Research Article
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Determination of Antimicrobial Activity of Various Plant Essential Oils on Vancomycin Resistant Enterococci (VRE) and Some Pathogenic Microorganisms

Year 2021, Volume: 4 Issue: 1, 26 - 34, 01.01.2021
https://doi.org/10.19127/bshealthscience.792782

Abstract

In this study, it was aimed to determine the anti-bacterial activities of plant essential oils such as thyme, rosemary, sage, linden, black seed, bay leaf, peppermint, parsley, cinnamon and oleagen on vancomycin resistant enterococci (VRE) and pathogenic microorganisms of clinical importance. In the study, the inhibition zones of the plant extract of 10 plants prepared at 2.5 and 5 mg / ml
concentrations of VRE and various pathogenic microorganisms were detected by Disk Diffusion and Agar Well Diffusion Method. Following this, Minimal Inhibition Concentration (MIC) values of these extracts were determined. The activities of plant extracts on DNA and Gas Chromatography-Mass Spectrometry (GC-MS) analyzes were also performed. According to the data obtained, it was determined that the extracts of rosemary and sage plants showed high antibacterial effect against pathogenic bacteria. The rosemary extract was found to be the most effective extract on VRE strains, as well as medium-grade antibacterial effects on the VRE strains in oleaster, linden, thyme and mint extracts. It was also determined that extracts from different plants did not cause any change in the structure of DNA. In our study, it was determined that plant extracts that are especially effective on VRE strains have the potential to be used in the treatment of VRE. The results of this study should be taken into account in the research of drugs that can be used in the treatment of VRE and more in vitro and in vivo research should be carried out in this field.

Supporting Institution

Kırşehir Ahi Evran University Scientific Research Projects

Project Number

TIP.A4.07005.

Thanks

This research was supported by Kirsehir Ahi Evran University Scientific Research Projects TIP.A4.07005

References

  • Akgül Y. 2014. İdrar yolu enfeksiyonlarına neden olan pseudomonas aeruginosa ve klebsiella pneumoniae izolatlarına karşı bazı bitki ekstraktlarının antibakteriyel aktiviteleri. Yüksek Lisans Tezi, Nevşehir Hacı Bektaş Veli Üniversitesi Fen Bilimleri Enstitüsü, Nevşehir, pp. 57-58.
  • Asgin N, Otlu B. 2020. Antibiotic resistance and molecular epidemiology of vancomycin-resistant enterococci in a tertiary care hospital in Turkey. Infect Drug Resist, 13:191–198.
  • Berzeg D. 2005. Çeşitli klinik materyallerden izole edilen enterokok suşlarında antibiyotik direnci, yüksek düzey aminoglikozid direnci ve E test ile vankomisin mik değerlerinin değerlendirilmesi. İstanbul: Haseki Eğitim ve Araştırma Hastanesi İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Kliniği.
  • Burt S. 2004. Essential oils: their antibacterial properties and potential applications in foods-a review. Inter J Food Microbiol, 94: 223-253.
  • Correa-Martinez CL, Tönnies H, Froböse NJ, Mellmann A, Kampmeier S. 2020. Transmission of vancomycin-resistant enterococci in the hospital setting: uncovering the patient-environment interplay. Microorganisms. 31: 8(2), 203.
  • Çaylan R, Üstünakın M, Kadimov V, Aydın K, Köksal İ. 2004 Fekal ve klinik örneklerden izole edilen enterokok suşlarının antibiyotiklere duyarlılıkları. Türk Mikrobiyol Cemiyeti Derg, 34: 24-28.
  • Delamare APL, Moschen-Pistorello IT, Artico L, Atti-Serafini L, Echeverrigaray S. 2007. Antibacterial activity of the essential oils Salvia officinalis L. and Salvia triloba L. cultivated in South Brazil. Food Chem, 100: 603-608.
  • Djabou N, Lorenzi V, Guinoiseau E. 2013. Phytochemical composition of Corsican Teucrium essential oils and antibacterial activity against foodborne or toxi-infectious pathogens. Food Control, 354-363.
  • Ertürk R, Çelik C, Kaygusuz R, Aydın H. 2010. Ticari olarak satılan kekik ve nane uçucu yağlarının antimikrobiyal aktiviteleri. Cumhuriyet Tıp Dergisi, 32: 281-286.
  • Evren M. 2011. Uçucu Yağların antimikrobiyel özellikleri. Elektronik Mikrobiyol Derg, 09(3): 28-40.
  • Fani M, Kohanteb J. 2017. In vitro antimicrobial activity of Thymus vulgaris essential oil against major oral pathogens. J. Evid. Based Complem Altern Med, 22(4):660-666.
  • Faydaoğlu E, Sürücüoğlu MS. 2013. Tıbbi ve aromatik bitkilerin antimikrobiyal, antioksidan aktiviteleri ve kullanım olanakları. Ankara Üniversitesi Sağlık Bilimleri Fakültesi Beslenme ve Diyetetik Bölümü, Fen Bilimleri Enstitüsü Dergisi, 6(2), 233-265.
  • Hamoud R, Sporer F, Reichling Wink M. 2012. Antimicrobial activity of a traditionally used complex essential oil distillate (Olbas® Tropfen) in comparison to its individual essential oil ingredients. Phytomedicine, 19: 969–976.
  • Kalemba D, Kunicka A. 2003. Antibacterial and antifungal properties of essential oils. Current medicinal chemistry,10, 813-829.
  • Karagöz GD. 2005. Dr. Lütfi kırdar kartal eğitim ve araştırma hastanesi infeksiyon hastalıkları ve klinik mikrobiyoloji kliniği yoğun bakım ünitesinde vankomisin dirençli enterokok taşıyıcılığının araştırılması. pp. 5-65.
  • Lautenbach E, Bilker W, Brennan P. 1999. Enterococcal bacteremia: Risk factors for vancomycin resistance and predictors of mortality. Infect Control Hospital Epid, 20: 318.
  • Marchese A, Orhan IE, Daglia M, Barbieri R, Di Lorenzo A, Nabavi SF, Gortzi O, Izadi M, Nabavi SM. 2016. Antibacterial and antifungal activities of thymol: A brief review of the literature. Food Chem, 210: 402–414.
  • Ouattara B, Simard RE, Holley RA, Piette GJP, Bégin A. 1997. Antimicrobial activity of selected fatty acids and essential oils against six meat spoilage organisms. Inter J Food Microbiol, 37: 155-162.
  • Patterson J. 2000. New Gram-positive agents in nosocomial infection. Current Opinion in Infectious Diseases, 13: 593.
  • Reyes K, Bardossy AC, Zervos M. 2016. Vankomisine dirençli enterokoklar: epidemiyoloji, enfeksiyon önleme ve kontrol. Infect Dis Clin Kuzey Am, 30(4): 953-965.
  • Roller S, Ernest N, Buckle J. 2009. The antimicrobial activity of high-necrodane and other lavender oils on methicillin-sensitive and -resistant Staphylococcus aureus (MSSA and MRSA). J Altern Complement Med, 15: 275–279.
  • Sağdıç O, Özcan M. 2003. Antibacterial activity of Turkish spice hydrosols. Food Control, 14: 141-143.
  • Salehi B, Abu-Darwish MS, Tarawneh AH, et al. 2019. Thymus spp. plants - Food applications and phytopharmacy properties. Trends Food Sci Technol, 85: 286–306.
  • Selen İşbilir Ş. 2008. Yaprakları salata-baharat olarak tüketilen bazı bitkilerin antioksidan aktivitelerinin incelenmesi. Doktora Tezi, Trakya Üniversitesi, Fen Bilimleri Enstitüsü, Edirne, pp. 91-104.
  • Selim S. 2011. Antimicrobial activity of essential oils against vancomycin-resistant enterococci (vre) and Escherichia coli o157:h7 in feta soft cheese and minced beef meat. Braz J Microbiol, 42(1): 187-96.
  • Sharma SK, Guleria S. 2017. Antimicrobial activity of some essential oils-present status and future perspectives. Medicines, 4: 58.
  • Shepard BD, Gilmore MS. 2002. Antibiotic resi,stant enterococci: the mechanisms and Dynamics of drug introduction and resistance. Microbes Infection, 4(2): 215-24
  • Toroğlu S, Dıgrak M, Çenet M. 2006. Baharat olarak tüketilen Laurus nobilis Linn ve Zingiber officinale Roscoe bitki uçucu yaglarının antimikrobiyel aktiviteleri ve antibiyotiklere in-vitro etkilerinin belirlenmesi, KSÜ Fen ve Mühendislik Derg, 9(1), 20.
  • Uçan F. 2008. DL-Limonenin mayalar üzerine antifungal etkisi. Yüksek lisans tezi, Çukurova Üniversitesi, Fen Bilimleri Enstitüsü, Adana, pp 62.
  • Wińska K, Mączka W, Łyczko J, Grabarczyk M, Czubaszek A, Szumny A. 2019. Essential oils as antimicrobial agents—myth or real alternative? Molecules, 24(11): 2130.
Year 2021, Volume: 4 Issue: 1, 26 - 34, 01.01.2021
https://doi.org/10.19127/bshealthscience.792782

Abstract

Project Number

TIP.A4.07005.

References

  • Akgül Y. 2014. İdrar yolu enfeksiyonlarına neden olan pseudomonas aeruginosa ve klebsiella pneumoniae izolatlarına karşı bazı bitki ekstraktlarının antibakteriyel aktiviteleri. Yüksek Lisans Tezi, Nevşehir Hacı Bektaş Veli Üniversitesi Fen Bilimleri Enstitüsü, Nevşehir, pp. 57-58.
  • Asgin N, Otlu B. 2020. Antibiotic resistance and molecular epidemiology of vancomycin-resistant enterococci in a tertiary care hospital in Turkey. Infect Drug Resist, 13:191–198.
  • Berzeg D. 2005. Çeşitli klinik materyallerden izole edilen enterokok suşlarında antibiyotik direnci, yüksek düzey aminoglikozid direnci ve E test ile vankomisin mik değerlerinin değerlendirilmesi. İstanbul: Haseki Eğitim ve Araştırma Hastanesi İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Kliniği.
  • Burt S. 2004. Essential oils: their antibacterial properties and potential applications in foods-a review. Inter J Food Microbiol, 94: 223-253.
  • Correa-Martinez CL, Tönnies H, Froböse NJ, Mellmann A, Kampmeier S. 2020. Transmission of vancomycin-resistant enterococci in the hospital setting: uncovering the patient-environment interplay. Microorganisms. 31: 8(2), 203.
  • Çaylan R, Üstünakın M, Kadimov V, Aydın K, Köksal İ. 2004 Fekal ve klinik örneklerden izole edilen enterokok suşlarının antibiyotiklere duyarlılıkları. Türk Mikrobiyol Cemiyeti Derg, 34: 24-28.
  • Delamare APL, Moschen-Pistorello IT, Artico L, Atti-Serafini L, Echeverrigaray S. 2007. Antibacterial activity of the essential oils Salvia officinalis L. and Salvia triloba L. cultivated in South Brazil. Food Chem, 100: 603-608.
  • Djabou N, Lorenzi V, Guinoiseau E. 2013. Phytochemical composition of Corsican Teucrium essential oils and antibacterial activity against foodborne or toxi-infectious pathogens. Food Control, 354-363.
  • Ertürk R, Çelik C, Kaygusuz R, Aydın H. 2010. Ticari olarak satılan kekik ve nane uçucu yağlarının antimikrobiyal aktiviteleri. Cumhuriyet Tıp Dergisi, 32: 281-286.
  • Evren M. 2011. Uçucu Yağların antimikrobiyel özellikleri. Elektronik Mikrobiyol Derg, 09(3): 28-40.
  • Fani M, Kohanteb J. 2017. In vitro antimicrobial activity of Thymus vulgaris essential oil against major oral pathogens. J. Evid. Based Complem Altern Med, 22(4):660-666.
  • Faydaoğlu E, Sürücüoğlu MS. 2013. Tıbbi ve aromatik bitkilerin antimikrobiyal, antioksidan aktiviteleri ve kullanım olanakları. Ankara Üniversitesi Sağlık Bilimleri Fakültesi Beslenme ve Diyetetik Bölümü, Fen Bilimleri Enstitüsü Dergisi, 6(2), 233-265.
  • Hamoud R, Sporer F, Reichling Wink M. 2012. Antimicrobial activity of a traditionally used complex essential oil distillate (Olbas® Tropfen) in comparison to its individual essential oil ingredients. Phytomedicine, 19: 969–976.
  • Kalemba D, Kunicka A. 2003. Antibacterial and antifungal properties of essential oils. Current medicinal chemistry,10, 813-829.
  • Karagöz GD. 2005. Dr. Lütfi kırdar kartal eğitim ve araştırma hastanesi infeksiyon hastalıkları ve klinik mikrobiyoloji kliniği yoğun bakım ünitesinde vankomisin dirençli enterokok taşıyıcılığının araştırılması. pp. 5-65.
  • Lautenbach E, Bilker W, Brennan P. 1999. Enterococcal bacteremia: Risk factors for vancomycin resistance and predictors of mortality. Infect Control Hospital Epid, 20: 318.
  • Marchese A, Orhan IE, Daglia M, Barbieri R, Di Lorenzo A, Nabavi SF, Gortzi O, Izadi M, Nabavi SM. 2016. Antibacterial and antifungal activities of thymol: A brief review of the literature. Food Chem, 210: 402–414.
  • Ouattara B, Simard RE, Holley RA, Piette GJP, Bégin A. 1997. Antimicrobial activity of selected fatty acids and essential oils against six meat spoilage organisms. Inter J Food Microbiol, 37: 155-162.
  • Patterson J. 2000. New Gram-positive agents in nosocomial infection. Current Opinion in Infectious Diseases, 13: 593.
  • Reyes K, Bardossy AC, Zervos M. 2016. Vankomisine dirençli enterokoklar: epidemiyoloji, enfeksiyon önleme ve kontrol. Infect Dis Clin Kuzey Am, 30(4): 953-965.
  • Roller S, Ernest N, Buckle J. 2009. The antimicrobial activity of high-necrodane and other lavender oils on methicillin-sensitive and -resistant Staphylococcus aureus (MSSA and MRSA). J Altern Complement Med, 15: 275–279.
  • Sağdıç O, Özcan M. 2003. Antibacterial activity of Turkish spice hydrosols. Food Control, 14: 141-143.
  • Salehi B, Abu-Darwish MS, Tarawneh AH, et al. 2019. Thymus spp. plants - Food applications and phytopharmacy properties. Trends Food Sci Technol, 85: 286–306.
  • Selen İşbilir Ş. 2008. Yaprakları salata-baharat olarak tüketilen bazı bitkilerin antioksidan aktivitelerinin incelenmesi. Doktora Tezi, Trakya Üniversitesi, Fen Bilimleri Enstitüsü, Edirne, pp. 91-104.
  • Selim S. 2011. Antimicrobial activity of essential oils against vancomycin-resistant enterococci (vre) and Escherichia coli o157:h7 in feta soft cheese and minced beef meat. Braz J Microbiol, 42(1): 187-96.
  • Sharma SK, Guleria S. 2017. Antimicrobial activity of some essential oils-present status and future perspectives. Medicines, 4: 58.
  • Shepard BD, Gilmore MS. 2002. Antibiotic resi,stant enterococci: the mechanisms and Dynamics of drug introduction and resistance. Microbes Infection, 4(2): 215-24
  • Toroğlu S, Dıgrak M, Çenet M. 2006. Baharat olarak tüketilen Laurus nobilis Linn ve Zingiber officinale Roscoe bitki uçucu yaglarının antimikrobiyel aktiviteleri ve antibiyotiklere in-vitro etkilerinin belirlenmesi, KSÜ Fen ve Mühendislik Derg, 9(1), 20.
  • Uçan F. 2008. DL-Limonenin mayalar üzerine antifungal etkisi. Yüksek lisans tezi, Çukurova Üniversitesi, Fen Bilimleri Enstitüsü, Adana, pp 62.
  • Wińska K, Mączka W, Łyczko J, Grabarczyk M, Czubaszek A, Szumny A. 2019. Essential oils as antimicrobial agents—myth or real alternative? Molecules, 24(11): 2130.
There are 30 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Research Article
Authors

Revza Çelik 0000-0002-6733-617X

Esin Kıray 0000-0002-6908-5909

Ergin Kariptaş 0000-0001-6513-9589

Project Number TIP.A4.07005.
Publication Date January 1, 2021
Submission Date September 9, 2020
Acceptance Date November 6, 2020
Published in Issue Year 2021 Volume: 4 Issue: 1

Cite

APA Çelik, R., Kıray, E., & Kariptaş, E. (2021). Determination of Antimicrobial Activity of Various Plant Essential Oils on Vancomycin Resistant Enterococci (VRE) and Some Pathogenic Microorganisms. Black Sea Journal of Health Science, 4(1), 26-34. https://doi.org/10.19127/bshealthscience.792782
AMA Çelik R, Kıray E, Kariptaş E. Determination of Antimicrobial Activity of Various Plant Essential Oils on Vancomycin Resistant Enterococci (VRE) and Some Pathogenic Microorganisms. BSJ Health Sci. January 2021;4(1):26-34. doi:10.19127/bshealthscience.792782
Chicago Çelik, Revza, Esin Kıray, and Ergin Kariptaş. “Determination of Antimicrobial Activity of Various Plant Essential Oils on Vancomycin Resistant Enterococci (VRE) and Some Pathogenic Microorganisms”. Black Sea Journal of Health Science 4, no. 1 (January 2021): 26-34. https://doi.org/10.19127/bshealthscience.792782.
EndNote Çelik R, Kıray E, Kariptaş E (January 1, 2021) Determination of Antimicrobial Activity of Various Plant Essential Oils on Vancomycin Resistant Enterococci (VRE) and Some Pathogenic Microorganisms. Black Sea Journal of Health Science 4 1 26–34.
IEEE R. Çelik, E. Kıray, and E. Kariptaş, “Determination of Antimicrobial Activity of Various Plant Essential Oils on Vancomycin Resistant Enterococci (VRE) and Some Pathogenic Microorganisms”, BSJ Health Sci., vol. 4, no. 1, pp. 26–34, 2021, doi: 10.19127/bshealthscience.792782.
ISNAD Çelik, Revza et al. “Determination of Antimicrobial Activity of Various Plant Essential Oils on Vancomycin Resistant Enterococci (VRE) and Some Pathogenic Microorganisms”. Black Sea Journal of Health Science 4/1 (January 2021), 26-34. https://doi.org/10.19127/bshealthscience.792782.
JAMA Çelik R, Kıray E, Kariptaş E. Determination of Antimicrobial Activity of Various Plant Essential Oils on Vancomycin Resistant Enterococci (VRE) and Some Pathogenic Microorganisms. BSJ Health Sci. 2021;4:26–34.
MLA Çelik, Revza et al. “Determination of Antimicrobial Activity of Various Plant Essential Oils on Vancomycin Resistant Enterococci (VRE) and Some Pathogenic Microorganisms”. Black Sea Journal of Health Science, vol. 4, no. 1, 2021, pp. 26-34, doi:10.19127/bshealthscience.792782.
Vancouver Çelik R, Kıray E, Kariptaş E. Determination of Antimicrobial Activity of Various Plant Essential Oils on Vancomycin Resistant Enterococci (VRE) and Some Pathogenic Microorganisms. BSJ Health Sci. 2021;4(1):26-34.