Acrolein-induced histopathological alterations in the liver of goldfish, Carassius auratus (Linnaeus, 1758)

Abstract

The present study was conducted to examine the potential histopathological changes caused by a herbicide, acrolein, in the liver of Carassius auratus (goldfish). Fish were exposed to 1, 5 and 25 µg/L acrolein for 96 h. Liver tissues were removed, fixed with Bouin’s fluid and embedded in paraffin. 5 µm serial sections were stained with hematoxylin eosin and the samples were investigated by light microscopy. Acrolein treatment gave rise to sinusoidal dilatation and congestion, vacuolar degeneration, hemorrhage, lymphocyte infiltration, presence of enlarged melanomacrophagic centers, cloudy swelling, nucleolus absence, and necrosis. These results are important for paying attention to acrolein usage limits and its contamination in the aquatic environment.

Keywords

• Acrolein
• Carassius auratus
• goldfish
• herbicide
• histopathology
• liver

References

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