of selected maize lines from reciprocal recurrent and testcross selection programs. Crop Sci, 15: 1-4.
  • Soylu S. 2022. Mısır Yetiştiriciliği. Mısır; Islah Teknikleri ve Yetiştiriciliği. İKSAD Yayınevi, Ankara, Türkiye, pp: 547-574.
  • Spitkó T, Sági LJ, Pintér LC, Barnabás MB. 2006. Haploid regeneration aptitude of maize (Zea mays L.) lines of various origin and of their hybrids. Maydica, 51: 537-542.
Year 2023, , 528 - 532, 01.09.2023
https://doi.org/10.47115/bsagriculture.1320610

Abstract

Project Number

--

References

  • Beckert M. 1994. Advantages and disadvantages of the use of in vitro/in situ produced DH maize plants. In: Y. P. S. Bajaj (ed.). Biotechnology in Agriculture and Forestry. Vol. 25. Maize. Springer Verlag, Berlin, pp: 201-213.
  • Chaikam V, Boddupalli MP. 2012. Doubled Haploid Technology in Maize Breeding: Theory and Practice. CIMMYT, Mexico, pp: 20-23.
  • Chalyk ST. 1994. Properties of maternal haploid maize plants and potential application to maize breeding. Euphytica, 79: 13-18.
  • Chase SS. 1969. Monoploids and monoploid derivates of maize (Zea mays L.). Bot Rev, 35: 117-167.
  • Coe EH, Sarkar KR. 1964. The detection of haploids in maize. J Heredity, 55: 231-233.
  • Eder J, Chalyk ST. 2002. In vivo haploid induction in maize. Theor. Appl Genet, 104: 703-708.
  • Geiger HH. 2009. Doubled Haploids. In: Bennetzen JL, Hake S (eds.). Maize Handbook-Volume II: Genetics and Genomics. Springer Verlag, New York, US, pp: 641-657.
  • Hu G. 2014. Study on haploid induction rates in different maize inducers. Agri Sci Tech, 15(4): 554-556.
  • Kahrıman F, Kahrıman A, Güz AM, Yüksel NN. 2022. Development of homozygous maize lines differing in oil and zein content using in-vivo maternal haploid technique. Biotech Stud, 31 (2), 79-86.
  • Prigge V, Sanchez C, Dhillon BS, Schipprack W, Araus JL, Banziger M, Melchinger AE. 2011. Doubled haploids in tropical maize: I. Effects of inducers and source germplasm on in vivo haploid induction rates. Crop Sci, 51: 1498-1506.
  • Röber FK, Gordillo GA, Geiger HH. 2005. In vivo haploid induction in maize-performance of new inducers and significance of doubled haploid lines in hybrid breeding. Maydica, 50: 275-283.
  • Roux SR. 1995. Züchterische Untersuchungen zur in-vivo-Haploideninduktion bei Mais. PhD. Thesis, University of Hohenheim, Stuttgart, Germany.
  • Russel WA, Eberhart SA. 1975. Hybrid performance of selected maize lines from reciprocal recurrent and testcross selection programs. Crop Sci, 15: 1-4.
  • Soylu S. 2022. Mısır Yetiştiriciliği. Mısır; Islah Teknikleri ve Yetiştiriciliği. İKSAD Yayınevi, Ankara, Türkiye, pp: 547-574.
  • Spitkó T, Sági LJ, Pintér LC, Barnabás MB. 2006. Haploid regeneration aptitude of maize (Zea mays L.) lines of various origin and of their hybrids. Maydica, 51: 537-542.
There are 15 citations in total.

Details

Primary Language English
Subjects Agricultural Engineering (Other)
Journal Section Research Articles
Authors

Rahime Cengiz 0000-0001-6355-7496

Project Number --
Publication Date September 1, 2023
Submission Date June 27, 2023
Acceptance Date August 10, 2023
Published in Issue Year 2023

Cite

APA Cengiz, R. (2023). The Effect of Donors Used in In vivo Maternal Haploid Technique on Haploid Induction Rate. Black Sea Journal of Agriculture, 6(5), 528-532. https://doi.org/10.47115/bsagriculture.1320610

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