DGAT1 Gene Polymorphism in Morkaraman and Tushin

Year 2024, Volume: 7 Issue: 1, 45 - 50, 01.01.2024

İremnur Aydın , Sinan Kopuzlu

https://doi.org/10.47115/bsagriculture.1388068

Abstract

This study aims to investigate the polymorphism of the Diacylglycerol acyltransferase1 (DGAT1) gene locus in 105 Morkaraman and 65 Tushin lambs to determine the distribution of genotype and allele frequencies of lambs in terms of related genes. DGAT1/Alu1 gene polymorphism was defined by using the PCR-RFLP method in the DNAs isolated from hair samples taken from Morkaraman and Tushin lambs used in this study. PCR- RFLP products were run in an electrophoresis medium and the results were visualized on an ultraviolet (UV) transluminator. When the population was examined in terms of allele frequencies, it was defined that the C allele and the T allele were 0.72% and 0.28% for the Morkaraman, and 0.71% and 0.29% for Tushin, respectively. The CC, CT, and TT genotype frequencies of the DGAT1 gene in the population were found to be 53.3%, 38.1%, and 8.6% for the Morkaraman and 50.8%, 40.0%, and 9.2% for the Tushin, respectively. In the Hardy-Weinberg genetic equilibrium test, it was observed that the distribution of genotype frequencies was in balance (P>0.05) in the population. It has been defined that the genotype and allele frequencies determined in terms of DGAT1 gene polymorphism may be found to be sufficient to reveal the genotype diversity of the breeds. The genotype and allele frequencies determined in terms of DGAT1 gene polymorphism were sufficient to reveal the genotype diversity of the breed, the sheep with CC genotype are economically advantageous in the herd, and therefore DGAT1 gene can be used for marker-assisted selection (MAS).

Keywords

Polymorphism, DGAT1 gene, PCR-RFLP method, Sheep

Ethical Statement

Kıl örneklerinden çalışma olduğu için Fakülte Etik Kurulundan belgelendirilmiştir.

Supporting Institution

Atatürk Üniversitesi BİLİMSEL ARAŞTIRMA PROJELERİ KOORDİNASYON BİRİMİ

Project Number

FYL-2021-9187

Thanks

As the authors, we would like to thank the Atatürk University Scientific Research Projects Coordination Unit (Project number: FYL-2021-9187) for supporting the study.

References

• Abousoliman I, Reyer H, Oster M, Murani E, Mourad M, Rashed MA, Mohamed I, Wimmers K. 2020. Analysis of candidate genes for growth and milk performance traits in the Egyptian Barki sheep. Animals, 10(197): 1-13.
• Ala Noshahr F, Rafat A. 2014. Polymorphism of DGAT1 gene and its relationship with carcass weight and dressing percentage in Moghani sheep breed. Iran J Appl Anim Sci, 4(2): 331-334.
• Altwaty NH, Salem LM, Mahrous KF. 2020. Single nucleotide polymorphisms in the growth hormone receptor gene and Alu1 polymorphisms in the diacylglycerol acyltransferase 1 gene as related to meat production in sheep. Vet World, 13(5): 884.
• Anonymous. 2009. Turkey Domestic Animal Genetic Resources. URL: https://www.tarimorman.gov.tr/ TAGEM/Belgeler/ yayin/Katalog%20Türkçe.pdf (accessed date: 05.05.2021).
• Bal O, Akyüz B. 2014. Detection of Diacylglycerol O-Acyltransferase 1 (DGAT1) gene polymorphism by PCR-RFLP Method in East Anatolian Red and Native Black Cattle breeds at villages. Erciyes Üniv Vet Fak Derg, 11(1): 7-13.
• Bayraktar M, Shoshin O. 2021. Estimation of the associations between GH and DGAT1 genes and growth traits by using a decision tree in Awassi sheep. Anim Biotechnol, 2021: 1-7.
• Bayram D, Akyüz B, Arslan K, Özdemir F, Aksel EG, Çınar MU. 2019. DGAT1, CAST and IGF-I gene polymorphisms in Akkaraman lambs and their effects on live weights up to weaning age. Kafkas Univ Vet Fak Derg, 25(1): 9-15.
• Buhman KK, Smith SJ, Stone SJ, Repa JJ, Wong JS, Knapp FF, Farese RV. 2002. DGAT1 is not essential for intestinal triacylglycerol absorption or chylomicron synthesis. J Biol Chem, 277(28): 25474-25479.
• Cases S, Smith SJ, Zheng YW, Myers HM, Lear SR, Sande E, Farese RV. 1998. Identification of a gene encoding an acyl CoA: diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis. Proc Natl Acad Sci, 95(22): 13018-13023.
• Curi RA, Chardulo LAL, Arrigoni MDB, Silveira AC, de Oliveira HN. 2011. Associations between LEP, DGAT1 and FABP4 gene polymorphisms and carcass and meat traits in Nelore and crossbred beef cattle. Livest Sci, 135(2-3): 244-250.
• Dekkers JC. 2004. Commercial application of marker-and gene-assisted selection in livestock: strategies and lessons. J Anim Sci, 82(suppl_13), E313-E328.
• Esenbuğa N, Dayıoğlu H, Karaoğlu M, Kopuzlu S, Macit M. 1998. Evaluation of the use of Morkaraman and Ives lambs in early breeding in terms of herd efficiency (Fertility characteristics per 2. Fecundity sheep). Eastern Anatolia Agriculture Congress (Proceedings Book), 14-18 September 1998. Erzurum, Türkiye, 894-903.
• Geliyi C, İlaslan M. 1978. Fertility, milk and wool yields of Morkaramans raised in Kars province Karacaören village. Kars Trial and Production Station Directorate Publication Kars, Türkiye, Number: 4: 20-40.
• Gürer B, Ören MN. 2013. Food securıty analysıs for anımal products in Turkey. Cukurova Univ J Fac Eng, 29(3): 124-133.
• Köprücü E. 1975. Parameter estimates of factors affecting fertility in Merino and Morkaraman herds of Atatürk University. Ph.D Thesis, Atatürk University, Institute of Science, Department of Animal Science, Erzurum, Türkiye, pp: 20-85.
• Kumar R, Gupta S, Meena AS, Naqvi SMKI, Kumar S. 2016. Polymorphism in exon 17 of Diacylglycerol Acyltransferase-1 (DGAT-1) gene in Indian Sheep Breeds. Indian J Small Rumin, 22(2): 170-173.
• Macit M, Aksoy A. 1996. The comparison of some important production traits of Awassi and Red Karaman ewes reared in the semi-intensive conditions at Atatürk University Agricultural Research and Application Farm. Turkish J Vet Anim Sci, 20: 465-470.
• Mahrous KF, Hassanane MS, Mordy MA, Shafey Hİ, Rushdi HE. 2015. Polymorphism of some genes associated with meat‐related traits in Egyptian sheep breeds. Iran J Appl Anim Sci, 5(3): 655-663.
• Meena AS, Bhatt RS, Sahoo A, Kumar S. 2016. Genetic polymorphism of the diacylglycerol acyltransferase 1 (DGAT1) gene in Malpura sheep. Indian J Small Rumin, 1: 97-99.
• Mohammadi H, Shahrebabak MM, Sadeghi M. 2013. Association between single nucleotide polymorphism in the ovine DGAT1 gene and carcass traits in two Iranian sheep breeds. Anim Biotechnol, 24(3): 159-167.
• Nanekarani S, Kolivand M, Goodarzi M. 2016. Polymorphism of a mutation of DGAT1 gene in Lori sheep breed. J Adv Agri Tech, 3(1): 38-41.
• Noshahr FA, Rafat A. 2014. Polymorphism of DGAT1 gene and its relationship with carcass weight and dressing percentage in Moghani sheep breed. Iran J Appl Anim Sci, 4(2): 331-334.
• Öner Y, Pullu M, Akın O, Elmacı C. 2011. Investigation of Beta-lactoglobulin (β-LG) and Bovine Growth Hormone (bGH) gene polymorphisms by using HaeIII and MspI restriction enzymes in Brown Swiss and Holstein breeds reared in Bursa Region. Kafkas Univ Vet Fak Derg, 17(3): 371-376.
• Özmen O, Kul S. 2016. Identification of novel SNPs and 10 bp deletion of ovine DGAT1 gene and their association with milk production traits. Thai J Vet Med, 46(2): 271-278.
• Ranjbari M, Hashemi A, Mardani K, Darvishzadeh R. 2012. Allelic polymorphism of Makoei sheep calpastatin gene identified by a polymerase chain reaction and single-strand conformation polymorphism. J Agric Sci Technol, 14(3): 533-538.
• Reis C, Navas D, Pereira M, Cravador A. 2001. Growth hormone also polymorphism analysis in eight Portuguese bovine breeds. Archivos de Zootecnia, 50(190): 41-48.
• Sadeghi M, Mokhber M, Moradi-Shahrbabak M, Soltani V, Behroozlak M. 2020. Study of DGAT1 gene polymorphisms with carcass traits in Iranian Zel and Lori-Bakhtiari sheep breeds. Iran J Appl Anim Sci, 10(3): 501-507.
• Safari E, Fogarty NM, Gilmour AR 2005. A review of genetic parameter estimates for wool, growth, meat and reproduction traits in sheep. Livest Prod Sci, 92(3): 271-289.
• Sajad F, Reza Y, Shahram N. 2014. Study of DGAT-1 Gene Polymorphism In Mehraban-sheep by PCR-RFLP technique. New Cell Molec Biotechnol J, 4: 29-35.
• Sönmez R, Kaymakçı M, Eliçin A, Tuncel E, Wassmuth R, Taşkın, T. 2009. Improvement studies in Turkey sheep husbandry. J Agri Fac Uludag Univ, 23(2): 43-65.
• Tăbăran A, Mihaiu M, Dan SD, Reget O, Pivariu B, Cordis I, Muresan C. 2014. Identification of polymorphism in goat and sheep DGAT1 gene associated with milk production traits. Bull Univ Agric Sci Vet Med, 71(2): 281-286.
• Tambasco DD, Paz CCP, Pereira AP, Alencar MM, Freitas AR, Coutinho LL, Packer IU, Regitano LCA. 2003. Candidate genes for growth traits in beef cattle cross. J Anim Breed Genet, 120: 51-56.
• Turhan, Ş, Erdal B, Çetin B. 2010. The Formatıon of red meat and influential factors in Turkey. Turkey IX. Agricultural Economics Congress, Şanlıurfa, Türkiye, September 22-24, pp: 22-24.
• Ulusan H, Aksoy A. 1996. The performances of the yield of Tushin and Morkaraman ewes at The Farm of Veterinary Faculty University of Kafkas, Kars 2. growth and body measurements. Kafkas Univ Vet Fak Derg, 2(2): 139-146.
• Winter, A., Krämer, W., Werner, F. A., Kollers, S., Kata, S., Durstewitz, G. And Fries, R., 2002. Association of a lysine-232/alanine polymorphism in a bovine gene encoding acyl-CoA: diacylglycerol acyltransferase (DGAT1) with variation at a quantitative trait locus for milk fat content. Proceedings of the National Academy of Sciences, 99(14), 9300-9305.
• Xu QL, Chen YL, Ma RX, Xue P. 2008. Polymorphism of DGAT1 associated with intramuscular fat‐mediated tenderness in sheep. J Sci Food Agric, 89(2): 232-237.
• Yang JT, Zang RX, Liu W J, Xu HW, Bai JL, Lu JX, Wu JP. 2011. Polymorphism of a mutation of DGAT1 gene in four Chinese indigenous sheep breeds. Asian J Anim Vet Adv, 6(5): 460-468.
• Yılmaz İ, Yılmaz E. 2012. Animal food consumption and problems in Turkey. 10. National Agricultural Economics Congress, 5-7 September, Konya, Türkiye.