DGAT1 Gene Polymorphism in Morkaraman and Tushin

Year 2024, , 45 - 50, 01.01.2024

İremnur Aydın , Sinan Kopuzlu

https://doi.org/10.47115/bsagriculture.1388068

Abstract

This study aims to investigate the polymorphism of the Diacylglycerol acyltransferase1 (DGAT1) gene locus in 105 Morkaraman and 65 Tushin lambs to determine the distribution of genotype and allele frequencies of lambs in terms of related genes. DGAT1/Alu1 gene polymorphism was defined by using the PCR-RFLP method in the DNAs isolated from hair samples taken from Morkaraman and Tushin lambs used in this study. PCR- RFLP products were run in an electrophoresis medium and the results were visualized on an ultraviolet (UV) transluminator. When the population was examined in terms of allele frequencies, it was defined that the C allele and the T allele were 0.72% and 0.28% for the Morkaraman, and 0.71% and 0.29% for Tushin, respectively. The CC, CT, and TT genotype frequencies of the DGAT1 gene in the population were found to be 53.3%, 38.1%, and 8.6% for the Morkaraman and 50.8%, 40.0%, and 9.2% for the Tushin, respectively. In the Hardy-Weinberg genetic equilibrium test, it was observed that the distribution of genotype frequencies was in balance (P>0.05) in the population. It has been defined that the genotype and allele frequencies determined in terms of DGAT1 gene polymorphism may be found to be sufficient to reveal the genotype diversity of the breeds. The genotype and allele frequencies determined in terms of DGAT1 gene polymorphism were sufficient to reveal the genotype diversity of the breed, the sheep with CC genotype are economically advantageous in the herd, and therefore DGAT1 gene can be used for marker-assisted selection (MAS).

Keywords

Polymorphism, DGAT1 gene, PCR-RFLP method, Sheep

Ethical Statement

Kıl örneklerinden çalışma olduğu için Fakülte Etik Kurulundan belgelendirilmiştir.

Supporting Institution

Atatürk Üniversitesi BİLİMSEL ARAŞTIRMA PROJELERİ KOORDİNASYON BİRİMİ

Project Number

FYL-2021-9187

Thanks

As the authors, we would like to thank the Atatürk University Scientific Research Projects Coordination Unit (Project number: FYL-2021-9187) for supporting the study.

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