Investigation of the Antifungal Effect of Thymbra Spicata L. and Rosmarinus Officinalis L. Essential Oils on Fusarium Oxysporum F.Sp. Cucumerinum and Fusarium Oxysporum F.Sp. Melonis

Year 2018, Volume: 1 Issue: 2, 34 - 37, 01.04.2018

Yusuf Bayan

Abstract

This
study was carried out to determine the antifungal activity of Thymbra spicata L. and Rosmarinus
officinalis L. plant essential oils. In this context, the antifungal
effect of 0 (Control), 0.5, 1, 2, 4, 8 µlpetri^-1 doses of plant
essential oils on Fusarium oxysporum f.
sp. cucumerinum (FOC) and Fusarium oxysporum f. sp. melonis (FOM) was identified. In order to determine the
antifungal effect of essential oils, the filter paper adhered to the covers of
the petri dishes was impregnated with the micropipette. Additionally, it was
suggested that the plant essential oils inhibited the mycelial growth of FOM
and FOC compared to the control. As a result of the study, 8 µlpetri^-1
dose of T. spicata essential oil inhibited
the mycelium growth of FOC and FOM by 100%. On the other hand, it was determined that 8 µlpetri^-1
dose of R. officinalis essential oil inhibited the
mycelium growth of FOC and FOM by 61.32% and 71.97%, respectively. In the
study, LC₅₀ and LC₉₀ values were also calculated by
carrying out a dose-effect study. The LC₅₀ and LC₉₀
values of FOM and FOC for T. spicata’nın essential oil were calculated as
0.58-1.26 µL and 0.46-1.07 µL, respectively, while the LC₅₀ and LC₉₀
values, of FOM and FOC for R. officinalis essential oil
were calculated as 4.04-37.56 µL and 3.92-95.84 µL respectively. The T spicata, R. officinalis
essential oils used in the experiment have a potential to be an alternative to fungus
in controlling FOC and FOM diseases. It was concluded that the essential oils
used in the experiment have a potential to be an alternative to synthetic
pesticides in controlling diseases.

Keywords

Antifungal effect, Thymbra spicata, Rosmarinus officinalis, Essential oil

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