Histopathology of the Tissue of a Tubificid Worm Limnodrilus hoffmeisteri Exposed to Cadmium

Year 2012, Volume: 9 Issue: 1, - , 01.02.2012

Mediha Canbek , Ahmet Özen Nihal Yerli Mustafa Uyanoğlu Naime Arslan

Abstract

Although cadmium is not an essential element for any organism, oligocheates -
especially Tubificid worms - accumulate large concentrations of cadmium like other freshwater
specimens. In addition, some species such as the ones that belong to Limnodrilus
genera use indicator organisms. The aim of this study is to examine histopathological
alterations induced experimentally by cadmium in tubificid worms (Limnodrilus hoffmeisteri)
exposed for different times, and at different concentrations, and these were compared
to controls. Live specimens were collected at 3 sampling sites from the Porsuk River.
Samples were exposed to the contaminant for short periods of 6, 12, 24, 48 and 72 h at
two different concentrations of 0.25 and 0.5 mg/L. Samples were embedded in paraffin
blocks and were cut at 5 µm on a microtome. All sections were stained using Hematoxylin
& Eosin. Our results showed no alterations (6, 12, 24, 48 h) compared with controls.
However, after exposure for 72 h. results showed different alterations.

Keywords

Heavy metal, cadmium, histopathology, oligochaeta

References

• [1] J. R. Jennings and P. S. Rainbow, Studies on the uptake of cadmium by the crab Carcinus maenas in the laboratory. 1. Accumulation from seawater and a food source, Marine Biology 50 (1979), 131–139.
• [2] R. Najle, M. Elissondo, S. Gentile, M. Gentile, G. Vacarezza and H. Solana, Histopathology of the digestive gland of an Antarctic limpet exposed to cadmium, The Science of the Total Environment 247 (2000), 263–268.
• [3] G. A. Burton, Assessing contaminated aquatic sediments, Environmental Science & Technology 26 (1992), 1862–1863.
• [4] R. Dallinger, F. Prosi, H. Segner and H. Back, Contaminated food and uptake of heavy metals by fish: a review and a proposal for further research, Oecologia 73 (1987), 91–98.
• [5] K. R. Timmermans, E. Spijkerman, M. Tonkes and H. Govers, Cadmium and zinc uptake by two species of aquatic invertebrate predators from dietary and aqueous sources, Canadian Journal of Fisheries and Aquatic Sciences 49 (1992), 655–662.
• [6] A. Z. Mason, The Uptake, Accumulation and Excretion of Metals by the Marine Prosobranch Gastropod Mollusc Littorina Littorea (L.), PhD. Thesis, University of Wales (U.C.N.W., Bangor: Marine Science), 1983.
• [7] I. A. Marig´omez, M. P. Cajaraville and E. Angulo, Histopathology of digestive gland complex of the marine prosobranch Littorina littorea exposed to cadmium, Diseases of Aquatic Organisms 9 (1990), 229–238.
• [8] W. J. G. M. Peijnenburg, R. Baerselman, A. C. de Groot, T. Jager, L. Posthuma and R. P. M. Van Veen, Relating environmental availability to bioavailability: Soil-type-dependent metal accumulation in the oligochaete Eisenia andrei, Ecotoxicology and Environmental Safety 44 (1999), 294–310.
• [9] L. Ramos, M. J. Gonz´alez and L. M. Hern´andez, Sequential extraction of copper, lead, cadmium, and zinc in sediments from Ebro River (Spain): Relationship with levels detected in earthworms, Bulletin of Environmental Contamination and Toxicology 62 (1999), 301–308