A New Spectrophotometric Method for Determination of Carvedilol from Tablet

Abstract

In this study, a new spectrophotometric method was developed for the quantitative analysis of Carvedilol and the method was validated. The method depends on the reaction between the carvedilol and 1,2,5,8-tetrahydroxyanthraquinone in methanol to yield colored charge transfer complex giving maximum absorbance at 560 nm. For optimization of the proposed method, several parameters were investigated such as solvent type, reaction time, and quinalizarin concentration. The stoichiometry of colored charge transfer complex was found to be 2:1(reagent: drug) by Job’s method. Beer’s Law is obeyed in the concentration range of 0.5-60 µg/mL with 0.9986 correlation efficient. Limit of detection (LOD) and limit of quantifiqation (LOQ) were found 0.147 µg/mL, 0.491 µg/mL, respectively. The proposed method can be successfully applied pharmaceutical formulation.

Keywords

• Charge transfer complex
• Job’s method
• Molecular absorption spectroscopy

References

1. Altunay N, Haq HU, Castro-Mu˜noz R. 2023. Optimization of vortex-assisted hydrophobic magnetic deep eutectic solvent-based dispersive liquid phase microextraction for quantification of niclosamide in real samples. Food Chem, 426: 136646.
2. Briann D, William TA. 2004. Pharmacology of carvedilol. Am J Cardiol, 93: 3B–6B.
3. Gouda AA, Kasssem M. 2012. Novel spectrophotometric methods for determination of desloratidine in pharmaceutical formulations based on charge transfer reaction. Arabian J Chem, 9: 1712-1720. https://doi.org/10.1016/j.arabjc.2012.04.050.
4. Junwei L, Wang L, Wang S, Chen M, Hu EG, Ge R 2015. Simultaneous quantification of carvedilol and its metabolites in rat plasma by ultra-performance liquid chromatography tandem mass spectrometry and pharmacokinetic application. J Chromatograp B, 974: 138-146. https://doi.org/10.1016/j.jchromb.2014.10.037.
5. Kelani K, Bebawy LI, Abdel-Fattah L, Ahamad AS. 1997. Spectrophotometric determination of some n-donating drugs using DDQ Anal. Letters, 10(30): 1843–1860.
6. Kirtimaya M, Kumar BK, Kumari MM, Subrahmanyam BSS. 2016. New analytical method development and validation of chlorpheniramine maleate by using UV-visible spectrophotometry. Indo American J Pharmaceut Sci, 7: 767-772.
7. Korkmaz D, Demir C, Aydın F, Ataman OY. 2005. Cold vapour generation and on-line trapping of cadmium species on quartz surface prior to detection by atomic absorption spectrometry. J Anal At Spectrom, 20: 46-52.
8. Moffat AC, Osselton MD, Widdop B. 2004. Clarke’s analysis of drugs and poisons. Pharmaceutical Press, London, UK, pp: 2736.

9. Mohamed EM, Frag YZ, Hathoot AA, Shalaby EA. 2018. Molecular and biomolecular spectroscopy. Spectrochimica Acta Part A: 189: 357-365. https://doi.org/10.1016/j.saa.2017.08.027.
10. Mutlu B. 2010. Vasodilatator Beta blockers in cardiovascular disease. Trakya Univ J Medic Fac, 27: 26-30.
11. Ragaa El-S, Ayman AG, El-Azzazy R. 2013. Spectrophotometric Study on the charge transfer complex between sumatriptan succinate and some Π-acceptors and alizarin derivatives. Chem Indust Chem Eng Quart, 4: 529-540. https://doi.org/10.2298/CICEQ1205133087E.96.
12. Rani YN, Ravi BV, Kumar V, Smitapadma M. 2013. Development and validation of new analytical methods for the estimation of carvedilol in bulk and pharmaceutical dosage. Asian J Pharmaceut Clin Res, 6: 2.
13. Soleymanpour A, Ghasemian M. 2015. Chemically modified carbon paste sensor for the potentiometric determination of carvedilol in pharmaceutical and biological media. Measurement, 59: 14-20. https://doi.org/10.1016/j.Measurement.2014.09.046.
14. Streim K., Sponer, G, MullerBeckmann, B, Bartsch, WJ. 1987. Cardiovasc. Pharmacol, 10: 33.
15. URL1: https://database.ich.org/ (accessed date: November 12, 2023).
16. Vale GT, Ceron CS, Gonzaga NA, Simplicio JA, Padovan JC. 2019. Three Generations of β-blockers: History, class differences and clinical applicability. Curr Hypertens Rev, 15: 22. https://doi.org/10.2174/1573402114666180918102735.
17. Verma JK, Syed HA. 2007. Extractive spectrophotometric method for determination of carvedilol in tablets. Indian J Pharm Sci, 2: 303-304. https://doi.org/10.4103/0250-474X.33166.