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Maize Seedling Responses to Combined Salinity and Temperature Stress during Early Growth Stage

Year 2025, Volume: 8 Issue: 6, 816 - 821, 15.11.2025
https://doi.org/10.47115/bsagriculture.1712136

Abstract

Maize is one of the most widely cultivated warm-season cereal crops worldwide. Its widespread use in both human and animal nutrition increases its economic importance. Salinity and temperature stresses are major abiotic factors adversely affecting maize growth and productivity. Low and high temperature extremes, along with salinity, limit plant growth and development. The aim of this study was to evaluate the response of two different maize cultivars (Zea mays indentata – Hiva and Zea mays indurata –DKC 6727) to different salinity concentrations (0, 5, 10 and 15 EC) at low (15 °C) and high temperature (30 °C). Germination percentage (GP, %), ion leakage (IL, %), shoot (SL, cm) and root length (RL, cm), and fresh (FBW, mg) and dry biomass weight (DBW, mg) data were recorded. As a result of the study, both low temperature and increased salinity levels had negative effects on all seedling-related traits in both maize varieties. DKC 6727 variety was the least affected by low temperature and high salinity levels. This research offers preliminary insights that may contribute to the screening of salt tolerance during the early growth stage and suggests that temperature could play a role in modulating the effects of salinity stress.

Ethical Statement

Ethics committee approval was not required for this study because there was no study on animals or humans.

References

  • Adhikari B, Olorunwa OJ, Barickman TC. 2022. Seed priming enhances seed germination and morphological traits of Lactuca sativa L. under salt stress. Seeds, 1: 74-86. https://doi.org/10.3390/ seeds1020007
  • Adil M, Bashir S, Bashir S, Aslam Z, Ahmad N, Younas T, Elshikh MS. 2022. Zinc oxide nanoparticles improved chlorophyll contents, physical parameters, and wheat yield under salt stress. Front Plant Sci, 13: 932861.
  • Akay H, Öztürk E, Sezer İ, Bahadır MC. 2019. Farklı tuz konsantrasyonlarının şeker mısır (Zea mays L. Var. sacharata sturt.) çeşitlerinde çimlenme ve erken fide gelişimi üzerine etkileri. TURJAF, 7: 103-108.
  • Bai T, Zhang P, Guo Z, Chetwynd AJ, Zhang M, Adeel M, Rui Y. 2021. Different physiological responses of C3 and C4 plants to nanomaterials. Envi Sci Pol Res, 28: 25542-25551.
  • Bilgili D, Atak M, Mavi K. 2018. Bazı ekmeklik buğday genotiplerinde tuz ve kuraklik stresinin çimlenme ve fide gelişimine etkisi. Mustafa Kemal Üniv Zir Fak Derg, 23(1): 85-96.
  • Çakmakçı S, Dallar A. 2019. Farklı sicaklik ve tuz konsantrasyonlarinin bazi silajlik mısır çeşitlerinin çimlenme özellikleri üzerine etkileri. Tekirdağ Üniv Derg, 16(2): 121-132.
  • Cesur A, Tabur S. 2011. Chromotoxic effects of exogenous hydrogen peroxide (H2O2) in barley seeds exposed to salt stress. Acta Physiol Plant, 33: 705-709.
  • Chen X, Zhang R, Xing Y, Jiang B, Li B, Xu X. 2021. The efficacy of different seed priming agents for promoting sorghum germination under salt stress. Plos One, 16(1): e0245505. https://doi.org/10.1371/journal. pone.0245505
  • Dwyer LM, Hayhoe HN, Culley JLB. 1990. Prediction of soil temperature from air temperature for estimating corn emergence. Can J Plant Sci, 70:619-628
  • Edalat M, Kazemeini SA. 2014. Estimation of cardinal temperatures for seedling emergence in corn. Aust J Crop Sci, 8:1072-1078.
  • Farooq M, Aziz T, Basra S, Cheema M, Rehman H. 2008. Chilling tolerance in hybrid maize induced by seed priming with salicylic acid. J Agron Crop Sc, 194, 161-168.
  • Field CB, Barros V, Stocker TF, Dahe Q, Dokken DJ, Ebi KL, Midgley PM. 2018. IPCC, 2012: summary for policymakers: managing the risks of extreme events and disasters to advance climate change adaptation. In: Planning for climate change, Routledge, pp: 111-128.
  • Gao ZF, Yang X, Mei Y, Zhang J, Chao Q, Wang BC. 2023. A dynamic phosphoproteomic analysis provides insight into the C4 plant maize (Zea mays L.) response to natural diurnal changes. Plant Jour, 113(2): 291-307.
  • Guden B, Kiemde O, Akşahin MÇ, Uzun B. 2024. Effects of salt stress on germination, seedling growth, and ion content of sweet sorghum. Mediterranean Agri Sci, 37(2): 91-98.
  • Hayhoe HN, Dwyer LM. 1990. Relationship between percentage emergence and growing degree days for corn can. J Soil Sci, 70: 493-497. doi: 10.4141/cjss90-048
  • Hellal FA, El-Shabrawi HM, Abd El-Hady M, Khatab IA, El-Sayed SAA, Abdellly C. 2018. Influence of PEG induced drought stress on molecular and biochemical constituents and seedling growth of Egyptian barley cultivars. J Genet Eng Biotechnol, 16(1): 203-212.
  • Idikut L, Dumlupinar Z, Kara SN, Yururdurmaz C, Çolkese M. 2012. The effect of different temperatures and salt concentrations on some popcorn landraces and hybrid corn genotype germinations. Pak J Bot, 44(2): 579-587.
  • Islam M, Islam M, Hasan M, Hafeez A, Chowdhury M, Pramanik M, El Sabagh A. 2024. Salinity stress in maize: consequences, tolerance mechanisms, and management strategies. OBM Genetics, 8(2). https://doi.org/10.21926/obm.genet.2402232
  • Khaeim H, Kende Z, Jolánkai M, Kovács GP, Gyuricza C, Tarnawa Á. 2022. Impact of temperature and water on seed germination and seedling growth of maize (Zea mays L.). Agronomy, 12: 397. https://doi.org/10.3390/ agronomy12020397
  • Khalid N, Tarnawa Á, Balla I, Omar S, Abd Ghani R, Jolánkai M, Kende, Z. 2023. Combination effect of temperature and salinity stress on germination of different maize (Zea mays L.) varieties. Agric, 2023, 13: 1932. https://doi.org/10.3390/agriculture13101932
  • Munns R, Tester M. 2008. Mechanisms of salinity tolerance. Annua Rev Plant Biol, 59(1): 651-681. https://doi.org/10.1146/annurev.arplant.59.032607.092911
  • Okumuş O, Kahraman ND, Oğuz MÇ, Yıldız M. 2023. Magnetic field treatment in barley: improved salt tolerance in early stages of development. Selcuk J Agric Food Sci, 37(3): 556-569.
  • Okumuş O, Say A, Eren B, Demirel F, Uzun S, Yaman M, Aydın A. 2024a. Using machine learning algorithms to investigate the impact of temperature treatment and salt stress on four forage peas (Pisum sativum var. arvense L.). Horticulturae, 10(6): 656.
  • Okumuş O, Şekerci AD, Uzun S. 2024b. The single and interactive effect of salinity and temperature on germination characteristics of italian ryegrass (Lolium multiflorum Lam.) Seeds. BSJ Agri, 7(5): 563-569. https://doi.org/10.47115/bsagriculture.1525082
  • Okumuş O, Şekerci AD. 2024c. Effects of different salt stress and temperature applications on germination in mung bean (Vigna radiata (L.) R. Wilczek) genotypes. BS Agri, 7(3): 310-316. https://doi.org/10.47115/bsagriculture.1457295
  • Omid A, Farzad SZ. 2012. Osmo and hydro priming improvement germination characteristics and enzyme activity of Mountain rye (Secale montanum) seeds under drought stress. J Stress Physiol Biochem, 8: 253-261.
  • Öner M. 2023. Mısır (Zea mays. L.) bitkisinin çimlenme ve fide dönemlerinde uygulanan kitosanın fizyolojik ve morfolojik özellikler üzerine etkisi. Sakarya Uygulamalı Bilimler Üniversitesi Lisansüstü Eğitim Enstitüsü, Sakarya, Türkiye, pp: 78.
  • Pangapanga-Phiri I, Mungatana ED. 2021. Adoption of climate-smart agricultural practices and their influence on the technical efficiency of maize production under extreme weather events. Int J Disaster Risk Reduct, 61: 102322.
  • Rajjou L, Duval M, Gallardo K, Catusse J, Bally J, Job C. 2012. Seed germination and vigor. Annua Rev Plant Biol, 63(1): 507-533. https://doi.org/10.1146/annurev-arplant-042811- 105550
  • Sarwar N, Atique-ur-Rehman Farooq O, Wasaya A, Hussain M, El-Shehawi AM, Ahmad S, Brestic M, Mahmoud SF, Zivcak M. 2021. Integrated nitrogen management improves productivity and economic returns of wheat-maize cropping system. J King Saud Univ Sci, 33: 101475.
  • Schauberger B, Archontoulis S, Arneth A, Balkovic J, Ciais P, Deryng D, Elliott J, Folberth C, Khabarov N, Müller C. 2017. Consistent negative response of US crops to high temperatures in observations and crop models. Nat Commun, 8:13931. https://doi.org/10.1038/ncomms13931
  • Tlahig S, Bellani L, Karmous I, Barbieri F, Loumerem M, Muccifora S. 2021. Response to salinity in legume species: An insight on the effects of salt stress during seed germination and seedling growth. Chem Biodiver, 18(4): e2000917.
  • Wang T, Zang Z, Wang S, Liu Y, Wang H, Wang W, Hu X, Sun J, Tai F, He R. 2021. Quaternary ammonium iminofullerenes promote root growth and osmotic-stress tolerance in maize via ROS neutralization and improved energy status. Plant Physiol Biochem, 164: 122-131.
  • Waqas MA, Wang X, Zafar SA, Noor MA, Hussain HA, Azher Nawaz M, Farooq M. 2021. Thermal stresses in maize: effects and management strategies. Plants, 10(2): 293.
  • Xu C, Li R, Song W, Wu T, Sun S, Han T, Wu C. 2021. High density and uniform plant distribution improve soybean yield by regulating population uniformity and canopy light interception. Agronomy, 11(9): 1880.
  • Xue X, Du S, Jiao F, Xi M, Wang A, Xu H, Jiao Q, Zhang X, Jiang H, Chen J. 2021. The regulatory network behind maize seed germination: Effects of temperature, water, phytohormones, and nutrients. Crop J, 9: 901-914.
  • Zhang H, Zhang J, Xu Q, Wang D, Di H, Huang J, Zhou Y. 2020. Identification of candidate tolerance genes to low-temperature during maize germination by GWAS and RNA-seq approaches. BMC Plant Bio, 20: 1-17.
  • Zheng C, Jiang D, Liu F, Li X, Liu W, Jing Q. 2009. Exogenous nitric oxide improves seed germination in wheat against mitochondrial oxidative damage induced by high salinity. Environ Exp Bot, 67(1): 222-227.
  • Zhu G, An L, Jiao X, Chen X, Zhou G, McLaughlin N. 2019. Effects of gibberellic acid on water uptake and germination of sweet sorghum seeds under salinity stress. Chil J Agric Res, 79(3): 415-424.

Maize Seedling Responses to Combined Salinity and Temperature Stress during Early Growth Stage

Year 2025, Volume: 8 Issue: 6, 816 - 821, 15.11.2025
https://doi.org/10.47115/bsagriculture.1712136

Abstract

Maize is one of the most widely cultivated warm-season cereal crops worldwide. Its widespread use in both human and animal nutrition increases its economic importance. Salinity and temperature stresses are major abiotic factors adversely affecting maize growth and productivity. Low and high temperature extremes, along with salinity, limit plant growth and development. The aim of this study was to evaluate the response of two different maize cultivars (Zea mays indentata – Hiva and Zea mays indurata –DKC 6727) to different salinity concentrations (0, 5, 10 and 15 EC) at low (15 °C) and high temperature (30 °C). Germination percentage (GP, %), ion leakage (IL, %), shoot (SL, cm) and root length (RL, cm), and fresh (FBW, mg) and dry biomass weight (DBW, mg) data were recorded. As a result of the study, both low temperature and increased salinity levels had negative effects on all seedling-related traits in both maize varieties. DKC 6727 variety was the least affected by low temperature and high salinity levels. This research offers preliminary insights that may contribute to the screening of salt tolerance during the early growth stage and suggests that temperature could play a role in modulating the effects of salinity stress.

Ethical Statement

Ethics committee approval was not required for this study because there was no study on animals or humans.

References

  • Adhikari B, Olorunwa OJ, Barickman TC. 2022. Seed priming enhances seed germination and morphological traits of Lactuca sativa L. under salt stress. Seeds, 1: 74-86. https://doi.org/10.3390/ seeds1020007
  • Adil M, Bashir S, Bashir S, Aslam Z, Ahmad N, Younas T, Elshikh MS. 2022. Zinc oxide nanoparticles improved chlorophyll contents, physical parameters, and wheat yield under salt stress. Front Plant Sci, 13: 932861.
  • Akay H, Öztürk E, Sezer İ, Bahadır MC. 2019. Farklı tuz konsantrasyonlarının şeker mısır (Zea mays L. Var. sacharata sturt.) çeşitlerinde çimlenme ve erken fide gelişimi üzerine etkileri. TURJAF, 7: 103-108.
  • Bai T, Zhang P, Guo Z, Chetwynd AJ, Zhang M, Adeel M, Rui Y. 2021. Different physiological responses of C3 and C4 plants to nanomaterials. Envi Sci Pol Res, 28: 25542-25551.
  • Bilgili D, Atak M, Mavi K. 2018. Bazı ekmeklik buğday genotiplerinde tuz ve kuraklik stresinin çimlenme ve fide gelişimine etkisi. Mustafa Kemal Üniv Zir Fak Derg, 23(1): 85-96.
  • Çakmakçı S, Dallar A. 2019. Farklı sicaklik ve tuz konsantrasyonlarinin bazi silajlik mısır çeşitlerinin çimlenme özellikleri üzerine etkileri. Tekirdağ Üniv Derg, 16(2): 121-132.
  • Cesur A, Tabur S. 2011. Chromotoxic effects of exogenous hydrogen peroxide (H2O2) in barley seeds exposed to salt stress. Acta Physiol Plant, 33: 705-709.
  • Chen X, Zhang R, Xing Y, Jiang B, Li B, Xu X. 2021. The efficacy of different seed priming agents for promoting sorghum germination under salt stress. Plos One, 16(1): e0245505. https://doi.org/10.1371/journal. pone.0245505
  • Dwyer LM, Hayhoe HN, Culley JLB. 1990. Prediction of soil temperature from air temperature for estimating corn emergence. Can J Plant Sci, 70:619-628
  • Edalat M, Kazemeini SA. 2014. Estimation of cardinal temperatures for seedling emergence in corn. Aust J Crop Sci, 8:1072-1078.
  • Farooq M, Aziz T, Basra S, Cheema M, Rehman H. 2008. Chilling tolerance in hybrid maize induced by seed priming with salicylic acid. J Agron Crop Sc, 194, 161-168.
  • Field CB, Barros V, Stocker TF, Dahe Q, Dokken DJ, Ebi KL, Midgley PM. 2018. IPCC, 2012: summary for policymakers: managing the risks of extreme events and disasters to advance climate change adaptation. In: Planning for climate change, Routledge, pp: 111-128.
  • Gao ZF, Yang X, Mei Y, Zhang J, Chao Q, Wang BC. 2023. A dynamic phosphoproteomic analysis provides insight into the C4 plant maize (Zea mays L.) response to natural diurnal changes. Plant Jour, 113(2): 291-307.
  • Guden B, Kiemde O, Akşahin MÇ, Uzun B. 2024. Effects of salt stress on germination, seedling growth, and ion content of sweet sorghum. Mediterranean Agri Sci, 37(2): 91-98.
  • Hayhoe HN, Dwyer LM. 1990. Relationship between percentage emergence and growing degree days for corn can. J Soil Sci, 70: 493-497. doi: 10.4141/cjss90-048
  • Hellal FA, El-Shabrawi HM, Abd El-Hady M, Khatab IA, El-Sayed SAA, Abdellly C. 2018. Influence of PEG induced drought stress on molecular and biochemical constituents and seedling growth of Egyptian barley cultivars. J Genet Eng Biotechnol, 16(1): 203-212.
  • Idikut L, Dumlupinar Z, Kara SN, Yururdurmaz C, Çolkese M. 2012. The effect of different temperatures and salt concentrations on some popcorn landraces and hybrid corn genotype germinations. Pak J Bot, 44(2): 579-587.
  • Islam M, Islam M, Hasan M, Hafeez A, Chowdhury M, Pramanik M, El Sabagh A. 2024. Salinity stress in maize: consequences, tolerance mechanisms, and management strategies. OBM Genetics, 8(2). https://doi.org/10.21926/obm.genet.2402232
  • Khaeim H, Kende Z, Jolánkai M, Kovács GP, Gyuricza C, Tarnawa Á. 2022. Impact of temperature and water on seed germination and seedling growth of maize (Zea mays L.). Agronomy, 12: 397. https://doi.org/10.3390/ agronomy12020397
  • Khalid N, Tarnawa Á, Balla I, Omar S, Abd Ghani R, Jolánkai M, Kende, Z. 2023. Combination effect of temperature and salinity stress on germination of different maize (Zea mays L.) varieties. Agric, 2023, 13: 1932. https://doi.org/10.3390/agriculture13101932
  • Munns R, Tester M. 2008. Mechanisms of salinity tolerance. Annua Rev Plant Biol, 59(1): 651-681. https://doi.org/10.1146/annurev.arplant.59.032607.092911
  • Okumuş O, Kahraman ND, Oğuz MÇ, Yıldız M. 2023. Magnetic field treatment in barley: improved salt tolerance in early stages of development. Selcuk J Agric Food Sci, 37(3): 556-569.
  • Okumuş O, Say A, Eren B, Demirel F, Uzun S, Yaman M, Aydın A. 2024a. Using machine learning algorithms to investigate the impact of temperature treatment and salt stress on four forage peas (Pisum sativum var. arvense L.). Horticulturae, 10(6): 656.
  • Okumuş O, Şekerci AD, Uzun S. 2024b. The single and interactive effect of salinity and temperature on germination characteristics of italian ryegrass (Lolium multiflorum Lam.) Seeds. BSJ Agri, 7(5): 563-569. https://doi.org/10.47115/bsagriculture.1525082
  • Okumuş O, Şekerci AD. 2024c. Effects of different salt stress and temperature applications on germination in mung bean (Vigna radiata (L.) R. Wilczek) genotypes. BS Agri, 7(3): 310-316. https://doi.org/10.47115/bsagriculture.1457295
  • Omid A, Farzad SZ. 2012. Osmo and hydro priming improvement germination characteristics and enzyme activity of Mountain rye (Secale montanum) seeds under drought stress. J Stress Physiol Biochem, 8: 253-261.
  • Öner M. 2023. Mısır (Zea mays. L.) bitkisinin çimlenme ve fide dönemlerinde uygulanan kitosanın fizyolojik ve morfolojik özellikler üzerine etkisi. Sakarya Uygulamalı Bilimler Üniversitesi Lisansüstü Eğitim Enstitüsü, Sakarya, Türkiye, pp: 78.
  • Pangapanga-Phiri I, Mungatana ED. 2021. Adoption of climate-smart agricultural practices and their influence on the technical efficiency of maize production under extreme weather events. Int J Disaster Risk Reduct, 61: 102322.
  • Rajjou L, Duval M, Gallardo K, Catusse J, Bally J, Job C. 2012. Seed germination and vigor. Annua Rev Plant Biol, 63(1): 507-533. https://doi.org/10.1146/annurev-arplant-042811- 105550
  • Sarwar N, Atique-ur-Rehman Farooq O, Wasaya A, Hussain M, El-Shehawi AM, Ahmad S, Brestic M, Mahmoud SF, Zivcak M. 2021. Integrated nitrogen management improves productivity and economic returns of wheat-maize cropping system. J King Saud Univ Sci, 33: 101475.
  • Schauberger B, Archontoulis S, Arneth A, Balkovic J, Ciais P, Deryng D, Elliott J, Folberth C, Khabarov N, Müller C. 2017. Consistent negative response of US crops to high temperatures in observations and crop models. Nat Commun, 8:13931. https://doi.org/10.1038/ncomms13931
  • Tlahig S, Bellani L, Karmous I, Barbieri F, Loumerem M, Muccifora S. 2021. Response to salinity in legume species: An insight on the effects of salt stress during seed germination and seedling growth. Chem Biodiver, 18(4): e2000917.
  • Wang T, Zang Z, Wang S, Liu Y, Wang H, Wang W, Hu X, Sun J, Tai F, He R. 2021. Quaternary ammonium iminofullerenes promote root growth and osmotic-stress tolerance in maize via ROS neutralization and improved energy status. Plant Physiol Biochem, 164: 122-131.
  • Waqas MA, Wang X, Zafar SA, Noor MA, Hussain HA, Azher Nawaz M, Farooq M. 2021. Thermal stresses in maize: effects and management strategies. Plants, 10(2): 293.
  • Xu C, Li R, Song W, Wu T, Sun S, Han T, Wu C. 2021. High density and uniform plant distribution improve soybean yield by regulating population uniformity and canopy light interception. Agronomy, 11(9): 1880.
  • Xue X, Du S, Jiao F, Xi M, Wang A, Xu H, Jiao Q, Zhang X, Jiang H, Chen J. 2021. The regulatory network behind maize seed germination: Effects of temperature, water, phytohormones, and nutrients. Crop J, 9: 901-914.
  • Zhang H, Zhang J, Xu Q, Wang D, Di H, Huang J, Zhou Y. 2020. Identification of candidate tolerance genes to low-temperature during maize germination by GWAS and RNA-seq approaches. BMC Plant Bio, 20: 1-17.
  • Zheng C, Jiang D, Liu F, Li X, Liu W, Jing Q. 2009. Exogenous nitric oxide improves seed germination in wheat against mitochondrial oxidative damage induced by high salinity. Environ Exp Bot, 67(1): 222-227.
  • Zhu G, An L, Jiao X, Chen X, Zhou G, McLaughlin N. 2019. Effects of gibberellic acid on water uptake and germination of sweet sorghum seeds under salinity stress. Chil J Agric Res, 79(3): 415-424.
There are 39 citations in total.

Details

Primary Language English
Subjects Agricultural Engineering (Other)
Journal Section Research Articles
Authors

Birgul Guden 0000-0002-7375-6533

Onur Okumuş 0000-0001-6957-3729

Early Pub Date November 14, 2025
Publication Date November 15, 2025
Submission Date June 3, 2025
Acceptance Date October 17, 2025
Published in Issue Year 2025 Volume: 8 Issue: 6

Cite

APA Guden, B., & Okumuş, O. (2025). Maize Seedling Responses to Combined Salinity and Temperature Stress during Early Growth Stage. Black Sea Journal of Agriculture, 8(6), 816-821. https://doi.org/10.47115/bsagriculture.1712136

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