The Use of Some Herbal Essential Oils Against Galleria mellonella Larvae and Testing of Bacillus thuringiensis Bacterium Isolated from Galleria mellonella Under Laboratory Conditions

Year 2024, Volume: 16 Issue: 1, 7 - 13, 31.07.2024

Mehtap Usta

Abstract

Larvae of wax moths cause great damage in honey bee hives and especially in stored honeycombs. Biological control methods are especially important in the control of wax moth in warehouses, as they do not harm the bee, the product and the environment. This study was carried out to determine the effect of 5%, 10%, 25%, 45% and 55% of peppermint, thyme, nettle seed, and walnut herbal oils and GB1 Bacillus sp. (OR227363) against wax moth larvae (Galleria mellonella) (L1-L3) under laboratory conditions. For each group of fifteen larvae, four herbal oil and one bacterial trials were conducted and two control groups were formed. The trials were conducted in glass jars and the larvae were kept in an oven at 25°C temperature/75% relative humidity. Each jar was checked every day for two weeks and dead/viable larvae were recorded and the dead ones were removed from the jar. As a result of the dose trials, it was determined that the best dose was 2.835x109 cfu/mL for bacteria and 5% concentration of thyme and walnut oil for herbal oil. According to the data obtained, it is thought that GB1 Bacillus sp. isolate can be used as an alternative control method against wax moth larvae.

Keywords

Bacteriology, Biological control, Microbiology, Waxmoth

Ethical Statement

Since the study concerns invertebrates does not require any ethics committee authorisation. (Article 4, Paragraph 1-d of the Regulation on the Working Procedures and Principles of Animal Experiments Ethics Committees published in the Official Gazette dated 15/2/2014 and numbered 28914 based on Article 14 of the Higher Education Law No. 2547.)

Thanks

I would like to thank Artvin Çoruh University Beekeeping Research and Application Centre and Filiz Gülbin GÖKDEMİR for their help in the provision and identification of Galleria mellonella.

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