Determination of betalactoglobulin gene polymorphism in Kivircik sheep

Abstract

This study was carried out to determine the genetic polymorphism of the β-lactoglobulin (β-Lg) gene for indigenous Kivircik breed in Turkey. As to find the Kivircik Sheep is limited as purebred, this breed was raised and maintained in special farm with small numbers as pure breeds. Due to animal material was constituted 48 heads as purebred Kivircik sheep. The study was realized by means of PCR-RFLP methods. Also, two genetic alleles (A and B) and three genotypes (AA, AB and BB) were detected for this purebred. The gene frequencies of β-Lg A and B were calculated as 0.469 and 0.531 in Kivircik sheep. The population was found in equilibrium according to Hardy-Weinberg. At the same time, Some F statistics values were calculated like effective alleles number (Ne), observed heterozygosity (Ho), expected heterozygosity (He) and fixation index (Fis); 1.992, 0.396, 0.498 and 0.205, respectively.

Keywords

• Kivircik,β-Lg,Milk protein,Polymorphism,PCR-RFLP

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