In Vitro Fertilization of Immature Bovine Oocytes Matured in a Protein-Free Media and Their Subsequent Development to the Blastocysts

Abstract

Fetal calf serum obtained from unborn calves causes pain to the calf. As a result of the ongoing discussions on issues such as less suffering to animals and animal welfare, especially in recent years, there has been a lot of research on the possibilities of using fetal serum substitutes in in vitro embryo and cell culture. However, the use of serum may cause some conflicts, especially in studies investigating single molecular pathways, due to the effects of molecules present in serum and whose concentration cannot be determined precisely on cellular functions. For this purpose, in this study, in which the possibilities of using serum substitutes were investigated, it was determined that the use of knockout serumTM for in vitro maturation significantly increased the number of embryos developing until the first division and blastocyst. On the other hand, it was determined that the use of synthetic serum substitution and serum substitution 2 slightly reduced the number of embryos obtained.

Keywords

• IVF
• Serum replacement
• Bovine
• Maturation
• Blastocyst
• Oocyte

Protein İçermeyen Medya İle Olgunlaştırılan İmmatür Sığır Oositlerinin İn Vitro Fertilizasyonu ve Blastosiste Kadar Gelişimleri

Abstract

Henüz doğmamış buzağılardan elde edilen fötal buzağı serumu, buzağının acı çekmesine neden olmaktadır. Hayvanlara daha az acı çektirilmesi ve hayvan refahı gibi konularda süregelen tartışmalar neticesinde özellikle son yıllarda, in vitro embriyo ve hücre kültüründe fötal serum ikamelerinin kullanılma olanaklarına dair çok sayıda araştırma yapılmaktadır. Bununla birlikte serum içerisinde mevcut olan ve konsatrasyonu tam olarak belirlenemeyen moleküllerin hücresel işlevler üzerine etkilerinden dolayı özellikle tekli moleküler yolakların araştırıldığı çalışmalarda serum kullanımı bazı çakışmalara neden olabilmektedir. Bu amaçla, serum ikamelerinin kullanım olanaklarının araştırıldığı bu çalışmada, in vitro matürasyon için knockout serumTM kullanımının ilk bölünme ve blastosiste kadar gelişen embriyo sayısının önemli oranda artırdığı tespit edilmiştir. Bununla birlikte sentetik serum ikamesi ve serum ikamesi 2 kullanımının ise elde edilen embriyo sayılarını bir miktar düşürdüğü belirlenmiştir.

Keywords

• IVF
• Serum ikamesi
• Sığır
• Olgunlaştırma
• Blastosist
• Oosit

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