Anatomical, Ecological and Trichome Micro-Morphological Features of Two Marrubium L. Taxa (Lamiaceae)

Year 2024, Volume: 7 Issue: 1, 43 - 54, 15.01.2024

Kamer Volkan Koçak Nezahat Kandemir

https://doi.org/10.34248/bsengineering.1324086

Abstract

The anatomical, ecological and trichome micro-morphological features of two taxa (Marrubium amasiensis and Marrubium parviflorum subsp. parviflorum) belonging to the genus Marrubium were determined and these features were compared. M. amasiensis is endemic and is distributed only around Amasya in Türkiye. In anatomical examinations, cross sections were taken from the root, stem, petiole, leaves and surface sections were taken from stem, petiole, and the lower and upper surfaces of the leaves. When the anatomical findings were examined, multi-layered lamellar collenchyma layer was encountered at the corners of the stem. The studied taxa have equifacial leaves in terms of mesophyll structure. Stomata of M. amasiensis are anomocytic and rarely anisocytic type, while stomata of M. parviflorum subsp. parviflorum are anomocytic type. The margins of the adjacent cells of the stomata in both taxa are very undulate. Eglandular and glandular trichomes were seen on vegetative organs of the two taxa. The eglandular trichomes are stellate, dendroid, marrubioid and simple unicellular types, while glandular trichomes are capitate and peltate types. Dense stellate trichomes were found in the vegetative organs of the studied taxa. Since the head and stalk cell numbers of capitate trichomes showed variation, these trichomes were divided into subtypes. Three subtypes of capitate trichomes were found on the vegetative organs of M. parviflorum subsp. parviflorum, while four subtypes of capitate trichomes were found on the vegetative organs of M. amasiensis. Soil samples were taken during flowering periods of taxa, physical and chemical properties were determined. Some similarities and differences were detected in the anatomical, ecological and trichome micro-morphological features of the studied two taxa. It has been emphasized that these different features will be used as valuable taxonomic characters in distinguishing of the two taxa.

Keywords

Marrubium taxa, Anatomy, Ecology, Trichome micro-morphology, Lamiaceae, Türkiye

Supporting Institution

There is no institution supporting the study of this article.

Project Number

no project number

References

• Ahvazi M, Jamzad Z, Balali GR, Saeidi H. 2016. Trichome micro-morphology in Marrubium L. (Lamiaceae) in Iran and the role of environmental factors on their variation. Iran J Bot, 22: 39-58.
• Akbulut TD, Baykan S. 2023. Morphological and micromorphological investigation of Marrubium L. (Lamiaceae) taxons distributedin the Aegean Region. J Fac Pharm, 47(1): 51-75.
• Akgül G, Ketenoğlu O, Doğan M. 2017. A new species of Marrubium L. (Lamiaceae) from Turkey. J Herb Syst Bot, 24: 37-46.
• Anhar AY, Soad AH, Zeinab AE, Hassan MI, Omayma AE. 2018. Morphological and anatomical studies on selected Lamiaceae medicinal plants in Bani Matar district, Sana'a (Yemen). Taeckholmia, 38: 17-39.
• Benzidane N, Smahi R, Zabouche B, Makrouf A, Arrar L. 2020. Phytochemical study and antimicrobial activity of Algerian Marrubium vulgare leaf and stem extracts. J Drug Deliv Ther, 10(5):70-74.
• Büyükkartal HN, Çölgeçen H, Akgül G. 2016. Comparative leaf, stem and root anatomy of taxa Marrubium bourgaei and Marrubium heterodon (Lamiaceae). Aust J Crop Sci, 10(11): 1516-1522.
• Büyüköztürk, Ş. 2001. Data analysis for social science research design and statistical manual SPSS applications and review. Pegem Publication, Ankara, Türkiye, pp: 216.
• Cantino PD. 1990. The phylogenetic significance of stomata and trichomes in the Labiatae and Verbenaceae. J Arnold Arbor, 71(3): 323-370.
• Celep F, Dirmenci T. 2017. Systematic and biogeographic overview of Lamiaceae in Turkey. Nat Vol Essent Oil, 4(4): 14-27.
• Corsi G, Bottega S. 1999. Glandular hairs of Salvia officinalis: new data on morphology, localization and histocemistry in relation to function. Ann Bot, 84: 657-664.
• Cullen J. 1982. Marrubium L. In: Davis PH, Edmondson JR, Mill RR, Tan K, editors. Flora of Turkey and the East Aegean Islands. Edinburgh University Press, Edinburg, 7th, pp: 165-178.
• Çalı İÖ. 2017. Glandular trichomes in Marrubium cephalanthum ssp. montanum (Lamiaceae) growing in Turkey. Int J Agric and Biol, 19(4): 697-700.
• Çelebioğlu S, Baytop T. 1949. A new reagent for microscopical investigation of plant. Publication of the Institute of Pharmacognosy, İstanbul, Türkiye, pp: 301.
• Dmitruk M, Haratym W. 2014. Morphological differentiation of non-glandular and glandular trichomes on Marrubium vulgare L. Mod Phytomorphol, 6: 85.
• El-Deen Osman AK. 2012. Trichome micromorphology of Egyptian Ballota L. (Lamiaceae) with emphasis on its systematic implication. Pak J Bot, 44(1): 33-46.
• Gyuzeleva DP, Stoyanov P, Bivolarska AV, Mladenov RD, Mladenova TR, Petkov VH, Todorov KT. 2022. Anatomical investigation of Marrubium friwaldskyanum Boiss. and Marrubium peregrinum L. (Lamiaceae) from Bulgaria. Ecol Balk, 14(1): 87-101.
• Haratym W, Weryszko-Chmielewska E. 2017. Ultrastructural and histochemical analysis of glandular trichomes of Marrubium vulgare L. (Lamiaceae). Flora, 231: 11-20.
• Hatamneia AA, Khayami M, Mahmudzadeh A, Sarghein SH, Heidarih M. 2008. Comparative anatomical studies of some genera of Lamiaceae family in West Azarbaijan in Iran. Bot Res J, 1(3):63-67.
• Inyama C, Mbagwu F, Duru C. 2016. Taxonomic relationship on some Chrysophyllum species based on anatomical studies. Med Aromat Plants, 5(2):1-5.
• Kaçar B. 1995. Chemical analysis of plants and soil. Ankara University Faculty of Agriculture Education, Research and Development Foundation Publications, Ankara, Türkiye, pp: 705.
• Kamatou GP, Van Z, Van-Vuuren RL, Viljoen SF, Figueiredo AM, Barroso AC, Pedro JG, Tilney PM. 2006. Chemical composition, leaf trichome types and biological activities of the essensial oils of four related Salvia species indigenous to Southern Africa. J Essent Oil Res, 18:72-79.
• Kandemir N. 2003. The Morphological anatomical and karyological properties of endemic Salvia hypargeia fich. & mey. (lamiaceae) in Turkey. Pak J Bot, 35 (2): 219-236.
• Kandemir N. 2011. Trichomes on Salvia pomifera (Lamiaceae) in Turkey, Bot Lith, 17(1): 3-11.
• Kandemir, N. 2019. Comparative morphological and anatomical studies on Iris peshmeniana Güner & T. Hall. and Iris aucheri (Baker) Sealy (Iridaceae). Trak Univ J Nat Sci, 20(2): 105-113.
• Kandemir N, Çelik A, Ullah F, Shah SN, Zaman W. 2019. Foliar epidermal anatomical characteristics of taxa of Iris subg. Scorpiris Spach (Iridaceae) from Turkey, Microsc Res Tech, 82(6): 764–774
• Kandemir N, Çelik A, Shah SN, Razzaq A. 2020. Comparative micro-anatomical investigation of genus Heliotropium (Boraginaceae) found in Turkey. Flora, 262: 151495.
• Kandemir N, Çelik A, Ullah F. 2022. Comparative micro-anatomical features of endemic Fritillaria taxa growing in the Mediterranean region (Turkey). Flora, 290 (2): 152049.
• Koçak KV, Kandemir N. 2023. Taxonomic importance of anatomic, ecological and trichome features of Marrubium astracanicum subsp. astracanicum Jacq. (Lamiaceae). BSJ Eng Sci, 6 (3): 199-209.
• Metcalfe CR, Chalk L. 1972. Anatomy of the dicotyledons. Oxford University Press, Oxford, UK, pp: 1459.
• Metcalfe CR, Chalk L. 1979. Anatomy of the dicotyledons. Oxford University press, Oxford, UK, 2nd, pp: 276.
• Meyre-Silva CH, Cechinel-Filho V. 2010. A review of the chemical and pharmacological aspects of the genus Marrubium. Curr Pharm Des, 16: 3503-3518.
• Navarro T, El Oualidi, J. 2000. Trichome morphology in Teucrium L. (Labiatae). A taxonomic review. An Jard Bot Madr, 57(2): 277-297.
• Okur ME, Karakaş N, Karadağ AE, Yılmaz R, Demirci F. 2019. In vitro cytotoxicity evaluation of Marrubium vulgare L. methanol extract. J Res Pharm, 23(4): 711-718.
• Ryding O. 2010. Crystals in calyces of Lamiaceae and their phylogenetic and adaptive significance. Plant Syst Evol, 290:201-215.
• Sargın SA, Selvi S, López V. 2015. Ethnomedicinal plants of Sarigöl district (Manisa), Turkey. J Ethnopharmacol, 171: 64-84.
• Selvi S, Polat R, Çakılcıoğlu U, Celep F, Dirmenci T, Ertuğ ZF. 2022. An ethnobotanical review on medicinal plants of the Lamiaceae family in Turkey. Turk J Bot, 46(4): 283-332.
• Serrato-Valanti G, Bisio A, Cornara L, Ciarallo G 1997. Structural and histochemical investigation of the glandular trichomes of Salvia aurea L. leaves and chemical analysis of the essential oil Ann Bot, 79: 329-336.
• Seyedi Z, Salmaki Y. 2015. Trichome morphology and its significance in the systematic of Phlomoides (Lamiaceae; Lamioideae; Phlomideae). Flora, 213: 40-48.
• Shahri S, Jafari A, Mahmoodzadeh H. 2016. Comparative anatomical studies on petioles of Nepeta L. Species (Lamiaceae) in NE Iran. Adv Stud Biol, 8(3): 119-126.
• Talebi S, Sheidai M, Ariyanejad F, Matsyura A. 2019a. Stem anatomical study of some Iranian Marrubium L. species. Biodiversitas, 20(9): 2589-2595.
• Talebi SM, Sheidai M, Ariyanejad F. 2019b. Investigation of leaf anatomical structure variation among different populations of Marrubium anisodon C. Koch in Iran. J Cell Tiss, 9(4): 388-397.
• Tuylu M, Büyükkartal HN, Akgül G, Kalyoncu H. 2017. Comparing stem and leaf anatomy of Marrubium lutescens Boiss. and M. cephalanthum Boiss. & Noë subsp. akdaghicum (Lamiaceae). SDU J Nat Appl Sci, 21(1): 113-117.
• Tuzlacı E. 2016. Traditional medicinal plants of Turkey. Istanbul Medical Bookstores, İstanbul, Türkiye, pp: 286.
• Xiang C, Dong Z, Peng H, Lui Z. 2010. Trichome micromorphology of the East Asiatic genus Chelonopsis (Lamiaceae) and its systematic implications. Flora, 205: 434-441.
• Yousefi K, Fathiazad F, Soraya H, Rameshrad M, Maleki-Dizaji N, Garjani A. 2014. Marrubium vulgare L. methanolic extract inhibits inflammatory response and prevents cardiomyocyte fibrosis in isoproterenol-induced acute myocardial infarction in rats. Bioimpacts, 4(1): 21-27.