Highly Up-Regulation of Fas Ligand Gene Expression after Increasing in Oxidized Low-Density Lipoprotein

Year 2025, Volume: 8 Issue: 1, 1 - 2

Burcu Bayyurt , Serdal Arslan

https://doi.org/10.34248/bsengineering.1578992

Abstract

Oxidized low-density lipoprotein (ox-LDL) shows many harmful effects such as induction of apoptosis on function of endothelial cell (EC). Fas ligand (FASL) induces apoptosis in divergent pathological conditions. EC apoptosis which induced by ox-LDL is related with FASL. In this study, we aimed to determine how different ox-LDL levels affect FASL expression in ECs. We treated human umbilical vein endothelial cells (HUVECs) with two different concentration of ox-LDL. FASL gene expression in groups was detected by quantitative polymerase chain reaction (qPCR). We compared FASL gene expression level between the groups according to ∆∆Ct method. FASL gene expression was statistic significantly up-regulated in the group treated higher amounts of ox-LDL (P<0.001). We found that treatment with higher amounts of ox-LDL in HUVECs increased FASL gene expression dramatically. According to the our findings, we concluded that increasing the amount of ox-LDL may be critical in inducing the FASL-dependent apoptotic pathway in ECs.

Keywords

Oxidized low-density lipoprotein, Gene expression, Quantitative polymerase chain reaction, Human umbilical vein endothelial cell, Fas ligand

Ethical Statement

Ethics committee approval was not required for this study because of there was no study on animals or humans.

Thanks

HUVEC line and ox-LDL used in cell line experiments were provided by Sivas Cumhuriyet University Scientific Research Unit (CUBAP) (project no.: T-893) and Scientific Research and Technological Research Council of Turkey (TUBITAK) (project no.: 218S524), respectively.

Highly Up-Regulation of Fas Ligand Gene Expression after Increasing in Oxidized Low-Density Lipoprotein

Abstract

Oksitlenmiş düşük yoğunluklu lipoprotein (ox-LDL), endotel hücresi (EC) fonksiyonu üzerinde apoptozis indüksiyonu gibi birçok zararlı etki gösterir. Fas ligand (FASL), farklı patolojik koşullarda apoptozu indükler. Ox-LDL tarafından indüklenen EC apoptozu FASL ile ilişkilidir. Bu çalışmada, farklı ox-LDL düzeylerinin EC'lerde FASL ekspresyonunu nasıl etkilediğini belirlemeyi amaçladık. İnsan göbek kordonu ven endotel hücrelerini (HUVEC'leri) iki farklı ox-LDL konsantrasyonu ile muamele ettik. Gruplardaki FASL gen ekspresyonu kantitatif polimeraz zincir reaksiyonu (qPCR) ile tespit edildi. Gruplar arasındaki FASL gen ekspresyon düzeyini ∆∆Ct yöntemine göre karşılaştırdık. FASL gen ekspresyonu, daha yüksek miktarda ox-LDL ile muamele edilen grupta istatistiki olarak anlamlı şekilde yukarı regüle edildi (P<0,001). HUVEC'lerde daha yüksek miktarda ox-LDL ile muamelenin FASL gen ekspresyonunu önemli ölçüde artırdığını bulduk. Bulgularımıza göre, EC'lerde FASL'ye bağlı apoptotik yolağın indüklenmesinde ox-LDL miktarının artırılmasının kritik olabileceği sonucuna vardık.

Keywords

Fas ligand, Oksitlenmiş düşük yoğunluklu lipoprotein, Gen ifadesi, Kantitatif polimeraz zincir reaksiyonu, İnsan göbek kordonu ven endotel hücresi

References

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