Aspir (Carthamus tinctorius L.) ile Gümüş Nanoparçacıkların Yeşil Sentezi, Karakterizasyonu ve Antimikrobiyal Aktivitesi

Abstract

Bu çalışmanın amacı, aspir (Carthamus tinctorius L.) bitkisinin yaprak, çiçek ve tohum ekstraktları kullanılarak gümüş nanoparçacıkların (AgNP) sentezlenmesi ve bu nanoparçacıkların antimikrobiyal aktivitelerinin değerlendirilmesidir. Bu kapsamda bitki ekstraktları indirgeme ve kaplama ajanı olarak kullanılmış; sentezlenen AgNP’ler UV–Vis spektroskopisi, SEM-EDX ve FTIR analizleri ile karakterize edilmiştir. Ayrıca çiçek ekstraktının kimyasal bileşimi GC-MS ile belirlenmiştir. AgNP’lerin antimikrobiyal aktivitesi disk difüzyon yöntemi kullanılarak Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Saccharomyces cerevisiae ve Candida albicans üzerinde test edilmiştir. Bulgular, en yüksek sentez veriminin ve en düzenli nanopartikül morfolojisinin çiçek ekstraktı ile elde edildiğini ve ortalama boyutu 40 nm olan AgNP’lerin oluştuğunu göstermiştir. Çiçek ekstraktıyla sentezlenen AgNP’ lerin özellikle B. subtilis, P. aeruginosa ve E. coli suşlarında en geniş inhibisyon zonlarını oluşturduğu belirlenmiştir, mantar türlerinde ise inhibisyon gözlenmemiştir. Genel olarak, aspir çiçeği kaynaklı AgNP’lerin potansiyel antimikrobiyal ajanlar olarak değerlendirilebileceği sonucuna varılmıştır.

Keywords

• Yeşil sentez
• Gümüş nanoparçacıklar (AgNP’ler)
• Carthamus tinctorius L. (Aspir)
• Antimikrobiyal aktivite
• Nanobiyoteknoloji

Green Synthesis, Characterization and Antimicrobial Activity of Silver Nanoparticles with Safflower (Carthamus tinctorius L.)

Abstract

The aim of this study was to synthesize silver nanoparticles (AgNPs) using the leaf, flower, and seed extracts of safflower (Carthamus tinctorius L.) and to evaluate their antimicrobial activities. For this purpose, plant extracts were used as reducing and stabilizing agents, and the synthesized AgNPs were characterized by UV–Vis spectroscopy, SEM-EDX, and FTIR analyses. In addition, the chemical composition of the flower extract was determined by GC-MS. The antimicrobial activity of the AgNPs was tested against Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Saccharomyces cerevisiae and Candida albicans using the disk diffusion method. The results showed that the highest synthesis efficiency and the most regular nanoparticle morphology were demonstrate from the flower extract, producing AgNPs with an average size of approximately 40 nm. It has been determined that AgNPs synthesized with flower extract form the widest inhibition zones, particularly in B. subtilis, P. aeruginosa, and E. coli strains, while no inhibition was observed in fungal species. Overall, it has been concluded that AgNPs derived from cornflowers can be considered as potential antimicrobial agents.

Keywords

• Green synthesis
• Silver nanoparticles (AgNPs)
• Carthamus tinctorius L. (Safflower)
• Antimicrobial activity
• Nanobiotechnology

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