Year 2018, Volume 1 , Issue 2, Pages 34 - 37 2018-04-01

Investigation of the Antifungal Effect of Thymbra Spicata L. and Rosmarinus Officinalis L. Essential Oils on Fusarium Oxysporum F.Sp. Cucumerinum and Fusarium Oxysporum F.Sp. Melonis

Yusuf BAYAN [1]


This study was carried out to determine the antifungal activity of Thymbra spicata L. and Rosmarinus officinalis L. plant essential oils. In this context, the antifungal effect of 0 (Control), 0.5, 1, 2, 4, 8 µlpetri-1 doses of plant essential oils on Fusarium oxysporum f. sp. cucumerinum (FOC) and Fusarium oxysporum f. sp. melonis (FOM) was identified. In order to determine the antifungal effect of essential oils, the filter paper adhered to the covers of the petri dishes was impregnated with the micropipette. Additionally, it was suggested that the plant essential oils inhibited the mycelial growth of FOM and FOC compared to the control. As a result of the study, 8 µlpetri-1 dose of T. spicata essential oil inhibited the mycelium growth of FOC and FOM by 100%. On the other hand, it was determined that 8 µlpetri-1 dose of R. officinalis essential oil inhibited the mycelium growth of FOC and FOM by 61.32% and 71.97%, respectively. In the study, LC50 and LC90 values were also calculated by carrying out a dose-effect study. The LC50 and LC90 values of FOM and FOC for T. spicata’nın essential oil were calculated as 0.58-1.26 µL and 0.46-1.07 µL, respectively, while the LC50 and LC90 values, of FOM and FOC for R. officinalis essential oil were calculated as 4.04-37.56 µL and 3.92-95.84 µL respectively. The T spicata, R. officinalis essential oils used in the experiment have a potential to be an alternative to fungus in controlling FOC and FOM diseases. It was concluded that the essential oils used in the experiment have a potential to be an alternative to synthetic pesticides in controlling diseases.

Antifungal effect, Thymbra spicata, Rosmarinus officinalis, Essential oil
  • Baruah P, Sharma RK, Singh RS, Ghosh AC. 1996. Fungicidal Activity of Some Naturally Occurring Essential Oils Against Fusarium moniliforme. J Essen Oil Res, 8: 411-412.
  • Bayan Y, Genc N, Kusek M, Gul F, Imecik Z. 2017. Determinatıon of Chemical Compositions, Antifungal, Antibacterıal and Antioxidant Activity of Thymbra Spicata L. From Turkey. Fresenius Environ Bull, 26: 7595-7599.
  • Baytop T. 1984. Türkiye’de bitkilerle tedavi. İstanbul Üniversitesi Yayın No: 3255, Ecz. Fak. Yayın No:40, İstanbul.
  • Centeno S, Calvo MA. Adelantado C, Figueroa S. 2010. Antifungal activity of extracts of Rosmarinus officinalis and Thymus vulgaris against Aspergillus flavus and A. ochraceus. Pakistan J Biol Sci, 13 (9): 452-455.
  • Erzurum K, Taner Y, Secer E, Yanmaz R, Maden S. 1999. Occurrence of races of Fusarium oxysporum f.sp. melonis causing wilt on melon in Central Anatolia. J Turk Phytopathol, 28: 87-97.
  • Jenkins SF, Wehner TC. 1983. Occurrence of Fusarium oxysporum f. sp. cucumerinum on greenhouse-grown Cucumis sativus seed stocks in North Carolina. Plant Dis, 67: 1024-1025.
  • Kilic T. 2006. Analysis of Essential Oil Composition of Thymbra spicata var. spicata Antifungal, Antibacterial and Antimycobacterial Activities. Z. Naturforsch. 61, 324-328.
  • Lee SO, Choi GJ, Jang KS, Lim HK, Cho KY, Kim JC. 2007. Antifungal activity of five plant essential oils as fumigant against post-harvest and soilborne plant pathogenic fungi. Plant Pathol J, 23: 97-102.
  • Lopez-Reyes J G, Spadaro D, Gullino M L, Garibaldi A. 2010. Efficacy of plant essential oils on postharvest control of rot caused by fungi on four cultivars of apples in vivo. Flavour Fragrance J, 25: 171-177.
  • Mares D, Tosi B, Poli F, Andressential OC. 2004. Antifungal activity of Tagetes patula extracts on some phytopathogenic fungi: Ultrastructural evidence on Pythium ultimum. Microbiol Res, 159: 259-304.
  • Muller-Riebau FJ, Berger BM, Yegen O, Cakir C. 1997. Seasonal Variations in the Chemical Compositions of Essential Oils of Selected Aromatic Plants Growing Wild in Turkey. J Agric Food Chem, 45: 4821-4825.
  • Ozan S, Aşkın A. 2006. Örtü altı sebze alanlarında görülen fungal hastalıklar üzerine çalışmalar. Bitki Koruma Bülteni. 46(1-4), 65-75. Ozcan MS, Chalchat JC. 2008. Chemical composition and antifungal activity of rosemary (Rosmarinus officinalis L.) oil from Turkey. Inter J Food Sci Nutri, 59(7-8): 691-698.
  • Önen H, Özer Z., Telci I. 2002. Bioherbicidal effects of some plant essential oils on different weed species. Journal of Plant Diseases and Protection. Sonderheft XVIII. 349 597-605. ISSN 0938- 9938.
  • Pandey DK, Tripathi NN, Tripathi RD, Dixit SN, 1982. Fungitoxic and phytotoxic properties of essential oil of Hyptis suaveolens. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz, 89: 344- 349.
  • Schreuder W, Lamprecht SC, Holz G. 2000. Race determination and vegetative compatibility grouping of Fusarium oxysporum f. sp. melonis from South Africa. Plant Dis, 84: 231-234.
  • Sharma N, Verma UK. 2004. Bioactivity of Hyptis suaveolens on storage mycoflora. In 'International Conference on Fumigation and Controlled Atmosphere'. Conrad Jupiters, Gold coast, Australia, p. 55.
  • Soylu E M, Kurt S, Soylu S. 2010. In vitro and in vivo antifungal activities of the essential oils of various plants against tomato grey mould disease agent Botrytis cinerea. Int. J Food Microbiol, 143: 183-189.
  • Telci, İ., Bayram, E., Yılmaz, G., Avcı, B. 2006. Variability in essential oil composition of Turkish basils (Ocimum basilicum L.). Biochem System Ecol, 34: 489-497.
  • Vakalounakis DJ. 1996. Root and stem rot of cucumber caused by Fusarium oxysporum f.sp. radicis-cucumerinum, Plant Dis, 80: 313-316.
  • Yıldız M, Delen N. 1977. Studies on the Occurrence of Fusarium Wilt of Cucumber in Ege Region of Turkey. J Turk Phytopath, 6 (3): 111-117.
  • Yilar M, Bayan Y, Aksit H, Onaran A, Kadioglu I, Yanar Y. 2013. Bioherbicidal Effects of Essential Oils Isolated from Thymus fallax, Mentha dumetorum Schult. and Origanum vulgare L. Asian J Chem, 25, 4807-4811.
  • Yucel S, Pala H, Sarı N, Abak K. 1994. Determination of Fusarium oxysporum f.sp. melonis races in the East Mediterranean Region of Turkey and response of some melon genotypes to the disease. Proc. 9th Congr. Mediterranean Phytopathological Union. Turkish Phytopathol Soc Pub, 7: 87-89.
  • Yücel S. 1994. Survey studies of fungal diseases of protected vegetable areas in Mediterranean region. Bitki Koruma Bülteni, 34: (1-2) 23-34.
Primary Language en
Subjects Agricultural, Engineering
Journal Section Research Articles
Authors

Author: Yusuf BAYAN (Primary Author)
Institution: Kırşehir Ahi Evran Üniversitesi
Country: Turkey


Dates

Publication Date : April 1, 2018

Bibtex @research article { bsagriculture446991, journal = {Black Sea Journal of Agriculture}, issn = {}, eissn = {2618-6578}, address = {bsjagri@blackseapublishers.com}, publisher = {Hasan ÖNDER}, year = {2018}, volume = {1}, pages = {34 - 37}, doi = {}, title = {Investigation of the Antifungal Effect of Thymbra Spicata L. and Rosmarinus Officinalis L. Essential Oils on Fusarium Oxysporum F.Sp. Cucumerinum and Fusarium Oxysporum F.Sp. Melonis}, key = {cite}, author = {Bayan, Yusuf} }
APA Bayan, Y . (2018). Investigation of the Antifungal Effect of Thymbra Spicata L. and Rosmarinus Officinalis L. Essential Oils on Fusarium Oxysporum F.Sp. Cucumerinum and Fusarium Oxysporum F.Sp. Melonis . Black Sea Journal of Agriculture , 1 (2) , 34-37 . Retrieved from https://dergipark.org.tr/en/pub/bsagriculture/issue/38528/446991
MLA Bayan, Y . "Investigation of the Antifungal Effect of Thymbra Spicata L. and Rosmarinus Officinalis L. Essential Oils on Fusarium Oxysporum F.Sp. Cucumerinum and Fusarium Oxysporum F.Sp. Melonis" . Black Sea Journal of Agriculture 1 (2018 ): 34-37 <https://dergipark.org.tr/en/pub/bsagriculture/issue/38528/446991>
Chicago Bayan, Y . "Investigation of the Antifungal Effect of Thymbra Spicata L. and Rosmarinus Officinalis L. Essential Oils on Fusarium Oxysporum F.Sp. Cucumerinum and Fusarium Oxysporum F.Sp. Melonis". Black Sea Journal of Agriculture 1 (2018 ): 34-37
RIS TY - JOUR T1 - Investigation of the Antifungal Effect of Thymbra Spicata L. and Rosmarinus Officinalis L. Essential Oils on Fusarium Oxysporum F.Sp. Cucumerinum and Fusarium Oxysporum F.Sp. Melonis AU - Yusuf Bayan Y1 - 2018 PY - 2018 N1 - DO - T2 - Black Sea Journal of Agriculture JF - Journal JO - JOR SP - 34 EP - 37 VL - 1 IS - 2 SN - -2618-6578 M3 - UR - Y2 - 2020 ER -
EndNote %0 Black Sea Journal of Agriculture Investigation of the Antifungal Effect of Thymbra Spicata L. and Rosmarinus Officinalis L. Essential Oils on Fusarium Oxysporum F.Sp. Cucumerinum and Fusarium Oxysporum F.Sp. Melonis %A Yusuf Bayan %T Investigation of the Antifungal Effect of Thymbra Spicata L. and Rosmarinus Officinalis L. Essential Oils on Fusarium Oxysporum F.Sp. Cucumerinum and Fusarium Oxysporum F.Sp. Melonis %D 2018 %J Black Sea Journal of Agriculture %P -2618-6578 %V 1 %N 2 %R %U
ISNAD Bayan, Yusuf . "Investigation of the Antifungal Effect of Thymbra Spicata L. and Rosmarinus Officinalis L. Essential Oils on Fusarium Oxysporum F.Sp. Cucumerinum and Fusarium Oxysporum F.Sp. Melonis". Black Sea Journal of Agriculture 1 / 2 (April 2018): 34-37 .
AMA Bayan Y . Investigation of the Antifungal Effect of Thymbra Spicata L. and Rosmarinus Officinalis L. Essential Oils on Fusarium Oxysporum F.Sp. Cucumerinum and Fusarium Oxysporum F.Sp. Melonis. BSJ Agri.. 2018; 1(2): 34-37.
Vancouver Bayan Y . Investigation of the Antifungal Effect of Thymbra Spicata L. and Rosmarinus Officinalis L. Essential Oils on Fusarium Oxysporum F.Sp. Cucumerinum and Fusarium Oxysporum F.Sp. Melonis. Black Sea Journal of Agriculture. 2018; 1(2): 34-37.