Surveying, identification and characterization for the potential honeybee (Apis mellifera L.) pollen sources in the arid region of Riyadh-Saudi Arabia
Year 2023,
Volume: 15 Issue: 1, 1 - 11, 31.07.2023
Abdulraouf Amro
,
Mohamed Omar Mohamed
Ahmad Al Ghamdi
Abstract
This study was conducted targeting identification and documentation of major
honeybee plants as pollen sources and their phenology in the central arid region
of Saudi Arabia (Riyadh). Pollen loads were collected using pollen traps and were
classified according to their color then traced back to plant species level.
Throughout the year, sixteen plant species belonging to 10 plant families has been
recorded and investigated by Light Microscopy (LM) and Scanning Electron
Microscopy (SEM). Asian mustard (Brassica tournefortii Gouan.) (15.91%), willow
wattle (Acacia salicina Lindl.) (15.17%), mesquite (Prosopis juliflora) (Sw.) DC.
(21.81%) and cat's head (Tribulus terresetris L. var. terrestris) (15.52%) were the
dominant sources of pollen. The period from May to July was found to be a
significant blooming period and the scarcity period was from December to March
in the study area. Color, size and shape of the potential pollen sources were
variable between different species. Beekeepers could trap pollen loads that were
collected from these sources during February, April and July, respectively. In
conclusion, wise use and rehabilitation of these potentially identified bee plant
species shall be considered in attempting beekeeping development.
Thanks
The authors would like to thank the curators of the
National Herbarium (RIY) in the Botany and
Microbiology department for providing herbarium
specimens. Thanks are also extended to the staff at the
SEM, Structural Studies Research Service for providing
the facilities.
References
- Abd El Salam, E. H., Ali, M. A., & Ghazala, N. A. (2022).
Evaluation of the Efficiency Different Types of Bee Pollen
Collection Traps in Honey Bee Colonies during Summer
Season. Arab Universities Journal of Agricultural
Sciences, 30(1), 141-146.
http://10.21608/AJS.2022.111909.1448.
- Abou-Shaara, H. F. (2015). Potential honey bee plants of Egypt.
Cerceta˘ri Agronomice în Moldova, 48, 99–108.
https://repository.uaiasi.ro/xmlui/handle/20.500.12811
/1530.
- Abou-Shaara, H. F., Ahmad A. Al-Ghamdi, & Abdelsalam, A. M.
(2013). A suitability map for keeping honey bees under
harsh environmental conditions using Geographical
Information System. World Applied Sciences Journal, 22, 81099-1105. https://doi.org/
10.5829/idosi.wasj.2013.22.08.7384.
- Adgaba, N., Alghamdi, A., Sammoud, R., Shenkute, A., Tadesse,
Y., Ansari, M. J., Sharma, D., & Hepburn, C. (2017).
Determining spatio-temporal distribution of bee forage
species of Al-Baha region based on ground inventorying
supported with GIS applications and Remote Sensed
Satellite Image analysis. Saudi journal of biological
sciences, 24, 1048–1055.
https://doi.org/10.1016/j.sjbs.2017.01.009.
- Agashe, S. N. (2021). Aerobiology for the Clinician: Basic and
Applied Aspects, Pollen Sources, Pollen Calendars. In
Textbook of Allergy for the Clinician, CRC Press, 49-68.
- Al-Ghamdi, A. A. (2007a). Evaluation of various honey bee
foraging activities for identification of potential bee
plants in Riyadh, Saudi Arabia. Annals of Agricultural
Science-Cairo, 52(2), 487.
- Al-Ghamdi, A. A. (2007b). Beekeeping and honey production in
Saudi Arabia. Fifth Conference of Arab Beekeepers
Association. Tripoli, Libya.
- Al-Ghamdi, A. (2009). Comprehensive study for the current
beekeeping industry of imported and native honey bee
in Kingdom of Saudi Arabia. King Abdulaziz City for
Science and Technology, Final report of project, AT-21-
39.
- Al-Ghamdi, A. A., Tadesse, Y., & Adgaba, N. (2020). Evaluation
of major Acacia species in the nursery towards
apicultural landscape restoration around Southwestern
Saudi Arabia. Saudi Journal of Biological Sciences, 27(12),
3385-3389. https://doi.org/10.1016/j.sjbs.2020.09.002.
- Al-Jabr, A. M., & Nour, M. E. (2001). Content of some Saudi
honeys of pollen spectrum and sucrose. Journal of
Agriculture Science, Mansoura University, 26: 4009-
4014.
- Alqarni, A. S. (2020). Differential foraging of indigenous and
exotic honey bee (Apis mellifera L.) races on nectar-rich
flow in a subtropical ecosystem. Insects, 11(4), 254.
https://doi.org/10.3390/insects11040254.
- Alves, R. De França, & Dos Santos De Assis R. F. (2016).
Arecaceae potential for production of monofloral bee
pollen. Grana, 56(4), 294-303.
https://doi.org/10.1080/00173134.2016.1239760.
- Amro, A. M. (2021). Pollinators and pollination effects on three
canola (Brassica napus L.) cultivars: A case study in
Upper Egypt. Journal of King Saud University-Science,
33(1), 101240.
https://doi.org/10.1016/j.jksus.2020.101240.
- Amro, A. M., Omar, M. O., & Ahmed, A. G. (2020). Impact of
using pollen substitutes on performance of honey bee
(Apis mellifera L.) colonies under harsh environmental
conditions. Science and Animal Health, 8(3), 236-256.
https://doi.org/10.15210/sah.v8i3.19992.
- Arien, Y., Dag, A., Yona, S., Tietel, Z., Cohen, T. L., & Shafir, S.
(2020). Effect of diet lipids and omega-6: 3 ratio on
honey bee brood development, adult survival and body
composition. Journal of insect physiology, 124, 104074.
https://doi.org/10.1016/j.jinsphys.2020.104074.
- Asefa, M., Cao, M., He, Y., Mekonnen, E., Song, X., & Yang, J.
(2020). Ethiopian vegetation types, climate and
topography. Plant Diversity, 42(4), 302-311.
https://doi.org/10.1016/j.pld.2020.04.004.
- Begum, H. A., Iqbal, J., & Aziz, A. (2021). Characterization of
pollen profile of Apis mellifera L. in arid region of
Pakistan. Saudi Journal of Biological Sciences, 28(5),
2964-2974. https://doi.org/10.1016/j.sjbs.2021.02.035.
- Belay, A., Solomon, W. K., Bultossa, G., Adgaba, N., & Melaku,
S. (2015). Botanical origin, colour, granulation, and
sensory properties of the Harenna forest honey, Bale,
Ethiopia. Food chemistery, 167, 213-219.
http://dx.doi.org/10.1016/j.foodchem.2014.06.080
- Bilisik, A., Cakmak, I., Saatcioglu, G., Bicakci, A., & Malyer, H.
(2008). Spectrum of pollen collected by honey bees in
bursa lowland area in high season. Uludag Bee Journal,
8(4), 143-148.
https://dergipark.org.tr/en/pub/uluaricilik/issue/53267
/162401
- Boesewinkel, F. D., & Bouman, F. (1984). The Seed: Structure.
In Embryology of Angiosperms, 1ed. Johri, Springer
Berlin Heidelberg, B.,567-610.
- Brodschneider,R.,&Crailsheim,K.(2010). Nutritionandhealthin
honey bees. Apidologie, 41(3), 278-294.
- Collenette, S. (1999). Wild Flowers of Saudi Arabia. National
Commission for Wild Life Conservation and
Development (VCWCD), Riyadh, Saudi Arabia, 799.
- Da luz, C. F. P., Junior, G. L. B., Fonseca, R. L. S. E., & DE Sousa,
P. R. (2010). Comparative pollen preferences by
Africanized honey bees Apis mellifera L. of two colonies
in Pará de Minas, Minas Gerais, Brazil. Anais da
Academia Brasileira de Ciências, 82(2), 293-304.
- Denisow, B., Strzałkowska-Abramek, M., Bożek, M., & Jeżak, A.
(2014). Ornamental Representatives of the Genus
Centaurea L. as a Pollen Source for Bee Friendly Gardens.
Journal of Apicultural Science, 58(2), 49-58. http://doi:
10.2478/jas-2014-0016
- Dimou, M., & Thrasyvoulou, A. (2007). A comparison of three
methods for assessing the relative abundance of pollen
resources collected by honey bee colonies. Journal of
Apicultural Research, 46(3), 143-147.
- Dobson, H. E., & Bergström, G. (2000). The ecology and
evolution of pollen odors. Plant System Evolution, 22(4),
63-87.
- Duncan, D. B. (1955). Multiple range and multiple F tests.
Biometrics, 11(1), 1-42.
- Erdtman, G. (1954). An introduction to pollen analysis.
Chronica Botanica Company, Waltham, Massachusetts,
USA. 239.
- Erdtman, G. (1960). The acetolysis method – a revised
description. Svensk Botanisk Tidskrift, 54, 561–564.
- Goodwin, M. (2012). Pollination of crops in Australia and New
Zealand. The Rural Industries Research and Development
Corporation. pp 121.
- Gosling, W. D., Miller, C. S., & Livingstone, D. A. (2013). Atlas
of the tropical West African pollen flora. Review of
Palaeobotany and Palynology, 199, 1-135.
https://doi.org/10.1016/j.revpalbo.2013.01.003
- Khan, S. U., Zafar, M., Ahmad, M., Anjum, F., Sultana, S., Kilic,
O., Ozdemir, F. A., Nazir, A., Yaseen, G., Aabidin, S. Z., &
Abbas, Q. (2019). Pollen micromorphological analysis of
tribe Acacieae (Mimosaceae) with LM and SEM
techniques. Microscopy Research and Technique, 82(9),
1610-1620. http://doi:10.1002/jemt.23327
- Kirk, W. D. J. (1994). A colour guide to pollen loads of the honey
bee Cardiff: International Bee Research Association, 54.
- Klein, S., Pasquaretta, C., He, X. J., Perry, C., Søvik, E., Devaud,
J. M., & Lihoreau, M. (2019). Honey bees increase their
foraging performance and frequency of pollen trips
through experience. Scientific Reports. 9(1), 1–10.
https://doi.org/10.1038/s41598-019-42677-x.
- Lau, P., Bryant, V., Ellis, J. D., Huang, Z. Y., Sullivan, J., Schmehl,
D. R., Cabrera, A. R., & Rangel, J. (2019). Seasonal variation of pollen collected by honey bees (Apis
mellifera) in developed areas across four regions in the
United States. PLoS One, 14(6). e0217294.
https://doi.org/10.1371/journal.pone.0217294.
- Mărgăoan, R., Özkök, A., Keskin, Ş., Mayda, N., Urcan, A.C., &
Cornea-Cipcigan, M. (2021). Bee collected pollen as a
value-added product rich in bioactive compounds and
unsaturated fatty acids: A comparative study from
Turkey and Romania. LWT, 149, p.111925.
- Mayda, N., Özkök, A., Ecem Bayram, N., Gerçek, Y.C., & Sorkun,
K. (2020). Bee bread and bee pollen of different plant
sources: Determination of phenolic content, antioxidant
activity, fatty acid and element profiles. Journal of Food
Measurement and Characterization, 14, pp.1795-1809.
- Naghiloo, S., & Nikzat Siahkolaee, S. (2019). Does breeding
system affect pollen morphology? A case study in
Zygophylloideae (Zygophyllaceae). Plant Reproduction,
32(4), 381-390. https://doi.org/10.1007/s00497-019-
00379-4.
- Rouhakhsh, H., Davarynejad, G., Abedi, B., & Rahemi, M.
(2014). Pollen grain morphological characteristics of ten
Iranian jujube (Ziziphus jujuba) cultivars by using
scanning electron microscopy. Advances in
Environmental Biology, 1333-1339.
- SAS Institute, (2004). The SAS System (Version 9.1.3). SAS
Institute, Cary, NC.
- Semerdjieva, I., Yankova-Tsvetkova, E., Baldjiev, G., &
Yurukova-Grancharova, P. (2011). Pollen and seed
morphology of Tribulus terrestris L. (Zygophyllaceae).
Biotechnology and Biotechnological Equipment, 25(2),
2379-2382.
- Shawer, M. B., Taha, E. K. A., Mousa, K. M., Khan, K. A., Ibrahim,
S., Hassan, S., & Elnabawy, E. S. M. (2021). Seasonal
variations of colony activities linked to morphometric
and glands characterizations of hybrid Carniolan honey bee (Apis mellifera carnica Pollmann) workers. Journal of
King Saud University-Science, 33(6), 101543.
https://doi.org/10.1016/j.jksus.2021.101543.
- Suryanarayana, M. C., Rao, G. M., & Singh, T. S. M. S. (1992).
Studies on pollen sources for Apis cerana Fabr and Apis
mellifera L. bees at Muzaffarpur, Bihar, India. Apidologie,
23(1), 33-46.
- Taha, E. K. A. (2015). A study on nectar and pollen sources for
honey bee, Apis mellifera L. in Al- Ahsa Saudi Arabia.
Journal of Entomology and Zoology Studies, 3(3), 272-
277.
- Taha, E. K. A., Al-Kahtani, S. N., & Taha, R. (2019). Protein
content and amino acids composition of bee-pollens
from major floral sources in Al-Ahsa, eastern Saudi
Arabia. Saudi Journal of Biological Sciences, 26 (2), 232–
237. https://doi.org/10.1016/j.sjbs.2017.06.003
- Umber, F., Zafar, M., Ullah, R., Bari, A., Khan, M. Y., Ahmad, M.,
& Sultana, S. (2021). Implication of light and scanning
electron microscopy for pollen morphology of selected
taxa of family Asteraceae and Brassicaceae. Microscopy
Research and Technique, 85(1), 373-384.
https://doi.org/10.1002/jemt.23912.
- Yankova-Tsvetkova, E., Semerdjieva, I., Baldjiev, G., &
Yurukova-Grancharova, P. (2011). On the reproductive
biology of Tribulus terrestris L. (Zygophyllaceae):
embryological features; pollen and seed viability.
Biotechnology & Biotechnological Equipment, 25(2),
2383-2387. https://doi.org/10.5504/BBEQ.2011.0030
- Zaitoun, S., Al-Ghzawi, A. A. M., Samarah, N., & Mullen, R.
(2009). Pod production of Prosopis juliflora (Sw.) DC. As
affected by supplementary and honey bee pollination
under arid conditions. Acta Agriculturae Scandinavica
Section B–Soil and Plant Science, 59(4), 349-356.
https://doi.org/10.1080/09064710802227072
Year 2023,
Volume: 15 Issue: 1, 1 - 11, 31.07.2023
Abdulraouf Amro
,
Mohamed Omar Mohamed
Ahmad Al Ghamdi
References
- Abd El Salam, E. H., Ali, M. A., & Ghazala, N. A. (2022).
Evaluation of the Efficiency Different Types of Bee Pollen
Collection Traps in Honey Bee Colonies during Summer
Season. Arab Universities Journal of Agricultural
Sciences, 30(1), 141-146.
http://10.21608/AJS.2022.111909.1448.
- Abou-Shaara, H. F. (2015). Potential honey bee plants of Egypt.
Cerceta˘ri Agronomice în Moldova, 48, 99–108.
https://repository.uaiasi.ro/xmlui/handle/20.500.12811
/1530.
- Abou-Shaara, H. F., Ahmad A. Al-Ghamdi, & Abdelsalam, A. M.
(2013). A suitability map for keeping honey bees under
harsh environmental conditions using Geographical
Information System. World Applied Sciences Journal, 22, 81099-1105. https://doi.org/
10.5829/idosi.wasj.2013.22.08.7384.
- Adgaba, N., Alghamdi, A., Sammoud, R., Shenkute, A., Tadesse,
Y., Ansari, M. J., Sharma, D., & Hepburn, C. (2017).
Determining spatio-temporal distribution of bee forage
species of Al-Baha region based on ground inventorying
supported with GIS applications and Remote Sensed
Satellite Image analysis. Saudi journal of biological
sciences, 24, 1048–1055.
https://doi.org/10.1016/j.sjbs.2017.01.009.
- Agashe, S. N. (2021). Aerobiology for the Clinician: Basic and
Applied Aspects, Pollen Sources, Pollen Calendars. In
Textbook of Allergy for the Clinician, CRC Press, 49-68.
- Al-Ghamdi, A. A. (2007a). Evaluation of various honey bee
foraging activities for identification of potential bee
plants in Riyadh, Saudi Arabia. Annals of Agricultural
Science-Cairo, 52(2), 487.
- Al-Ghamdi, A. A. (2007b). Beekeeping and honey production in
Saudi Arabia. Fifth Conference of Arab Beekeepers
Association. Tripoli, Libya.
- Al-Ghamdi, A. (2009). Comprehensive study for the current
beekeeping industry of imported and native honey bee
in Kingdom of Saudi Arabia. King Abdulaziz City for
Science and Technology, Final report of project, AT-21-
39.
- Al-Ghamdi, A. A., Tadesse, Y., & Adgaba, N. (2020). Evaluation
of major Acacia species in the nursery towards
apicultural landscape restoration around Southwestern
Saudi Arabia. Saudi Journal of Biological Sciences, 27(12),
3385-3389. https://doi.org/10.1016/j.sjbs.2020.09.002.
- Al-Jabr, A. M., & Nour, M. E. (2001). Content of some Saudi
honeys of pollen spectrum and sucrose. Journal of
Agriculture Science, Mansoura University, 26: 4009-
4014.
- Alqarni, A. S. (2020). Differential foraging of indigenous and
exotic honey bee (Apis mellifera L.) races on nectar-rich
flow in a subtropical ecosystem. Insects, 11(4), 254.
https://doi.org/10.3390/insects11040254.
- Alves, R. De França, & Dos Santos De Assis R. F. (2016).
Arecaceae potential for production of monofloral bee
pollen. Grana, 56(4), 294-303.
https://doi.org/10.1080/00173134.2016.1239760.
- Amro, A. M. (2021). Pollinators and pollination effects on three
canola (Brassica napus L.) cultivars: A case study in
Upper Egypt. Journal of King Saud University-Science,
33(1), 101240.
https://doi.org/10.1016/j.jksus.2020.101240.
- Amro, A. M., Omar, M. O., & Ahmed, A. G. (2020). Impact of
using pollen substitutes on performance of honey bee
(Apis mellifera L.) colonies under harsh environmental
conditions. Science and Animal Health, 8(3), 236-256.
https://doi.org/10.15210/sah.v8i3.19992.
- Arien, Y., Dag, A., Yona, S., Tietel, Z., Cohen, T. L., & Shafir, S.
(2020). Effect of diet lipids and omega-6: 3 ratio on
honey bee brood development, adult survival and body
composition. Journal of insect physiology, 124, 104074.
https://doi.org/10.1016/j.jinsphys.2020.104074.
- Asefa, M., Cao, M., He, Y., Mekonnen, E., Song, X., & Yang, J.
(2020). Ethiopian vegetation types, climate and
topography. Plant Diversity, 42(4), 302-311.
https://doi.org/10.1016/j.pld.2020.04.004.
- Begum, H. A., Iqbal, J., & Aziz, A. (2021). Characterization of
pollen profile of Apis mellifera L. in arid region of
Pakistan. Saudi Journal of Biological Sciences, 28(5),
2964-2974. https://doi.org/10.1016/j.sjbs.2021.02.035.
- Belay, A., Solomon, W. K., Bultossa, G., Adgaba, N., & Melaku,
S. (2015). Botanical origin, colour, granulation, and
sensory properties of the Harenna forest honey, Bale,
Ethiopia. Food chemistery, 167, 213-219.
http://dx.doi.org/10.1016/j.foodchem.2014.06.080
- Bilisik, A., Cakmak, I., Saatcioglu, G., Bicakci, A., & Malyer, H.
(2008). Spectrum of pollen collected by honey bees in
bursa lowland area in high season. Uludag Bee Journal,
8(4), 143-148.
https://dergipark.org.tr/en/pub/uluaricilik/issue/53267
/162401
- Boesewinkel, F. D., & Bouman, F. (1984). The Seed: Structure.
In Embryology of Angiosperms, 1ed. Johri, Springer
Berlin Heidelberg, B.,567-610.
- Brodschneider,R.,&Crailsheim,K.(2010). Nutritionandhealthin
honey bees. Apidologie, 41(3), 278-294.
- Collenette, S. (1999). Wild Flowers of Saudi Arabia. National
Commission for Wild Life Conservation and
Development (VCWCD), Riyadh, Saudi Arabia, 799.
- Da luz, C. F. P., Junior, G. L. B., Fonseca, R. L. S. E., & DE Sousa,
P. R. (2010). Comparative pollen preferences by
Africanized honey bees Apis mellifera L. of two colonies
in Pará de Minas, Minas Gerais, Brazil. Anais da
Academia Brasileira de Ciências, 82(2), 293-304.
- Denisow, B., Strzałkowska-Abramek, M., Bożek, M., & Jeżak, A.
(2014). Ornamental Representatives of the Genus
Centaurea L. as a Pollen Source for Bee Friendly Gardens.
Journal of Apicultural Science, 58(2), 49-58. http://doi:
10.2478/jas-2014-0016
- Dimou, M., & Thrasyvoulou, A. (2007). A comparison of three
methods for assessing the relative abundance of pollen
resources collected by honey bee colonies. Journal of
Apicultural Research, 46(3), 143-147.
- Dobson, H. E., & Bergström, G. (2000). The ecology and
evolution of pollen odors. Plant System Evolution, 22(4),
63-87.
- Duncan, D. B. (1955). Multiple range and multiple F tests.
Biometrics, 11(1), 1-42.
- Erdtman, G. (1954). An introduction to pollen analysis.
Chronica Botanica Company, Waltham, Massachusetts,
USA. 239.
- Erdtman, G. (1960). The acetolysis method – a revised
description. Svensk Botanisk Tidskrift, 54, 561–564.
- Goodwin, M. (2012). Pollination of crops in Australia and New
Zealand. The Rural Industries Research and Development
Corporation. pp 121.
- Gosling, W. D., Miller, C. S., & Livingstone, D. A. (2013). Atlas
of the tropical West African pollen flora. Review of
Palaeobotany and Palynology, 199, 1-135.
https://doi.org/10.1016/j.revpalbo.2013.01.003
- Khan, S. U., Zafar, M., Ahmad, M., Anjum, F., Sultana, S., Kilic,
O., Ozdemir, F. A., Nazir, A., Yaseen, G., Aabidin, S. Z., &
Abbas, Q. (2019). Pollen micromorphological analysis of
tribe Acacieae (Mimosaceae) with LM and SEM
techniques. Microscopy Research and Technique, 82(9),
1610-1620. http://doi:10.1002/jemt.23327
- Kirk, W. D. J. (1994). A colour guide to pollen loads of the honey
bee Cardiff: International Bee Research Association, 54.
- Klein, S., Pasquaretta, C., He, X. J., Perry, C., Søvik, E., Devaud,
J. M., & Lihoreau, M. (2019). Honey bees increase their
foraging performance and frequency of pollen trips
through experience. Scientific Reports. 9(1), 1–10.
https://doi.org/10.1038/s41598-019-42677-x.
- Lau, P., Bryant, V., Ellis, J. D., Huang, Z. Y., Sullivan, J., Schmehl,
D. R., Cabrera, A. R., & Rangel, J. (2019). Seasonal variation of pollen collected by honey bees (Apis
mellifera) in developed areas across four regions in the
United States. PLoS One, 14(6). e0217294.
https://doi.org/10.1371/journal.pone.0217294.
- Mărgăoan, R., Özkök, A., Keskin, Ş., Mayda, N., Urcan, A.C., &
Cornea-Cipcigan, M. (2021). Bee collected pollen as a
value-added product rich in bioactive compounds and
unsaturated fatty acids: A comparative study from
Turkey and Romania. LWT, 149, p.111925.
- Mayda, N., Özkök, A., Ecem Bayram, N., Gerçek, Y.C., & Sorkun,
K. (2020). Bee bread and bee pollen of different plant
sources: Determination of phenolic content, antioxidant
activity, fatty acid and element profiles. Journal of Food
Measurement and Characterization, 14, pp.1795-1809.
- Naghiloo, S., & Nikzat Siahkolaee, S. (2019). Does breeding
system affect pollen morphology? A case study in
Zygophylloideae (Zygophyllaceae). Plant Reproduction,
32(4), 381-390. https://doi.org/10.1007/s00497-019-
00379-4.
- Rouhakhsh, H., Davarynejad, G., Abedi, B., & Rahemi, M.
(2014). Pollen grain morphological characteristics of ten
Iranian jujube (Ziziphus jujuba) cultivars by using
scanning electron microscopy. Advances in
Environmental Biology, 1333-1339.
- SAS Institute, (2004). The SAS System (Version 9.1.3). SAS
Institute, Cary, NC.
- Semerdjieva, I., Yankova-Tsvetkova, E., Baldjiev, G., &
Yurukova-Grancharova, P. (2011). Pollen and seed
morphology of Tribulus terrestris L. (Zygophyllaceae).
Biotechnology and Biotechnological Equipment, 25(2),
2379-2382.
- Shawer, M. B., Taha, E. K. A., Mousa, K. M., Khan, K. A., Ibrahim,
S., Hassan, S., & Elnabawy, E. S. M. (2021). Seasonal
variations of colony activities linked to morphometric
and glands characterizations of hybrid Carniolan honey bee (Apis mellifera carnica Pollmann) workers. Journal of
King Saud University-Science, 33(6), 101543.
https://doi.org/10.1016/j.jksus.2021.101543.
- Suryanarayana, M. C., Rao, G. M., & Singh, T. S. M. S. (1992).
Studies on pollen sources for Apis cerana Fabr and Apis
mellifera L. bees at Muzaffarpur, Bihar, India. Apidologie,
23(1), 33-46.
- Taha, E. K. A. (2015). A study on nectar and pollen sources for
honey bee, Apis mellifera L. in Al- Ahsa Saudi Arabia.
Journal of Entomology and Zoology Studies, 3(3), 272-
277.
- Taha, E. K. A., Al-Kahtani, S. N., & Taha, R. (2019). Protein
content and amino acids composition of bee-pollens
from major floral sources in Al-Ahsa, eastern Saudi
Arabia. Saudi Journal of Biological Sciences, 26 (2), 232–
237. https://doi.org/10.1016/j.sjbs.2017.06.003
- Umber, F., Zafar, M., Ullah, R., Bari, A., Khan, M. Y., Ahmad, M.,
& Sultana, S. (2021). Implication of light and scanning
electron microscopy for pollen morphology of selected
taxa of family Asteraceae and Brassicaceae. Microscopy
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