Investigation of Antibacterial Activities of Elettaria Cardamomum (L.) Maton Extracts Prepared with Different Solvents

Abstract

The aim of this study is to determine the antibacterial activities of extracts prepared from the seeds of Elettaria cardamomum against various bacteria. In this study, extracts were prepared from the seeds of the Elettaria cardamomum plant using different solvents (ethanol, acetone, ethyl acetate, chloroform, cold water, hot water). The antibacterial activities of the extracts against five pathogenic bacterial strains (Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213, Bacillus subtilis ATCC 6633, Klebsiella pneumoniae ATCC 700603, Pseudomonas aeruginosa ATCC 29853) were determined using the disc diffusion method. Study results showed that ethanol, acetone, ethyl acetate and chloroform extracts had different degrees of antibacterial activity against Staphylococcus aureus ATCC 29213, Bacillus subtilis ATCC 6633 and Klebsiella pneumoniae ATCC 700603 bacteria. All extracts in the study did not show antibacterial activity against Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 29853 bacteria. Ethyl acetate extract showed the highest antibacterial activity against Staphylococcus aureus ATCC 29213 bacteria.

Keywords

• Antibacterial activity
• Elettaria cardamomum
• Different solvents
• Extract

Farklı Çözücülerle Hazırlanan Elettaria Cardamomum (L.) Maton Ekstraktlarının Antibakteriyel Aktivitelerinin İncelenmesi

Abstract

Bu çalışmanın amacı Elettaria cardamomum’un tohumlarından hazırlanan ekstraktların çeşitli bakterilere karşı olan antibakteriyel aktivitelerinin belirlenmesidir. Bu çalışmada, Elettaria cardamomum bitkisinin tohumlarından farklı çözücüler (etanol, aseton, etil asetat, kloroform, soğuk su, sıcak su) kullanılarak ekstraktları hazırlanmıştır. Ekstraktların beş patojen bakteri suşuna (Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213, Bacillus subtilis ATCC 6633, Klebsiella pneumoniae ATCC 700603, Pseudomonas aeruginosa ATCC 29853) karşı olan antibakteriyel aktiviteleri disk difüzyon yöntemi kullanılarak belirlenmiştir. Çalışma sonuçları etanol, aseton, etil asetat ve kloroform ekstraktlarının Staphylococcus aureus ATCC 29213, Bacillus subtilis ATCC 6633 ve Klebsiella pneumoniae ATCC 700603 bakterilerine karşı farklı derecelerde antibakteriyel aktiviteye sahip olduğunu göstermiştir. Çalışmadaki tüm ekstraktlar Escherichia coli ATCC 25922 ve Pseudomonas aeruginosa ATCC 29853 bakterilerine karşı antibakteriyel aktivite göstermemiştir. En yüksek antibakteriyel aktiviteyi etil asetat ekstraktı Staphylococcus aureus ATCC 29213 bakterisine karşı göstermiştir.

Keywords

• Antibakteriyel aktivite
• Elettaria cardamomum
• Farklı çözücüler
• Ekstrakt

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