Year 2020, Volume 3 , Issue 3, Pages 51 - 62 2020-09-01

Assessment of the Antimicrobial Effectiveness of Hypericum Perforatum L. and Secondary Metabolites against Pathogenic Oral Bacteria

Feride BALLI [1] , Ayça Tuba ULUSOY YAMAK [2]


Amaç: Bu çalışmanın amacı, Hypericum perforatum L.’nin ve içindeki sekonder metabolitlerin, diş çürüğü ve periodontal hastalıklardan sorumlu mikroorganizmalar üzerine antimikrobiyal etkisini araştırarak, bu bitkinin ve sekonder metabolitlerinin insan gingival fibroblast hücreleri üzerine sitotoksisitelerini in vitro olarak incelemektir. Materyal ve Metot: Çalışmamızda Hypericum perforatum L. bitki ekstratı ve 6 ayrı sekonder metabolit (hyperforin, hypericin, hyperoside, quercetin, quercitrin, rutin) kullanıldı. Bu ürünlerin patojenik oral mikroorganizmalara [S. mutans, S. mitis, S. sanguinis, S. salivarius, S. sobrinus, L. acidophilus, P. gingivalis, A. actinomycetemcomitans, C. albicans] karşı antimikrobiyal aktivitesi disk difüzyon yöntemi ile incelenirken, MIC ve MBC değerleri de belirlendi. Tüm test materyallerinin oral dokular üzerine sitotoksisitesi, insan gingival fibroblast hücreleri kullanılarak MTT analizi ile değerlendirildi. Bulgular: Hypericum perforatum L. ekstratı ve sekonder metabolitlerinin çalışmamızda test edilen tüm oral patojenlere etkisi istatistiki olarak CHX’den düşük bulunmuştur. Bununla birlikte, periodontal hastalıkla ilişkili gram-negatif mikroorganizmalara ve C. albicans’a karşı CHX’ne yakın değerde inhibisyon zonu oluşturduğu görülen quercitrin’in, gingival fibroblast hücreleri üzerine CHX gibi şiddetli sitotoksisite göstermediği tespit edilmiştir. Sonuç: Bu çalışmada, bitki ekstratlarının saf bileşikler olmaması nedeniyle bu yönde yapılacak çalışmaların sadece bitkileri değil sekonder metabolitleri de incelemesi, yüksek antimikrobiyal etkinliğe sahip, düşük sitotoksik etkili yeni antimikrobiyal ve antifungal bitki ekstratları ve sekonder metabolitlerin bulunmasını mümkün kılacağı sonucuna varılmıştır.
Diş çürüğü, doğal antimikrobiyal ajanlar, hyperforin, hypericum peroratum L., hyperoside, quercitrin
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Primary Language tr
Subjects Dental
Journal Section Research Articles
Authors

Orcid: 0000-0000-0000-0000
Author: Feride BALLI
Institution: ZONGULDAK AĞIZ VE DİŞ SAĞLIĞI MERKEZİ
Country: Turkey


Orcid: 0000-0000-0000-0002
Author: Ayça Tuba ULUSOY YAMAK (Primary Author)
Institution: ONDOKUZ MAYIS ÜNİVERSİTESİ, DİŞ HEKİMLİĞİ FAKÜLTESİ
Country: Turkey


Dates

Publication Date : September 1, 2020

Bibtex @research article { bshealthscience746614, journal = {Black Sea Journal of Health Science}, issn = {}, eissn = {2619-9041}, address = {bsjhealthsci@blackseapublishers.com}, publisher = {Cem TIRINK}, year = {2020}, volume = {3}, pages = {51 - 62}, doi = {}, title = {Assessment of the Antimicrobial Effectiveness of Hypericum Perforatum L. and Secondary Metabolites against Pathogenic Oral Bacteria}, key = {cite}, author = {Ballı, Feride and Ulusoy Yamak, Ayça Tuba} }
APA Ballı, F , Ulusoy Yamak, A . (2020). Assessment of the Antimicrobial Effectiveness of Hypericum Perforatum L. and Secondary Metabolites against Pathogenic Oral Bacteria . Black Sea Journal of Health Science , 3 (3) , 51-62 . Retrieved from https://dergipark.org.tr/en/pub/bshealthscience/issue/55047/746614
MLA Ballı, F , Ulusoy Yamak, A . "Assessment of the Antimicrobial Effectiveness of Hypericum Perforatum L. and Secondary Metabolites against Pathogenic Oral Bacteria" . Black Sea Journal of Health Science 3 (2020 ): 51-62 <https://dergipark.org.tr/en/pub/bshealthscience/issue/55047/746614>
Chicago Ballı, F , Ulusoy Yamak, A . "Assessment of the Antimicrobial Effectiveness of Hypericum Perforatum L. and Secondary Metabolites against Pathogenic Oral Bacteria". Black Sea Journal of Health Science 3 (2020 ): 51-62
RIS TY - JOUR T1 - Assessment of the Antimicrobial Effectiveness of Hypericum Perforatum L. and Secondary Metabolites against Pathogenic Oral Bacteria AU - Feride Ballı , Ayça Tuba Ulusoy Yamak Y1 - 2020 PY - 2020 N1 - DO - T2 - Black Sea Journal of Health Science JF - Journal JO - JOR SP - 51 EP - 62 VL - 3 IS - 3 SN - -2619-9041 M3 - UR - Y2 - 2020 ER -
EndNote %0 Black Sea Journal of Health Science Assessment of the Antimicrobial Effectiveness of Hypericum Perforatum L. and Secondary Metabolites against Pathogenic Oral Bacteria %A Feride Ballı , Ayça Tuba Ulusoy Yamak %T Assessment of the Antimicrobial Effectiveness of Hypericum Perforatum L. and Secondary Metabolites against Pathogenic Oral Bacteria %D 2020 %J Black Sea Journal of Health Science %P -2619-9041 %V 3 %N 3 %R %U
ISNAD Ballı, Feride , Ulusoy Yamak, Ayça Tuba . "Assessment of the Antimicrobial Effectiveness of Hypericum Perforatum L. and Secondary Metabolites against Pathogenic Oral Bacteria". Black Sea Journal of Health Science 3 / 3 (September 2020): 51-62 .
AMA Ballı F , Ulusoy Yamak A . Assessment of the Antimicrobial Effectiveness of Hypericum Perforatum L. and Secondary Metabolites against Pathogenic Oral Bacteria. BSJ Health Sci.. 2020; 3(3): 51-62.
Vancouver Ballı F , Ulusoy Yamak A . Assessment of the Antimicrobial Effectiveness of Hypericum Perforatum L. and Secondary Metabolites against Pathogenic Oral Bacteria. Black Sea Journal of Health Science. 2020; 3(3): 51-62.