In vitro Micropropagation of Maintainer White Head Cabbage Lines Using Cotyledon And Hypocotyl Explants

Year 2022, Volume: 5 Issue: 3, 180 - 188, 01.07.2022

Senay Murat Doğru Ahmet Balkaya Ertan Sait Kurtar

https://doi.org/10.47115/bsagriculture.1088157

Cited By: 1

Abstract

Brassica species are the most widely cultivated vegetable crops and improvement programs started in the last thirty years in Türkiye. Hybrid seed production is very difficult for Brassica vegetable species. Because the production of a new F1 hybrid cultivar needs a male sterile line (A), maintainer line (B), and also a male line (C). Biotechnological methods provide an excellent opportunity for new F1 hybrid cultivar improvement via in vitro maintenance of the breeding lines. Thus, in vitro propagation possibilities of Matsunami F1 cultivar and 3 white head cabbage maintainer inbred lines were examined using hypocotyl and cotyledon explants. The most successful results were obtained from MS + 2 mg/l and 4 mg/l BAP combinations. Matsunami F1 was prominent in terms of proliferation rate. Root formation was found to be considerably high in all genotypes. The use of in vitro propagation techniques is expected to provide significant benefits in head cabbage breeding programs.

Keywords

Cabbage breeding, Explant, in vitro regeneration, Maintainer lines

Thanks

The author thanks the Republic of Turkey Ministry of Agriculture and Forestry General Directorate of Agricultural Research and Policies (TAGEM) for their valuable support.

References

• Ahmad N, Anis M. 2005. In vitro mass propagation of Cucumis sativus L. from nodal segments. Turkish J Botany, 29(3): 237-240.
• Balkaya A, Yanmaz R, Apaydin A, Kar H. 2005. Morphological characterization of white head cabbage (Brassica oleracea var. capitata subvar. alba) genotypes in Turkey. New Zealand J Crop and Hort Sci, 33(4): 333-341.
• Basak H, Biswas BK, Azad MAK, Arifuzzaman M. 2012. Micropropagation of mustard (Brassica spp.) from leaf explants. Thai J Agri Sci, 45(2): 75-81.
• Bhatia R, Sundar Dey S, Sharma K, Parkash C, Kumar R. 2015. In vitro maintenance of CMS lines of Indian cauliflower: an alternative for conventional CMS-based hybrid seed production. J Hort Sci Biotechnol, 90(2): 171-179.
• Cristea TO, Ambarus S, Stoian L, Falticeanu M, Calin M. 2009. Studies regarding the establishment of a viable micropropagation and acclimatization protocol for Brassica oleracea L. plants obtained from in vitro culture. Acta Hort, 812: 343-348.
• Dai XG, Shi XP, Ye YM, Fu Q, Bao MZ. 2009. High frequency plant regeneration from cotyledon and hypocotyl explants of ornamental kale. Biol Plantarum, 53(4): 769-773.
• Ertaş F, Tuncer B. 2016. In vitro shoot and root regeneration in Ercis cabbage (Brassica oleraceae var. capitata). In VII International Scientific Agriculture Symposium," Agrosym 2016", 6-9 October 2016, Jahorina, Bosnia and Herzegovina. Proceedings, pp: 170-175.
• Gambhir G, Kumar P, Srivastava DK. 2017. High frequency regeneration of plants from cotyledon and hypocotyl cultures in Brassica oleracea cv. Pride of India. Biotechnol Rep, 15: 107-113.
• Genç S, Soysal Mİ. 2018. Parametric and nonparametric post hoc tests. BSJ Eng Sci, 1(1): 18-27.
• Gerszberg A, Hnatuszko-Konka K, Kowalczyk T. 2015. In vitro regeneration of eight cultivars of Brassica oleracea var. capitata. In vitro Cell Devel Biol Plant, 51(1): 80-87.
• Kar H, Karaağac O. 2016. Türkiye’de baş lahana ıslahı. Türkiye Tohumcular Bir Derg, 5(20): 24-29.
• Kieffer M, Simkins N, Fuller MP, Jellings AJ. 2001. A cost effective protocol for in vitro mass propagation of cauliflower. Plant Sci, 160(5): 1015-1024.
• Kumar P, Srivastava DK. 2015. High frequency organogenesis in hypocotyl, cotyledon, leaf and petiole explants of broccoli (Brassica oleracea L. var. italica), an important vegetable crop. Physiol Mol Biol Plants, 21(2): 279-285.
• Kurtar ES, Balkaya A, Kandemir D. 2020. A productive direct regeneration protocol for a wide range of winter squash (Cucurbita maxima Duch.) and pumpkin (Cucurbita moschata Duch.) lines. Romanian Biotechnol Lett, 25(2): 1348-1355.
• Munshi MK, Roy PK, Kabir MH, Ahmed G. 2007. In vitro regeneration of cabbage (Brassica oleracea L. var. capitata) through hypocotyl and cotyledon culture. Plant Tissue Cult Biotechnol, 17(2): 131-136.
• Murat Dogru S, Balkaya A, Kurtar ES. 2017. A comprehensive perspective on Brassica vegetable crops grown in Turkey. Acta Horticulturae VII International Symposium on Brassicas 1202, May 31, 2018, Pontevedra, Spain, pp. 1-8.
• Murata M, Orton TJ. 1987. Callus initiation and regeneration capacities in Brassica species. Plant Cell, Tissue Organ Cult, 11(2): 111-123.
• Pal SP, Alam I, Anisuzzaman M, Sarker KK, Sharmin SA, Alam MF. 2007. Indirect organogenesis in summer squash (Cucurbita pepo L.). Turkish J Agri Forest, 31(1): 63-70.
• Pant B, Manandhar S. 2007. In vitro propagation of carrot (Daucus carota L.). Scientific World, 5(5): 51-53.
• Pavlovic S, Vinterhalter B, Mitic N, Adzic S, Nenad P, Milan Z, Dragan V. 2010. In vitro shoot regeneration from seedling explants in Brassica vegetables: red cabbage, broccoli, savoy cabbage and cauliflower. Arch Biol Sci, 62(2): 337-345.
• Qamar Z, Jahangir GZ, Nasir IA, Husnain T. 2014. In vitro production of cabbage and cauliflower. Advanc Life Sci, 1(2): 112-118.
• Ravanfar SA, Aziz MA, Rashid AA, Shahida S. 2014. In vitro adventitious shoot regeneration from cotyledon explant of Brassica oleracea subsp. italica and Brassica oleracea subsp. capitata using TDZ and NAA. Pakistan J Botany, 46(1): 329-335.
• Sretenovic-Rajicic T, Mijatovic M, Vinterhalter D. 2002. In vitro culture as a tool for improvement of cabbage cultivars in Yugoslavia. In II Balkan Symposium on Vegetables and Potatoes, Acta Horticulture 579, May 31, 2002, Thessaloniki, Greece, pp. 209-213.