Antibiofilm, Antioxidant and Quorum Quenching Activities of Propolis Samples from Southwest Anatolia

Abstract

In this study, antibiofilm, antioxidant and quorum quenching activities of the ethanol extracts of propolis samples collected from Muğla district were investigated. Antimicrobial activity was determined using the well diffusion and broth tube dilution methods, antibiofilm activity with microplate biofilm method, and antioxidant activity with DPPH radical scavenging, β-carotene linoleic acid and ferric thiocyanate methods. To determine the antimicrobial activity of the extracts, Listeria monocytogenes ATCC 7944, Streptococcus mutans CNCTC 8/77, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Salmonella typhimurium ATCC 14028 and Candida albicans ATCC 10239 strains were used. Minimum inhibitory concentrations (MIC) against microorganisms were determined from 1 to >100 mg/ml. The lowest MIC value was found as 1 mg/ml for AP6 propolis extract against Salmonella typhimurium. According to the antibiofilm activity results, highest biofilm were detected at concentrations of MIC as 82.60% for AP1 against S. mutans, 67.45% for AP2 against L. monocytogenes, 73.02% for AP3 against S. mutans, 64.05% for AP4 against L. monocytogenes, 70.58% for AP5 against S. typhimurium; 93.43% for AP6 against S. typhimurium and 72.43% for AP7 against S. mutans. AP7 extract had the highest antioxidant activity with an IC50 value of 3.94 mg/ml for DPPH radical scavenging method and with 91.10% reduction rate for β-carotene linoleic acid method. AP1 extract had the highest reduction percentage rate of 51.77% in the ferric thiocyanate method.

Keywords

• Antimicrobial activity
• Minimum Inhibition Concentration
• Anti-QS
• Mugla

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