Subcutaneous Connective Tissue Reactions to New Calcium Silicate Cements: An Animal Study

Year 2021, Volume: 4 Issue: 3, 213 - 220, 01.09.2021

Seçil Çalışkan Nuray Tüloğlu Barış Karabulut Ceren Canbey Şule Bayrak

https://doi.org/10.19127/bshealthscience.889531

Abstract

The aim of this study was to determine the subcutaneous connective tissue reactions to Medcem MTA and Medcem Pure Portland Cement. Medcem MTA, Medcem Pure Portland Cement and ProRoot MTA were placed in polyethylene tubes and implanted into the dorsal connective tissue of Sprague Dawley rats. The presence of inflammation, edema, necrosis, dystrophic calcification, and thickness of fibrous capsule formation was recorded by histological examination 7, 30, and 60 days after the implantation procedure. Inflammation scores were defined as follows: 0 = no or few inflammatory cells, no reaction, 1 = <25 cells, mild reaction; 2 = 25 to 125 cells, moderate reaction; and 3 = ≥125 cells, severe reaction. Fibrous capsule thickness, necrosis, and formation of calcification were recorded. The Kruskal–Wallis test and repeated measures analysis of variance were used for statistically analyses (P < 0.05). No significant differences in edema, necrosis and fibrous capsule formation were observed between the groups on any of the three euthanasia days. All experimental groups exhibited significantly more inflammation than the control group. On Day 30 and 60, all experimental groups exhibited significantly more dystrophic calcification than the control group. Medcem MTA and Medcem Pure Portland Cement had similar biocompatibility to ProRoot MTA. Medcem MTA and Medcem Pure Portland Cement with the presence of dystrophic calcification in connective tissue have the potential to be clinic use as calcium silicate materials.

Keywords

Biocompatible Materials, Dental material, Mineral trioxide aggregate

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