The Usability of Extracts of Walnut Green Outer Shell for In vitro Surface Sterilization of Rosehip (Rosa canina L.) Plant

Year 2023, , 631 - 635, 01.11.2023

Ebru Şirin , Bahadır Altun , Ahmet Kazankaya

https://doi.org/10.47115/bsagriculture.1353925

Cited By: 1

Abstract

In this study, it was aimed to determine the efficiency ratios of extracts obtained from walnut outer green shell at different concentrations in order to provide surface sterilization in in vitro propagation of rosehip plant. Axillary buds taken in June were used as explants. After surface sterilization of the explants with walnut outer shell extract, they were cultured in MS0 nutrient medium. The lowest contamination rate on the 5th and 7th days was observed in the control and 20K groups. In addition, the highest number of shoots per explant was obtained in the 20K group. Uncontaminated explants were subcultured to MS0 medium containing 1.0 mg /L IBA + 1.0 mg /L BAP and 1.0 mg/L BAP + 1.0 mg/L NAA at the end of the 7th day. Rooting was not observed in the explants due to browning. In the in vitro propagation of plants, extract (20K) obtained from oven-dried walnut shell can be used instead of chemical sterilant.

Keywords

Rosa canina L., Walnut shell extract, Tissue culture, Antimicrobial activity, Antifungal activity

References

• Adebomojo AA, AbdulRahaman AA. 2020. Surface sterilization of Ocimum seeds and tissues with biosynthesized nanosilver and its effects on callus induction. IOP Conf. Series: Mater Sci Eng, 805: 012024.
• Ambros EV, Vasilyeva OY, Novikova T. 2016. Effects of in vitro propagation on ontogeny of Rosa canina L. micropropagated plants as a promising rootstock for ornamental roses. Plant Cell Biotechnol Mol Biol, 17: 72-78.
• Babaoğlu M, Gürel E, Özcan S. 2002. Bitki Biyoteknolojisi-I, Doku Kültürü ve Uygulamaları. Selçuk Üniversitesi YAyınları, Konya, Türkiye, pp: 374.
• Balaj N, Zogaj R. 2011. Production seedlings of roses by grafting with bud for hybrid teas and climbing roses cultivars. Res J Agri Sci, 43: 155-160.
• Bhat MS. 1992. Micropropagation in rose. Indian Horticult, 37: 17-19.
• Bressan RH, Kim YJ, Hyndman SE, Hasegawa PM, Bressan RA. 1982. Factors affecting in vitro propagation of rose. J Amer Soc Hort Sci, 107: 979-990
• Canli FA, Kazaz S. 2009. Biotechnology of roses progress and future prospects. Suleyman Demirel Univ Orman Fak Derg, 1: 167-183.
• Cassells AC. 1991. Problems in tissue culture: Culture contamination. In: Micropropagation Technology tind Application. P.C. Debergh and R.H. Zimmerman, eds. Kluwer Academic Publishers, Dordrecht, Netherlands, pp: 31-44.
• Clark AM, Jurgens TM, Hufford CD. 1990. Antimicrobial activity of juglone. Phytotherapy Res, 4(1): 11-14.
• Cosmulescu SN, Trdafir I, Achim G, Baciu A. 2011. Juglone content in leaf and green husk of five walnut (Juglans regia L.) cultivars. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 39(1): 237-240.
• Daglish C. 1950. The determination and occurrence of hydrojuglone glucoside in the walnut. Biochem J, 47: 458-462.
• Dama LB, Poul BN, Jadhav BV. 1998. Antimicrobial activity of naphthoquinonic compounds. J Ecotoxicol Environ Monit, 8(3): 213-215.
• Davis EF. 1928. The toxic principle of Juglans nigra as identifiedwith synthetic juglone and its toxic effects on tomato and alfalfaplants. Am J Bot, 15: 620.
• Genç S, Soysal Mİ. 2018. Parametric and nonparametric post hoc tests. BSJ Eng Sci, 1(1): 18-27.
• Horn W. 1992. Micropropagation of rose (Rosa L.). Biotechnol Agric, 20: 320342.
• Kavand S, Jafarkhani Kermani JM, Haghnazari A, Khosravi P, Azimi MR. 2011. Micropropagation and medium-term conservation of Rosa pulverulenta. Acta Scientiarum Agron, 33(2): 297-301.
• Kazaz S, Baydar H, Erbas S. 2009. Variations in chemical compositions of Rosa damascena Mill. and Rosa canina L. fruits. Czech J Food Sci, 27: 178-184.
• Khorrami S, Zarrabi A, Khaleghi M, Danaei M, Mozafari M. 2018. Selective cytotoxicity of green synthesized silver nanoparticles against the MCF-7 tumor cell line and their enhanced antioxidant and antimicrobial properties. Int J Nanomedic, 13: 8013-8024.
• Khosh-Khui M, Sink KC. 1982. Callus induction and culture of Rosa. Sci Hortic, 17: 361- 370.
• Kocaçalışkan I, Terzi I. 2001. Allelopathic effects of walnut leaf extracts and juglone on seed germination and seedling growth. J Horticult Sci Biotechnol, 76(4): 436-440.
• Marković M, Đunisijević-Bojović D, Skočajić D, Milutinović M, Buvač K. 2021. Optimizing The Micropropagation Protocol for Rosa canina L. Elite Genotype Propagation in The Belgrade Area. Glasnik Sumarskog Fakulteta, 123: 87-96
• Mederos S, Rodríguez Enríquez MJ. 1987. In vitro propagation of “Golden Times” roses. Factors affecting shoot tips and axillary bud growth and morphogenesis. Acta Hortic, 212: 619-624.
• Ngezahayo F, Liu B. 2014. Axillary bud proliferation approach for plant biodiversity conservation and restoration. Int J Biodiver, 2014: 1-9.
• Pahnekolayi DV, Tehranifar A, Samiei L, Shoor M. 2014. Micropropagation of Rosa canina through axillary shoot proliferation. J Ornament Plants, 4(1): 45-51.
• Pati PK, Rath SP, Sharma M, Sood A, Ahuja PS. 2006. In vitro propagation of rose-a review. Biotechnol Advan, 24: 94-114.
• Pawłowski T, Bujarska-Borkowska B, Suszka J, Tylkowski T, Chmielarz P, Klupczyńska E, Staszak A. 2020. Temperature regulation of primary and secondary seed dormancy in Rosa canina L.: Findings from proteomic analysis. Int J Mol Sci, 21: 7008.
• Pereira JA, Olivera I, Sousa A, Ferreira ICFR, Bento A, Estenvinho L. 2008. Bioactivite properties and chemical composition of six walnut (Juglans regia L.) cultivars. Food Chem Toxicol, 46: 2103-2011.
• Prataviera AG, Kuniyuki AH, Ryugo K. 1983. Growth inhibitors in xylem exudates of Persian walnuts (Juglans regia L.) and their possible role in graft failure. J American Soc Horticult Sci, 108: 1043-1045.
• Rice EL. 1984. Allelopathy. Academic Press, Orlando, Florida, US.
• Robberts AV, Schum A. 2003. Cell tissue and organ culture. In: Roberts, A. V., Debener, T. and Gudin, S. (Ed). Encyclopedia of rose science. Elsevior Academic Press, Oxford, UK, pp: 57-110.
• Shirdel M, Motallebi-Azar A, Matloobi M, Zaare-Nahandi F. 2013. Effects of nodal position and growth regulators on in vitro growth of dog rose (Rosa canina). J Ornament Horticult Plants, 3: 9-17.
• Stampar F, Solar A, Hudina M, Veberic R, Colaric M. 2006. Traditional walnut liqueur cocktail of phenolics. Food Chem, 95(4): 627-631.
• Tan DTC, Osman H, Mohamad S, Kamaruddin AH. 2012. Synthesis and antibacterial activity of juglone derivatives. J Chem Eng, 6(1): 84-89.
• Van der Mark F, Pijnacker-Hordijk PJ, Varega GAI De Vries DP, Dons JJM. 1990. In vivo transformation of clonal Rosa canina rootstocks with Agrobacterium rhizogenes. J Genet Breeding, 44: 263-268.
• Vijaya N, Satyanarayana G, Prakash J, Pierik RLM. 1991. Effect of culture media and growth regulators on in vitro propagation of rose. Curr Plant Sci Biotechnol Agri,12: 209-214.
• Wegg SM, Townsley PM. 1983. Ascorbic acid in cultured tissue of briar rose, Rosa rugosa Thunb. Plant Cell Rep, 2: 78-81.
• Zapata C, Srivatanakul M, Park SH, Lee BM, Salas MG, Smith H. 1999. Improvements in shoot apex regeneration of two fiber crops: Cothon and Kenaf. Plant Cell Tissue Organ Cult, 54: 185-191.