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Evaluation of Proximate, Phytochemicals, Antioxidant Capacity, Enzymatic Inhibition, and Anti-Inflammatory Properties of Avocado Seed Meal as Potential Feed Additive

Year 2024, Volume: 7 Issue: 5, 524 - 533, 15.09.2024
https://doi.org/10.47115/bsagriculture.1495158

Abstract

This study examined the proximate composition, phytochemical profile, antioxidant capacity, enzymatic inhibition, and anti-inflammatory properties of avocado seed meal. The proximate analysis revealed that avocado seed meal is rich in protein (17.32%), fat (15.33%), and carbohydrates (48.73%), with moderate levels of moisture (10.26%), ash (2.39%), and crude fiber (5.97%). Phytochemical analysis indicated high contents of phenols (111.56 mg/g), saponins (96.94 mg/g) and flavonoids (66.66 mg/g), but lower levels of alkaloids (14.60 mg/g), tannins (1.17 mg/g), and steroids (0.89 mg/g). The antioxidant properties assessed showed significant DPPH (50.05%) and FRAP (63.04%) free radical scavenging activities, though lower inhibition of lipid peroxidation (34.88%) and vitamin C content (0.03 mg/g). Enzymatic inhibition assays demonstrated alpha-lipase, alpha-glucosidase, and alpha-amylase inhibition rates of 31.46%, 27.56%, and 50.88%, respectively. Anti-inflammatory properties were also notable, with 24.02% albumin denaturation inhibition and 37.99% antiprotease activity. These findings suggest that avocado seed meal holds considerable potential for nutritional and therapeutic applications as natural feed supplement for livestock and humans.

Ethical Statement

Not applicable

Supporting Institution

Adekunle Ajasin University Akungba Akoko

Project Number

Nill

Thanks

I appreciate

References

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Year 2024, Volume: 7 Issue: 5, 524 - 533, 15.09.2024
https://doi.org/10.47115/bsagriculture.1495158

Abstract

Project Number

Nill

References

  • Achilonu M, Shale K, Arthur G, Naidoo K, Mbatha M. 2018. Phytochemical benefits of agroresidues as alternative nutritive dietary resource for pig and poultry farming. J Chem, 2018: 1035071. https://doi.org/10.1155/2018/1035071
  • Adeniyi SA, Orjiekwe CL, Ehiagbonare JE. 2009. Determination of alkaloids and oxalates in some selected food samples in Nigeria. Afr J Biotechnol, 8(1): 110-112.
  • Akure CO, Sekoini AB, Abeke FO, Vantsawa PA, Babasanya B, Olukotun O, Ayodele JT. 2021. Growth performance and nutrient digestibility of broiler finishers fed fermented Mucuna pruriens seed meal. Nigerian J Anim Prod, 48(3): 86-92.
  • Ambigaipalan P, de Camargo AC, Shahidi F. 2017. Identification of phenolic antioxidants and bioactives of pomegranate seeds following juice extraction using HPLC-DAD-ESI-MSn. Food Chem, 221: 1883-1894. https://doi.org/10.1016/j.foodchem.2016.10.058
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  • Ayala A, Muñoz MF, Argüelles S. 2014. Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev, 2014: 360438. https://doi.org/10.1155/2014/360438
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  • Bao J, Cai Y, Sun M, Wang G, Corke H. 2005. Anthocyanins, flavonols, and free radical scavenging activity of Chinese bayberry (Myrica rubra) extracts and their color properties and stability. J Agri Food Chem, 53(6): 2327-2332. https://doi.org/10.1021/jf048312z
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  • Dej-adisai S, Pitakbut T. 2015 'Determination of α-glucosidase inhibitory activity from selected Fabaceae plants. Pak J Pharm Sci, 28(5): 1679-1683.
  • Falowo AB, Fayemi PO, Muchenje V. 2014. Natural antioxidants against lipid–protein oxidative deterioration in meat and meat products: A review. Food Res Int, 64: 171-181.
  • Falowo AB, Mukumbo FE, Idamokoro EM, Afolayan AJ, Muchenje V. 2019. Phytochemical constituents and antioxidant activity of sweet basil (Ocimum basilicum L.) essential oil on ground beef from Boran and Nguni Cattle. Int J Food Sci, 2019: 2628747. https://doi.org/10.1155/2019/2628747.
  • Falowo AB. 2022. Potential of medicinal plants as hypocholesterolemic agents in chicken meat production. Sci Lett, 10(1): 24-31.
  • Falowo AB. 2023. Growth and immune responses, oxidative stress biomarkers, and antioxidative enzymes of broilers fed with supplementation of Chenopodium ambrosioides L. and Crassocephalum crepidioides leaf meals. Livest Stud, 63(2): 92-99. https://doi.org/10.46897/livestockstudies.1418212
  • Famuwagun AA, Taiwo KA. 2023. Drying characteristics, nutritional and anti-nutritional properties of locust bean seed. Cogent Food Agric, 9(1): 2202276, https://doi.org/10.1080/23311932.2023.2202276
  • FAO. 2013. Utilization of fruit and vegetable wastes as livestock feed and as substrates for generation of other value-added products. URL= https://fao.org (accessed date: February 23, 2024).
  • Fathi P, Moosavi-Nasab M, Mirzapour-Kouhdasht A, Khalesi M. 2021. Generation of hydrolysates from rice bran proteins using a combined ultrasonication-Alcalase hydrolysis treatment. Food Biosci, 42: 101110. https://doi.org/10.1016/j.fbio.2021.101110
  • Gyamfi MA, Yonamine M, Aniya Y. 1999. Free radical scavenging action of medicinal herbs from Ghana: Thonningia sanguinea on experimentally induced liver injuries. Gen Pharmacol, 32: 661-667. https://doi.org/10.1016/s0306-3623(98)00238-9
  • Hao XY, Yu SC, Mu CT, Wu XD, Zhang CX, Zhao JX, Zhang JX. 2020. Replacing soybean meal with flax seed meal: effects on nutrient digestibility, rumen microbial protein synthesis and growth performance in sheep. Animal, 14(9): 1841-1848.
  • Haruna ZA, Orukotan AA, Maiangwa J. 2021. Impact of fermented fruits seeds as nutrient additives on the performance of broiler birds. Sci World J, 16(3): 333-340.
  • He J, Wu ZY, Zhang S, Zhou Y, Zhao F, Peng ZQ, Hu ZW. 2014. Optimisation of microwave-assisted extraction of tea saponin and its application on cleaning of historic silks. J Surfactants Deterg, 17(5): 919-928.
  • Hotea I, Dragomirescu M, Berbecea A, Radulov I. 2023. Phytochemicals as alternatives to antibiotics in animal production. IntechOpen. https://doi.org/10.5772/intechopen.106978
  • Ikusika OO, Akinmoladun OF, Mpendulo CT. 2024. Enhancement of the nutritional composition and antioxidant activities of fruit pomaces and agro-industrial byproducts through solid-state fermentation for livestock nutrition: A review. Fermentation, 10: 227. https://doi.org/10.3390/fermentation10050227.
  • Iqbal MS, Iqbal Z, Ansari MI. 2019. A comparative anti-inflammatory and antioxidative potent of Nigella sativa seeds extract and its oil. Int J Green Pharm, 13(4): 364. https://doi.org/10.22377/ijgp.v13i04.2709
  • Kolu P, Olumide MD, Akintunde AO. 2021. Potentials of ripe Carica papaya seed meal using different processing methods as alternative feed ingredients in monogastric animal nutrition. Niger. J Anim Sci, 23(3): 177-184.
  • Kupnik K, Primožič M, Kokol V, Knez Ž, Leitgeb M. 2023. Enzymatic, antioxidant, and antimicrobial activities of bioactive compounds from avocado (Persea americana L.) seeds. Plants, 12(5): 1201. https://doi.org/10.3390/plants12051201
  • Kurek J. 2019. Introductory chapter: Alkaloids - Their importance in nature and for human life', IntechOpen. https://doi.org/10.5772/intechopen.85400.
  • Mahawan MA, Tenorio MFN, Gomez JA, Bronce AR. 2015. Characterization of flour from avocado seed kernel. Asia Pac J Multidiscip Res 3: 4.
  • Makker AOS, Goodchild AV. 1996. Quantification of tannin. A laboratory manual. International Centre of Agricultural Research in Dry Areas (ICARDA), Aleppo, Syria, pp: 25.
  • Manuelian CL, Pitino R, Simoni M, Mavrommatis A, De Marchi M, Righi F, Tsiplakou E. 2021. Plant feed additives as natural alternatives to the use of synthetic antioxidant vitamins on livestock mammals' performances, health, and oxidative status: A review of the literature in the last 20 years. Antioxidants, 10(9): 1461. https://doi.org/10.3390/antiox10091461
  • Mustafa A, Indiran MA, Shanmugham R, Ramalingam K. 2023. Anti-inflammatory activity of lauric acid, thiocolchicoside and thiocolchicoside-lauric acid formulation. Bioinformation, 19(11): 1075-1080. https://doi.org/10.6026/973206300191075.
  • Navarro DMDL, Abelilla JJ, Stein HH. 2019. Structures and characteristics of carbohydrates in diets fed to pigs: a review. J Anim Sci Biotechnol, 10: 39. https://doi.org/10.1186/s40104-019-0345-5.
  • Nyakang CO, Marete E, Ebere R, Arimi JM. 2023. Physicochemical properties of avocado seed extract model beverages and baked products incorporated with avocado seed powder. Int J Food Sci, 2023: 6860806. https://doi.org/10.1155/2023/6860806
  • Odeyemi O, Onochie AU, Odeyemi SO, Oshim IO. 2023. Evaluation of qualitative and quantitative phytochemical constituents of Napoleona imperialis stem bark. GSC Biol Pharm Sci, 24(1): 202-208.
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There are 68 citations in total.

Details

Primary Language English
Subjects Zootechny (Other)
Journal Section Research Articles
Authors

Oluwaseyi Israel Atiba 0009-0001-2367-5364

Andrew Bamidele Falowo 0000-0002-4712-0398

Olugbenga David Oloruntola 0000-0002-2175-1490

Olumuyiwa Joseph Olarotimi 0000-0002-5934-2529

Micheal Ayankoso 0000-0002-5552-144X

Project Number Nill
Publication Date September 15, 2024
Submission Date June 4, 2024
Acceptance Date August 26, 2024
Published in Issue Year 2024 Volume: 7 Issue: 5

Cite

APA Atiba, O. I., Falowo, A. B., Oloruntola, O. D., Olarotimi, O. J., et al. (2024). Evaluation of Proximate, Phytochemicals, Antioxidant Capacity, Enzymatic Inhibition, and Anti-Inflammatory Properties of Avocado Seed Meal as Potential Feed Additive. Black Sea Journal of Agriculture, 7(5), 524-533. https://doi.org/10.47115/bsagriculture.1495158

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