Antibiofilm, Antioxidant and Quorum Quenching Activities of Propolis Samples from Southwest Anatolia

Year 2020, Volume: 12 Issue: 2, 49 - 55, 31.12.2020

Özgür Ceylan Halime Alıç

https://doi.org/10.51458/BSTD.2021.9

Abstract

In this study, antibiofilm, antioxidant and quorum quenching activities of the ethanol extracts of propolis samples collected from Muğla district were investigated. Antimicrobial activity was determined using the well diffusion and broth tube dilution methods, antibiofilm activity with microplate biofilm method, and antioxidant activity with DPPH radical scavenging, β-carotene linoleic acid and ferric thiocyanate methods. To determine the antimicrobial activity of the extracts, Listeria monocytogenes ATCC 7944, Streptococcus mutans CNCTC 8/77, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Salmonella typhimurium ATCC 14028 and Candida albicans ATCC 10239 strains were used. Minimum inhibitory concentrations (MIC) against microorganisms were determined from 1 to >100 mg/ml. The lowest MIC value was found as 1 mg/ml for AP6 propolis extract against Salmonella typhimurium. According to the antibiofilm activity results, highest biofilm were detected at concentrations of MIC as 82.60% for AP1 against S. mutans, 67.45% for AP2 against L. monocytogenes, 73.02% for AP3 against S. mutans, 64.05% for AP4 against L. monocytogenes, 70.58% for AP5 against S. typhimurium; 93.43% for AP6 against S. typhimurium and 72.43% for AP7 against S. mutans. AP7 extract had the highest antioxidant activity with an IC50 value of 3.94 mg/ml for DPPH radical scavenging method and with 91.10% reduction rate for β-carotene linoleic acid method. AP1 extract had the highest reduction percentage rate of 51.77% in the ferric thiocyanate method.

Keywords

Antimicrobial activity, Minimum Inhibition Concentration, Anti-QS, Mugla

Supporting Institution

Muğla Sıtkı Koçman Üniversitesi BAP Projesi

Project Number

BAP 13/187

Thanks

This study is a part of MSc thesis and supported by Muğla Sıtkı Koçman University, with BAP 13/178 project number.

References

• Alencar, S. M., Oldoni, T. C., Castro, M. L., Cabral, I. S. R., Costa-Neto, C. M., Cury, J. A., ... & Ikegaki, M. (2007). Chemical composition and biological activity of a new type of Brazilian propolis: red propolis. Journal of ethnopharmacology, 113(2), 278-283.
• Anjum, S. I., Ullah, A., Khan, K. A., Attaullah, M., Khan, H., Ali, H., ... & Adgaba, N. (2019). Composition and functional properties of propolis (bee glue): A review. Saudi Journal of Biological Sciences, 26(7), 1695-1703.
• Bankova, V. S., de Castro, S. L., & Marcucci, M. C. (2000). Propolis: recent advances in chemistry and plant origin. Apidologie, 31(1), 3-15.
• Blicharska, N., & Seidel, V. (2019). Chemical diversity and biological activity of african propolis. In Progress in the Chemistry of Organic Natural Products 109 (pp. 415-450). Springer, Cham.
• Blonska, M., Bronikowska, J., Pietsz, G., Czuba, Z. P., Scheller, S., & Krol, W. (2004). Effects of ethanol extract of propolis (EEP) and its flavones on inducible gene expression in J774A. 1 macrophages. Journal of Ethnopharmacology, 91(1), 25-30.
• Burits, M., & Bucar, F. (2000). Antioxidant activity of Nigella sativa essential oil. Phytotherapy research, 14(5), 323-328.
• Capoci, I. R. G., Bonfim-Mendonça, P. D. S., Arita, G. S., Pereira, R. R. D. A., Consolaro, M. E. L., Bruschi, M. L., ... & Svidzinski, T. I. E. (2015). Propolis is an efficient fungicide and inhibitor of biofilm production by vaginal Candida albicans. Evidence-Based Complementary and Alternative Medicine, 2015.
• Chen, C.N. , Weng, M.S. , Wu, C.L. ve Lin, J.K. (2004) Comparison of Radical Scavenging Activity, Cytotoxic Effects and Apoptosis Induction in Human Melanoma Cells by Taiwanese Propolis from Different Sources, Evidence Based Complementary and Alternative Medicine, 1(2):175-185.
• Cheng, P. C., & Wong, G. (1996). Honey bee propolis: prospects in medicine. Bee world, 77(1), 8-15.
• Choi, Y. M., Noh, D. O., Cho, S. Y., Suh, H. J., Kim, K. M., & Kim, J. M. (2006). Antioxidant and antimicrobial activities of propolis from several regions of Korea. LWT-Food Science and Technology, 39(7), 756-761.
• Choo, J. H., Rukayadi, Y., & Hwang, J. K. (2006). Inhibition of bacterial quorum sensing by vanilla extract. Letters in applied microbiology, 42(6), 637-641.
• Dapkevicius, A., Venskutonis, R., van Beek, T. A., & Linssen, J. P. (1998). Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in Lithuania. Journal of the Science of Food and Agriculture, 77(1), 140-146.
• Dogan, N., Doğanlı, G.,Ulger, G.,Habesoglu, D., Guzel, S., Yasar, Y., Arar, D.,Sensoy, T. and Bozbeyoglu, N. (2014). Antibiofilm effect of two propolis samples from Turkey. Journal of Applied Biological Sciences, 8(2), 27-31.
• Ghisalberti, E. L. (1979). Propolis: a review. Bee world, 60(2), 59-84.
• Gonçalves, G. M. S., Santos, N. P., & Srebernich, S. M. (2011). Antioxidant and antimicrobial activities of propolis and açai (Euterpe oleracea Mart) extracts. Journal of Basic and Applied Pharmaceutical Sciences, 32(3).
• Koh, K. H., & Tham, F. Y. (2011). Screening of traditional Chinese medicinal plants for quorum-sensing inhibitors activity. Journal of Microbiology, Immunology and Infection, 44(2), 144-148.
• Kouidhi, B., Zmantar, T., & Bakhrouf, A. (2010). Anti-cariogenic and anti-biofilms activity of Tunisian propolis extract and its potential protective effect against cancer cells proliferation. Anaerobe, 16(6), 566-571.
• Lu, L. C., Chen, Y. W., & Chou, C. C. (2003). Antibacterial and DPPH free radical-scavenging activities of the ethanol extract of propolis collected in Taiwan. Journal of Food and Drug Analysis, 11(4), 277-282.
• Marcucci, M. C. (1995). Propolis: chemical composition, biological properties and therapeutic activity.
• Merritt, J. H., & Kadouri, D. E. O, Toole, GA (2005) Growing and analyzing static biofilms. Current Protocols in Microbiology, 1.
• Miguel, M. G., Nunes, S., Dandlen, S. A., Cavaco, A. M., & Antunes, M. D. (2010). Phenols and antioxidant activity of hydro-alcoholic extracts of propolis from Algarve, South of Portugal. Food and Chemical Toxicology, 48(12), 3418-3423.
• Mitsuda, H., Yuasumoto, K., Iwami, K. (1996) Antioxidation action of indole compounds during the autoxidation of linoleic acid, Journal of Japanese Society of Nutrition and Food Science, 19:210.
• Moreira, L., Dias, L. G., Pereira, J. A., & Estevinho, L. (2008). Antioxidant properties, total phenols and pollen analysis of propolis samples from Portugal. Food and Chemical toxicology, 46(11), 3482-3485.
• Moreno, M. I. N., Isla, M. I., Sampietro, A. R., & Vattuone, M. A. (2000). Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. Journal of ethnopharmacology, 71(1-2), 109-114.
• Russo, A., Cardile, V., Sanchez, F., Troncoso, N., Vanella, A., & Garbarino, J. A. (2004). Chilean propolis: antioxidant activity and antiproliferative action in human tumor cell lines. Life sciences, 76(5), 545-558.
• Piccinelli, A. L., Mencherini, T., Celano, R., Mouhoubi, Z., Tamendjari, A., Aquino, R. P., & Rastrelli, L. (2013). Chemical composition and antioxidant activity of Algerian propolis. Journal of agricultural and food chemistry, 61(21), 5080-5088.
• Rojczyk, E., Klama-Baryła, A., Łabuś, W., Wilemska-Kucharzewska, K., & Kucharzewski, M. (2020). Historical and modern research on propolis and its application in wound healing and other fields of medicine and contributions by Polish studies. Journal of Ethnopharmacology, 113159.
• Savka, M. A., Dailey, L., Popova, M., Mihaylova, R., Merritt, B., Masek, M., ... & Bankova, V. (2015). Chemical composition and disruption of quorum sensing signaling in geographically diverse United States propolis. Evidence-Based Complementary and Alternative Medicine, 2015.
• Scazzocchio, F., D’auria, F. D., Alessandrini, D., & Pantanella, F. (2006). Multifactorial aspects of antimicrobial activity of propolis. Microbiological research, 161(4), 327-333.
• Sheng, J., Zhou, J., Wang, L., Xu, J., & Hu, Q. (2007). Antioxidant activity of ethanol and petroleum ether extracts from Brazilian propolis. European Food Research and Technology, 225(2), 249.
• Silva Frozza, C. O., Garcia, C. S. C., Gambato, G., de Souza, M. D. O., Salvador, M., Moura, S., ... & Dellagostin, O. A. (2013). Chemical characterization, antioxidant and cytotoxic activities of Brazilian red propolis. Food and chemical toxicology, 52, 137-142.
• Talla, E., Tamfu, A. N., Gade, I. S., Yanda, L., Mbafor, J. T., Laurent, S., ... & Bankova, V. (2017). New mono-ether of glycerol and triterpenes with DPPH radical scavenging activity from Cameroonian propolis. Natural product research, 31(12), 1379-1389.
• Tamfu, A. N., Sawalda, M., Fotsing, M. T., Kouipou, R. M. T., Talla, E., Chi, G. F., ... & Shaheen, F. (2020). A new isoflavonol and other constituents from Cameroonian propolis and evaluation of their anti-inflammatory, antifungal and antioxidant potential. Saudi Journal of Biological Sciences, 27(6), 1659-1666.