Research Article
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The Impact of Endemic Iris taochia Ethanolic Extracts on Human Lung Adenocarcinoma Cells

Year 2022, Volume 5, Issue 3, 395 - 403, 01.09.2022
https://doi.org/10.19127/bshealthscience.1100668

Abstract

Iris taochia is an elegant endemic plant in Türkiye and it has limited distribution. In this study, cytotoxic effects of ethanolic extracts from different parts and concentrations extracted from I. taochia collected from the surroundings of Tortum (Erzurum), on A549 human lung adenocarcinoma cell line were investigated. Cytotoxicity of extracts were evaluated by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) method. Apopototic activity of IC50 values of extracts were evaluated with Annexin V and Caspase 3/7 assays. Ultrastructural changes of IC50 doses treated cells were investigated by transmission electron microscopy. As a result, it was determined that ethanol extract of I. taochia showed significant cytotoxic activity on A549 cells after 24 hours the extract a dose-dependent reduction in cell viability. IC50 values of above and below ground parts ethanolic extracts were determined as 7 µg/ml and 20 µg/ml respectively. Specifically, apoptosis inducing effect was increased at 7 and 20 µg/ml concentrations by 24 hours. We found that I. taochia ethanol extracts had antiproliferative and apoptotic effects on the human lung adenocarcinoma cells A549. However, further studies at molecular level are required to support our findings and to elucidate chemoproteventive and chemotherapeutic effects of I. taochia on lung cancer.

References

  • Asghar SF, Rehman HU, Rahman AU, Choudhary, MI. 2010. Phytochemical investigations on Iris germanica. Nat Prod Res, 24: 131-139.
  • Balunas MJ, Kinghorn AD. 2005. Drug discovery from medicinal plants. Life Sci, 78: 431-441.
  • Bayram E, Kırıcı E, Tansi S, Yılmaz G, Arabacı O, Kızıl S, Telci D. 2010. Possibilities of Increasing Production of Medical and Aromatic Plants. In: Turkey Agricultural Engineering Technical Conference Proceedings Book-I, January 11-15, Ankara, Türkiye, pp. 437-457.
  • Benetti L, Roizman B. 2007. In transduced cells, the US3 protein kinase of herpes simplex virus 1 recludes activation and induction of apoptosis by transfected procaspase 3. J Virol, 81: 10242-10248.
  • Bhalodia NR, Shukla VJ. 2011. Antibacterial and antifungal activities from leaf extracts of Cassia fistula l.: An ethnomedicinal plant. J Adv Pharm Technol Res, 2:104-109.
  • Bonfils JP, Pinguet F, Culine S, Sauvaire Y. 2001. Cytotoxicity of iridals, triterpenoids from Iris, on human tumor cell lines A2780 and K562. Planta Med, 67:79-81.
  • Bozyel ME, Bozyel EM, Canlı K, Altuner EM. 2019. Anticancer Uses of Medicinal Plants in Turkish Medicine. KSU J Agric Nat, 22: 465-484.
  • Conforti F, Menichini F, Rigano D, Senatore F. 2009. Antiproliferative activity on Human cancer cell lines after treatment with polyphenolic compounds isolated from Iris pseudopumila flowers and rhizomes. Z Naturforsch, 64:490-494.
  • Duan W, Zhang L. 2006. Cyclooxygenase inhibitors not inhibit resting lung cancer A549 cell proliferation. PLEFA, 74:317-321.
  • Edmondson JM, Armstrong LS, Martinez AO. 1988. A rapid and simple MTT-based spectrophotometric assay for determining drug sensitivity in monolayer cultures. Meth Cell Sci, 11:15-17.
  • Ekim T, Koyuncu M, Vural M, Duman H, Aytaç Z, Adıgüzel N. 2000. Endemics. In: Ekim T, Koyuncu M, Vural M, Duman H, Aytaç Z, Adıgüzel N, editors. Red Data of Turkish Plants, Nature Conservation Association Publications, Ankara, Turkey, pp 96.
  • Ertürk R, Çelik C, Kaygusuz R, Aydın H. 2010. Antimicrobial activities of commercial thyme and mint essential oils. CMJ, 32:281-286.
  • Fang R, Houghton PJ, Hylands PJ. 2008. Cytotoxic effects of compounds from Iris tectorum on human cancer cell lines. J Ethnopharmacol, 118:257-263.
  • Ghobrial IM, Witzig TE, Adjei AA. 2005. Targeting apoptosis pathways in cancer therapy. CA Cancer J Clin, 55:178-194.
  • Güner A. 2012. Iris L. In: Güner A, Aslan S, Ekim T, Vural M, Babaç MT, editors. Plants List of Turkey (Vascular Plants), Nezahat Gökyiğit Botanical Garden and Flora Research Association Publication İstanbul, Türkiye, pp 535-540.
  • Hacibekirglu I, Kolak U. 2011. Antioxidant and anticholinesterase constituents from the petroleum ether and chloroform extracts of Iris suaveolens. Phytother Res, 25: 522-529.
  • Hsieh CC, Hernández-Ledesma B, Ben O. 2010. Lunasin, a novel seed peptide, sensitizes human breast cancer MDA-MB-231 cells to aspirin-arrested cell cycle and induced apoptosis. Chem Biol Interact, 186: 127-134.
  • Huang KF, Zhang GD, Huang YQ, Diao, Y. 2012. Wogonin induces apoptosis and down-regulates survivin in human breast cancer MCF-7 cells by modulating PI3K-AKT pathway. Int. Immunopharmacol, 12:334-341.
  • Huwaitat S, Al-Khateeb E, Finjan S. 2013. Isolation and identification of some phytochemical compounds from different parts of I. nigricans. ESJ, 9:32-37.
  • Kandemir N. 2006. An investigation on the autecological of endemic Iris taochia Woronow ex Gross. (Iridaceae) distributed in the North East Anatolia Region. Pakistan J Biol Sci, 9:2753-2760.
  • Kandemir N, Yakupoğlu H. 2016. Morphological and anatomical properties of endemic Iris nezahatiae distributed in the North-East Anatolia region (Turkey). Bitlis Eren Univ J Sci Technol, 5:104-112.
  • Kandemir N, Kandemir Ş, Çelikoğlu E, Çelikoğlu U, İdil Ö. 2022. Antioxidant and antimicrobial activities of extracts obtained from different organs of Heliotropium myosotoides and Heliotropium suaveolens (Boraginaceae). BSJ Eng Sci, 5(3): https://doi.org/10.34248/bsengineering.1084135 (in press).
  • Kassak P. 2012. Secondary metabolites of the choosen genus Iris species. Acta Univ Agric et Silvic Mendel Brun, 60:269-280.
  • Kassak P. 2014. Screening of the chemical content of several Limniris group Irises. J Pharmacogn Phytochem, 3:11-14.
  • Kerr JF, Wyllie AH, Currie AR. 1972. Apoptosis: A basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer, 26:239-257.
  • Kukula-Koch W, Sieniawska E, Widelski J, Urjin O, Głowniak P, Skalicka-Woźniak K. 2015. Major secondary metabolites of Iris spp. Phytochem Rev, 14:51-80.
  • Ma Y, Li H, Lin B. 2012. C-glycosylflavones from the leaves of Iris tectorum Maxim. Acta Pharm Sin B, 2:598-601.
  • Majno G, Joris I. 1995. Apoptosis, oncosis, and necrosis. An overview of cell death. Am J Pathol, 146:3-15.
  • Mathew B. 1984. Iris L. In: Davis PH, Mill RR, Tan K, editors. Flora of Turkey and the East Aegean Islands. Edinburg University, Edinburg, 8th, pp. 382-410.
  • Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival:application to proliferation and cytotoxicity assays. J Ummunol Methods, 65: 55-63.
  • Nighat N, Surrinder K, Mushtaq AQ. 2008. Isoflovonos from I. kashmiriana. J Asian Nat Prod Res, 10: 1137-1141.
  • Obradovic A, Zizic J, Trisovic N, Bozic B, Uscumlic G, Bozic B, Markovic S. 2013. Evaluation of antioxidative effects of twelve 3-substituted-5, 5-diphenylhydantoins on human colon cancer cell line HCT-116. Turk J Biol. 37:741-747.
  • Orhan I, Nasim S, Tener B, Ayanoglu F, Özgüven M, Choudhary IM, Rahman AU. 2003. Two isoflavones and bioactivity spectrum of the crude extract if Iris germanica rhizomes. Phytother Res, 17: 575-577.
  • Özkan A, Erdogan A. 2012. Antioxidant and anticancer activity of fresh corm extract from Romulea tempskyana (Iridaceae). Nat Prod Res, 26:2126-2128.
  • Özkan A, Erdoğan A. 2013. Membrane and DNA damaging/protective effects of eugenol, eucalyptol, terpinen-4-ol, and camphor at various concentrations on parental and drug-resistant H1299 cells. Turk J Biol, 37:405-413.
  • Padmaharish V, Lakshmi T. 2017. Anticancer Activities of Medicinal Plants –An Update. J Pharm Sci Res, 9: 432-440.
  • Riedl SJ, Shi Y. 2004. Molecular mechanisms of caspase regulation during apoptosis. Nat Rev Mol Cell Biol, 5:897-907.
  • Rigano D, Conforti F, Formisano C, Menichini F, Senatore F. 2009. Comparative free radical scavenging potential and cytotoxicity of different extracts from of Iris pseudopumila Tineo flowers and rhizomes. Nat Prod Res, 23:17-25.
  • Rudin CM, Thompson CB. 1997. Apoptosis and disease: regulation and clinical relevance of programmed cell death. Ann Rev Med, 48:267-281.
  • Sabrin RMI, Gamal AM, Nawal MAM. 2012. New Constituents from the rhizomes of Egyptian Iris germanica L. Molecules, 17:2587-2598.
  • Spitz G A, Furtado CM, Sola-Penna M, Zancan P. 2009. Acetylsalicylic acid and salicylic acid decrease tumor cell viability and glucose metabolism modulating 6-phosphofructo-1-kinase structure and activity. Biochem Pharmacol, 77:46-53.
  • Tantry MA, Ghazanfar K, Zargar UR. 2013. New alkylated benzoquinone from Iris nepalensis. Nat Prod Res, 27:1832-1836.
  • Trusheva B, Trunkova D, Bankova V. 2007. Different extraction methods of biologically active components from propolis: a preliminary study. Chem Cent J, 1:1-13.
  • Vejselova D, Kutlu HM. 2015. Inhibitory effects of salicylic acid on A549 human lung adenocarcinoma cell viability. Turk J Biol 39: 1-5.
  • Wang YQ, Tan JJ, Tan CH, Jiang SH, Zhu DY. 2003. Halophilols A and B, two new stilbenes from Iris halophila. Planta Med, 69:779-781.
  • Wollenweber E, Stevens JF, Klimo K, Kanauft J, Norbert F, Clarissa G. 2003. Cancer chemopreventive in vitro activities of isoflavones isolated from Iris germanica. Planta Med, 69:15-20.
  • Wyllie AH, Kerr JR, Currie AR. 1980. Cell death: the significance of apoptosis. Int Rev Cytol, 68:251-306.
  • Wyllie AH. 1993. Apoptosis (the 1992 Frank Rose memorial lecture). Br J Cancer, 67:205-208.
  • Xie X, Tian Y, Yin S, Lin Y, Tan G. 2013. Anticancer effects of Ligusticum chuanxiong Hort alcohol extracts on HS766 T cell. Afr J Tradit Complement Altern Med, 10:542-546.
  • Yazgan B, Özçelik Ö, Ayar A, Renda G, Yıldırım T. 2022. Cytotoxic and Apoptotic Effect of Iris taochia Plant Extracts on Human Breast Cancer (MCF-7) Cell. Curr Proteom, 19:91-101.

Year 2022, Volume 5, Issue 3, 395 - 403, 01.09.2022
https://doi.org/10.19127/bshealthscience.1100668

Abstract

References

  • Asghar SF, Rehman HU, Rahman AU, Choudhary, MI. 2010. Phytochemical investigations on Iris germanica. Nat Prod Res, 24: 131-139.
  • Balunas MJ, Kinghorn AD. 2005. Drug discovery from medicinal plants. Life Sci, 78: 431-441.
  • Bayram E, Kırıcı E, Tansi S, Yılmaz G, Arabacı O, Kızıl S, Telci D. 2010. Possibilities of Increasing Production of Medical and Aromatic Plants. In: Turkey Agricultural Engineering Technical Conference Proceedings Book-I, January 11-15, Ankara, Türkiye, pp. 437-457.
  • Benetti L, Roizman B. 2007. In transduced cells, the US3 protein kinase of herpes simplex virus 1 recludes activation and induction of apoptosis by transfected procaspase 3. J Virol, 81: 10242-10248.
  • Bhalodia NR, Shukla VJ. 2011. Antibacterial and antifungal activities from leaf extracts of Cassia fistula l.: An ethnomedicinal plant. J Adv Pharm Technol Res, 2:104-109.
  • Bonfils JP, Pinguet F, Culine S, Sauvaire Y. 2001. Cytotoxicity of iridals, triterpenoids from Iris, on human tumor cell lines A2780 and K562. Planta Med, 67:79-81.
  • Bozyel ME, Bozyel EM, Canlı K, Altuner EM. 2019. Anticancer Uses of Medicinal Plants in Turkish Medicine. KSU J Agric Nat, 22: 465-484.
  • Conforti F, Menichini F, Rigano D, Senatore F. 2009. Antiproliferative activity on Human cancer cell lines after treatment with polyphenolic compounds isolated from Iris pseudopumila flowers and rhizomes. Z Naturforsch, 64:490-494.
  • Duan W, Zhang L. 2006. Cyclooxygenase inhibitors not inhibit resting lung cancer A549 cell proliferation. PLEFA, 74:317-321.
  • Edmondson JM, Armstrong LS, Martinez AO. 1988. A rapid and simple MTT-based spectrophotometric assay for determining drug sensitivity in monolayer cultures. Meth Cell Sci, 11:15-17.
  • Ekim T, Koyuncu M, Vural M, Duman H, Aytaç Z, Adıgüzel N. 2000. Endemics. In: Ekim T, Koyuncu M, Vural M, Duman H, Aytaç Z, Adıgüzel N, editors. Red Data of Turkish Plants, Nature Conservation Association Publications, Ankara, Turkey, pp 96.
  • Ertürk R, Çelik C, Kaygusuz R, Aydın H. 2010. Antimicrobial activities of commercial thyme and mint essential oils. CMJ, 32:281-286.
  • Fang R, Houghton PJ, Hylands PJ. 2008. Cytotoxic effects of compounds from Iris tectorum on human cancer cell lines. J Ethnopharmacol, 118:257-263.
  • Ghobrial IM, Witzig TE, Adjei AA. 2005. Targeting apoptosis pathways in cancer therapy. CA Cancer J Clin, 55:178-194.
  • Güner A. 2012. Iris L. In: Güner A, Aslan S, Ekim T, Vural M, Babaç MT, editors. Plants List of Turkey (Vascular Plants), Nezahat Gökyiğit Botanical Garden and Flora Research Association Publication İstanbul, Türkiye, pp 535-540.
  • Hacibekirglu I, Kolak U. 2011. Antioxidant and anticholinesterase constituents from the petroleum ether and chloroform extracts of Iris suaveolens. Phytother Res, 25: 522-529.
  • Hsieh CC, Hernández-Ledesma B, Ben O. 2010. Lunasin, a novel seed peptide, sensitizes human breast cancer MDA-MB-231 cells to aspirin-arrested cell cycle and induced apoptosis. Chem Biol Interact, 186: 127-134.
  • Huang KF, Zhang GD, Huang YQ, Diao, Y. 2012. Wogonin induces apoptosis and down-regulates survivin in human breast cancer MCF-7 cells by modulating PI3K-AKT pathway. Int. Immunopharmacol, 12:334-341.
  • Huwaitat S, Al-Khateeb E, Finjan S. 2013. Isolation and identification of some phytochemical compounds from different parts of I. nigricans. ESJ, 9:32-37.
  • Kandemir N. 2006. An investigation on the autecological of endemic Iris taochia Woronow ex Gross. (Iridaceae) distributed in the North East Anatolia Region. Pakistan J Biol Sci, 9:2753-2760.
  • Kandemir N, Yakupoğlu H. 2016. Morphological and anatomical properties of endemic Iris nezahatiae distributed in the North-East Anatolia region (Turkey). Bitlis Eren Univ J Sci Technol, 5:104-112.
  • Kandemir N, Kandemir Ş, Çelikoğlu E, Çelikoğlu U, İdil Ö. 2022. Antioxidant and antimicrobial activities of extracts obtained from different organs of Heliotropium myosotoides and Heliotropium suaveolens (Boraginaceae). BSJ Eng Sci, 5(3): https://doi.org/10.34248/bsengineering.1084135 (in press).
  • Kassak P. 2012. Secondary metabolites of the choosen genus Iris species. Acta Univ Agric et Silvic Mendel Brun, 60:269-280.
  • Kassak P. 2014. Screening of the chemical content of several Limniris group Irises. J Pharmacogn Phytochem, 3:11-14.
  • Kerr JF, Wyllie AH, Currie AR. 1972. Apoptosis: A basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer, 26:239-257.
  • Kukula-Koch W, Sieniawska E, Widelski J, Urjin O, Głowniak P, Skalicka-Woźniak K. 2015. Major secondary metabolites of Iris spp. Phytochem Rev, 14:51-80.
  • Ma Y, Li H, Lin B. 2012. C-glycosylflavones from the leaves of Iris tectorum Maxim. Acta Pharm Sin B, 2:598-601.
  • Majno G, Joris I. 1995. Apoptosis, oncosis, and necrosis. An overview of cell death. Am J Pathol, 146:3-15.
  • Mathew B. 1984. Iris L. In: Davis PH, Mill RR, Tan K, editors. Flora of Turkey and the East Aegean Islands. Edinburg University, Edinburg, 8th, pp. 382-410.
  • Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival:application to proliferation and cytotoxicity assays. J Ummunol Methods, 65: 55-63.
  • Nighat N, Surrinder K, Mushtaq AQ. 2008. Isoflovonos from I. kashmiriana. J Asian Nat Prod Res, 10: 1137-1141.
  • Obradovic A, Zizic J, Trisovic N, Bozic B, Uscumlic G, Bozic B, Markovic S. 2013. Evaluation of antioxidative effects of twelve 3-substituted-5, 5-diphenylhydantoins on human colon cancer cell line HCT-116. Turk J Biol. 37:741-747.
  • Orhan I, Nasim S, Tener B, Ayanoglu F, Özgüven M, Choudhary IM, Rahman AU. 2003. Two isoflavones and bioactivity spectrum of the crude extract if Iris germanica rhizomes. Phytother Res, 17: 575-577.
  • Özkan A, Erdogan A. 2012. Antioxidant and anticancer activity of fresh corm extract from Romulea tempskyana (Iridaceae). Nat Prod Res, 26:2126-2128.
  • Özkan A, Erdoğan A. 2013. Membrane and DNA damaging/protective effects of eugenol, eucalyptol, terpinen-4-ol, and camphor at various concentrations on parental and drug-resistant H1299 cells. Turk J Biol, 37:405-413.
  • Padmaharish V, Lakshmi T. 2017. Anticancer Activities of Medicinal Plants –An Update. J Pharm Sci Res, 9: 432-440.
  • Riedl SJ, Shi Y. 2004. Molecular mechanisms of caspase regulation during apoptosis. Nat Rev Mol Cell Biol, 5:897-907.
  • Rigano D, Conforti F, Formisano C, Menichini F, Senatore F. 2009. Comparative free radical scavenging potential and cytotoxicity of different extracts from of Iris pseudopumila Tineo flowers and rhizomes. Nat Prod Res, 23:17-25.
  • Rudin CM, Thompson CB. 1997. Apoptosis and disease: regulation and clinical relevance of programmed cell death. Ann Rev Med, 48:267-281.
  • Sabrin RMI, Gamal AM, Nawal MAM. 2012. New Constituents from the rhizomes of Egyptian Iris germanica L. Molecules, 17:2587-2598.
  • Spitz G A, Furtado CM, Sola-Penna M, Zancan P. 2009. Acetylsalicylic acid and salicylic acid decrease tumor cell viability and glucose metabolism modulating 6-phosphofructo-1-kinase structure and activity. Biochem Pharmacol, 77:46-53.
  • Tantry MA, Ghazanfar K, Zargar UR. 2013. New alkylated benzoquinone from Iris nepalensis. Nat Prod Res, 27:1832-1836.
  • Trusheva B, Trunkova D, Bankova V. 2007. Different extraction methods of biologically active components from propolis: a preliminary study. Chem Cent J, 1:1-13.
  • Vejselova D, Kutlu HM. 2015. Inhibitory effects of salicylic acid on A549 human lung adenocarcinoma cell viability. Turk J Biol 39: 1-5.
  • Wang YQ, Tan JJ, Tan CH, Jiang SH, Zhu DY. 2003. Halophilols A and B, two new stilbenes from Iris halophila. Planta Med, 69:779-781.
  • Wollenweber E, Stevens JF, Klimo K, Kanauft J, Norbert F, Clarissa G. 2003. Cancer chemopreventive in vitro activities of isoflavones isolated from Iris germanica. Planta Med, 69:15-20.
  • Wyllie AH, Kerr JR, Currie AR. 1980. Cell death: the significance of apoptosis. Int Rev Cytol, 68:251-306.
  • Wyllie AH. 1993. Apoptosis (the 1992 Frank Rose memorial lecture). Br J Cancer, 67:205-208.
  • Xie X, Tian Y, Yin S, Lin Y, Tan G. 2013. Anticancer effects of Ligusticum chuanxiong Hort alcohol extracts on HS766 T cell. Afr J Tradit Complement Altern Med, 10:542-546.
  • Yazgan B, Özçelik Ö, Ayar A, Renda G, Yıldırım T. 2022. Cytotoxic and Apoptotic Effect of Iris taochia Plant Extracts on Human Breast Cancer (MCF-7) Cell. Curr Proteom, 19:91-101.

Details

Primary Language English
Subjects Medicine
Journal Section Research Article
Authors

Nezahat KANDEMİR> (Primary Author)
AMASYA ÜNİVERSİTESİ
0000-0002-5428-4139
Türkiye


Emine ÇELİKOĞLU>
AMASYA ÜNİVERSİTESİ
0000-0002-5956-0008
Türkiye


Şevket KANDEMİR>
AMASYA ÜNİVERSİTESİ
0000-0001-6781-0057
Türkiye


Umut ÇELİKOĞLU>
AMASYA ÜNİVERSİTESİ
0000-0003-0995-8154
Türkiye


Önder İDİL>
AMASYA ÜNİVERSİTESİ
0000-0003-1744-4006
Türkiye


Canan VEJSELOVA SEZER>
ESKİŞEHİR TEKNİK ÜNİVERSİTESİ
0000-0002-3792-5993
Türkiye


Hatice Mehtap KUTLU>
ESKİŞEHİR TEKNİK ÜNİVERSİTESİ
0000-0002-8816-1487
Türkiye

Supporting Institution Bu makale çalışmasını destekleyen bir kurum bulunmamaktadır
Project Number Bir proje çalışması değildir.
Publication Date September 1, 2022
Application Date April 8, 2022
Acceptance Date April 28, 2022
Published in Issue Year 2022, Volume 5, Issue 3

Cite

Bibtex @research article { bshealthscience1100668, journal = {Black Sea Journal of Health Science}, eissn = {2619-9041}, address = {bsjhealthsci@blackseapublishers.com}, publisher = {Cem TIRINK}, year = {2022}, volume = {5}, number = {3}, pages = {395 - 403}, doi = {10.19127/bshealthscience.1100668}, title = {The Impact of Endemic Iris taochia Ethanolic Extracts on Human Lung Adenocarcinoma Cells}, key = {cite}, author = {Kandemir, Nezahat and Çelikoğlu, Emine and Kandemir, Şevket and Çelikoğlu, Umut and İdil, Önder and Vejselova Sezer, Canan and Kutlu, Hatice Mehtap} }
APA Kandemir, N. , Çelikoğlu, E. , Kandemir, Ş. , Çelikoğlu, U. , İdil, Ö. , Vejselova Sezer, C. & Kutlu, H. M. (2022). The Impact of Endemic Iris taochia Ethanolic Extracts on Human Lung Adenocarcinoma Cells . Black Sea Journal of Health Science , 5 (3) , 395-403 . DOI: 10.19127/bshealthscience.1100668
MLA Kandemir, N. , Çelikoğlu, E. , Kandemir, Ş. , Çelikoğlu, U. , İdil, Ö. , Vejselova Sezer, C. , Kutlu, H. M. "The Impact of Endemic Iris taochia Ethanolic Extracts on Human Lung Adenocarcinoma Cells" . Black Sea Journal of Health Science 5 (2022 ): 395-403 <https://dergipark.org.tr/en/pub/bshealthscience/issue/68048/1100668>
Chicago Kandemir, N. , Çelikoğlu, E. , Kandemir, Ş. , Çelikoğlu, U. , İdil, Ö. , Vejselova Sezer, C. , Kutlu, H. M. "The Impact of Endemic Iris taochia Ethanolic Extracts on Human Lung Adenocarcinoma Cells". Black Sea Journal of Health Science 5 (2022 ): 395-403
RIS TY - JOUR T1 - The Impact of Endemic Iris taochia Ethanolic Extracts on Human Lung Adenocarcinoma Cells AU - NezahatKandemir, EmineÇelikoğlu, ŞevketKandemir, UmutÇelikoğlu, Önderİdil, CananVejselova Sezer, Hatice MehtapKutlu Y1 - 2022 PY - 2022 N1 - doi: 10.19127/bshealthscience.1100668 DO - 10.19127/bshealthscience.1100668 T2 - Black Sea Journal of Health Science JF - Journal JO - JOR SP - 395 EP - 403 VL - 5 IS - 3 SN - -2619-9041 M3 - doi: 10.19127/bshealthscience.1100668 UR - https://doi.org/10.19127/bshealthscience.1100668 Y2 - 2022 ER -
EndNote %0 Black Sea Journal of Health Science The Impact of Endemic Iris taochia Ethanolic Extracts on Human Lung Adenocarcinoma Cells %A Nezahat Kandemir , Emine Çelikoğlu , Şevket Kandemir , Umut Çelikoğlu , Önder İdil , Canan Vejselova Sezer , Hatice Mehtap Kutlu %T The Impact of Endemic Iris taochia Ethanolic Extracts on Human Lung Adenocarcinoma Cells %D 2022 %J Black Sea Journal of Health Science %P -2619-9041 %V 5 %N 3 %R doi: 10.19127/bshealthscience.1100668 %U 10.19127/bshealthscience.1100668
ISNAD Kandemir, Nezahat , Çelikoğlu, Emine , Kandemir, Şevket , Çelikoğlu, Umut , İdil, Önder , Vejselova Sezer, Canan , Kutlu, Hatice Mehtap . "The Impact of Endemic Iris taochia Ethanolic Extracts on Human Lung Adenocarcinoma Cells". Black Sea Journal of Health Science 5 / 3 (September 2022): 395-403 . https://doi.org/10.19127/bshealthscience.1100668
AMA Kandemir N. , Çelikoğlu E. , Kandemir Ş. , Çelikoğlu U. , İdil Ö. , Vejselova Sezer C. , Kutlu H. M. The Impact of Endemic Iris taochia Ethanolic Extracts on Human Lung Adenocarcinoma Cells. BSJ Health Sci.. 2022; 5(3): 395-403.
Vancouver Kandemir N. , Çelikoğlu E. , Kandemir Ş. , Çelikoğlu U. , İdil Ö. , Vejselova Sezer C. , Kutlu H. M. The Impact of Endemic Iris taochia Ethanolic Extracts on Human Lung Adenocarcinoma Cells. Black Sea Journal of Health Science. 2022; 5(3): 395-403.
IEEE N. Kandemir , E. Çelikoğlu , Ş. Kandemir , U. Çelikoğlu , Ö. İdil , C. Vejselova Sezer and H. M. Kutlu , "The Impact of Endemic Iris taochia Ethanolic Extracts on Human Lung Adenocarcinoma Cells", Black Sea Journal of Health Science, vol. 5, no. 3, pp. 395-403, Sep. 2022, doi:10.19127/bshealthscience.1100668