Comparison of Microwave, Ultrasonic Bath and Homogenizer Extraction Methods on the Bioactive Molecules Content of Green Tea (Camellia Sinensis) Plant.

Abstract

In this study, the effects of three different extraction methods on the antioxidant capacity, phenolic component, volatile organic molecule and amino acid contents of green tea (Camellia sinensis) leaves were compared. Microwave digestion assisted extraction (MDAE), ultrasonic bath assisted extraction (UBAE) and homogenizer assisted extraction (HAE) methods were used as extraction methods. When the MDAE, UBAE and HAE extraction methods were compared according to the ferric reducing antioxidant power (FRAP) antioxidant activity results, 51.95, 50.9 and 13.8 TE/g dry weight (DW) results were obtained in green tea plant, respectively. DPPH activity in green tea was found to be 94.65, 69.7 and 36.35 TE/g dry weight in microwave extraction, ultrasonic bath and homogenizer extractions, respectively. Analysis in green tea leaves as a result of gas chromatography-mass spectrometry (GC-MS), caffeine, phytol, palmitic acid, beta.-monoglyceride, 13-docosenamide, (z)- and phytol were identified in all three extractions. Fourteen amino acid types were determined in green tea leaves by liquid chromatography-diode array detection (HPLC-DAD). Especially aspargine, glutamine, alanine, serine and L-theanine were determined in very high amounts. When the analysis data were compared, it was determined that the best results were in MDAE>UBAE>HAE, respectively

Keywords

• Extraction
• green tea
• HPLC-DAD
• amino acid
• phenolic compounds
• LC-MS/MS.

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