Determination of phytochemical and antibacterial properties of Momordica charantia seed extracts

Year 2023, , 309 - 313, 29.12.2023

Mehzat Altun , Zerife Orhan

https://doi.org/10.18466/cbayarfbe.1292919

Abstract

In recent years, it is essential to discover safe and effective antibacterial drugs because of rising antibiotic-resistant bacteria. In traditional medicine, plant extracts including biological active components have been used for therapeutic purposes. We aimed to evaluate the antimicrobial properties of the aqueous seed extract of Momordica charantia (M.charantia) on 9 pathogenic bacteria. The antibacterial activity of the extract was assessed against strains using disc diffusion and broth microdilution tests. A total of 21 constituents were identified from M.charantia seeds. Alcohols, esters, aldehydes, monoterpenes, and monoterpenoids were found as the prevalent groups. The seed extract showed the greatest antimicrobial activity on B.subtilis and S.aureus with an inhibition zone diameter (IZD) value of 15.75±0.50 mm and 15.25±0.957 mm, respectively. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) test results ranged from 12.5 to 100 (mg/mL). The seed extract of M.charantia could be used for the cure of bacterial infections as a new natural drug.

Keywords

Momordica charantia, Phytochemical components, Antibacterial activity

References

• [1]. Huemer, M, Mairpady Shambat, S, Brugger, SD, Zinkernagel, AS. 2020. Antibiotic resistance and persistence-Implications for human health and treatment perspectives. EMBO Reports; 21: e51034.
• [2]. Ghosh, D, Veeraraghavan, B, Elangovan, R, Vivekanandan, P. 2020. Antibiotic Resistance and Epigenetics: More to It than Meets the Eye. Antimicrobial agents and chemotherapy; 27:64: e02225-19.
• [3]. Medina, E, Pieper, DH. 2016. Tackling threats and future problems of multidrug-resistant bacteria. How to overcome the antibiotic crisis; Frontiers in Pharmacology; 13:972935.
• [4]. Ali Mirza, S, Afzaal, M, Begum, S, Arooj, T, Almas, M, Ahmed, S, Younus, M. Chapter 11-Uptake Mechanism of Antibiotics in Plants. In: Hashmi M.Z., editor. Antibiotics and Antimicrobial Resistance Genes in the Environment. Volume 1. Elsevier; Amsterdam, The Netherlands, 2020, pp. 183–188.
• [5]. Michelin, DC, Moreschi, PE, Lima, AC, Nascimento, GGF, Paganelli, MO, Chaud, MV. 2005. Avaliação da atividade antimicrobiana de extratos vegetais. Revista Brasileira de Farmacognosia; 15: 316–320.
• [6]. Hussain, MS, Hasan, I, Millat, MS, Sen, N, Rahmad, MA, Rahman, MA, Islam, S, Moghal, MMR. 2018. Ascertainment of pharmacological activities of Allamanda neriifolia Hook and Aegialitis rotundifolia Roxb used in Bangladesh: An in vitro study. Journal of Traditional and Complementary Medicine; 8: 107-112.
• [7]. Awuchi, CG. 2019. Medicinal Plants: the medical, food, nutritional biochemistry, and uses. International Journal of Advanced Academic Research; 5: 220-241.
• [8]. Manandhar, S, Luitel, S, Dahal, RK. 2019. In vitro antimicrobial activity of some medicinal plants against human pathogenic bacteria. Journal of Tropical Medicine; 1895340.
• [9]. Khalid, Z, Hassan, S, Shahzad, S, Khurram, H. 2021. A Review on Biological Attributes of Momordica charantia. Advances in Bioscience, Bioengineering; 9: 8-12.
• [10]. Chen, J, Tian, R, Qiu, M, Lu, L, Zheng, Y, Zhang, Z. 2008. Trinorcucurbitane and cucurbitane triterpenoids from the roots of Momordica charantia. Phytochemistry; 69: 1043–1048.
• [11]. Polito, L, Djemil, A, Bortolotti, M. 2016. Plant Toxin-Based Immunotoxins for Cancer Therapy: A Short Overview. Biomedicines; 4: 12.
• [12]. Grover, JK, Yadav, SP. 2004. Pharmacological actions and potential uses of Momordica charantia: A Review. Journal of Ethnopharmacology; 93: 123–132.
• [13]. Cuong, DM, Kwon, SJ, Jeon, J, Park, YJ, Park, JS, Park, SU. 2018. Identification and characterization of phenylpropanoid biosynthetic genes and their accumulation in bitter melon (Momordica charantia). Molecules; 23: 469.
• [14]. Saeed, MK, Shahzadi, I, Ahmad, I, Ahma, R, Shahzad, K, Ashraf, M, Nisa V. 2010. Nutritional analysis and antioxidant activity of bitter gourd (Momordica charantia) from Pakistan. Pharmacologyonline; 1: 252-260.
• [15]. Kumar, DS, Sharathnath, KV, Yogeswaran, P, Harani, A, Sudhakar, K, Sudha, P, Banji D. 2010. A medicinal potency of Momordica charantia. International Journal of Pharmaceutical Sciences Review and Research; 1: 95-100.
• [16]. Lee, YH, Yoon, SY, Baek, J, Kim, SJ, Yu, JS, Kang, H, Kim, KH. 2021. Metabolite profile of cucurbitane-type triterpenoids of bitter melon (fruit of Momordica charantia) and their inhibitory activity against protein tyrosine phosphatases relevant to insulin resistance. Journal of Agricultural and Food Chemistry;69: 1816–1830.
• [17]. Shimada, T, Kato, F, Dwijayanti, DR, Nagata, T, Kinoshita, A, Okuyama, T, Mukai, E. 2021. Bitter melon fruit extract enhances intracellular ATP production and insulin secretion from rat pancreatic beta cells. British Journal of Nutrition; 127: 377-383.
• [18]. Salinas-Sánchez, DO, Ramírez-Rodríguez, R, Rivas-González, JM. Figueroa-Brito, R, Peña-Chora, G, Toledo-Hernández, E. 2021. Chemical composition of Ricinus communis and Momordica charantia seeds extracts and its bioactivity against the sugarcane aphid, Melanaphis sacchari (Zehntner). International Journal of Tropical Insect Science; 41: 2991–2996.
• [19]. Wang, S, Li, Z, Yang, G, Ho, CT, Li, S. 2017. Momordica charantia: A popular health-promoting vegetable with multi-functionality. Food & Function; 8: 1749-1762.
• [20]. Upadhyay, A, Agrahari, P, Singh, DK. 2015. A review on salient pharmacological features of Momordica charantia. International Journal of Pharmacology; 11: 405-413.
• [21]. Guarniz, W, Canuto, K, Ribeiro, P, Dodou, H, Magalhaes, K, Sa, K. 2019. Momordica charantia L. variety from Northeastern Brazil: Analysis of Antimicrobial Activity and Phytochemical Components. Pharmacognosy Journal; 11: 1312-24.
• [22]. Saini, RK, Keum, YS. 2017. Characterization of nutritionally important phytoconstituents in bitter melon (Momordica charantia L.) fruits by HPLC-DAD and GC-MS. Journal of Food Measurement and Characterization; 11: 119-125.
• [23]. Ghaffar, F, Kainatm, B, Shah, HU, Rahman, IU. 2017. DPPH Radical Scavenging Assay, Biological Activities, Nutricional Composition and Quality Parameters of Momordica charantia Seeds Grown in District CHarsadda, KPK, Pakistan. Pakistan Journal of Scientific and Industrial Research Series B Biological Sciences; 60: 80-86.
• [24]. Braca, A, Siciliano, T, D’Arrigo, M, Germanò, MP. 2008. Chemical composition and antimicrobial activity of Momordica charantia seed essential oil. Fitoterapia; 79: 123-125.
• [25]. Lu, YL, Liu, YH, Chyuan, JH, Cheng, KT, Liang, WL, Hou, WC. 2011. Antibacterial and cytotoxic activities of different wild bitter gourd cultivars (Momordica charantia L. var. abbreviata Seringe). Botanical Studies; 52: 427-434.
• [26]. Leelaprakash, G, Rose, J, Gowtham, B, Javvaji, PK, Prasad, S. 2011. In vitro Antimicrobial and Antioxidant Activity of Momordica charantia Leaves. Pharmacophore; 2: 244-252.
• [27]. Santos, KKA, Matias, EFF, Sobral-Souza, CE, Tintino, SR, Morais-Braga, MB, Guedes, GM, Coutinho, HD. 2012. Trypanocide, cytotoxic, and antifungal activities of Momordica charantia. Pharmaceutical Biology; 50: 162-166.
• [28]. Zhang, F, Lin, L, Xie, JA. 2016. A mini-review of chemical and biological properties of polysaccharides from M. charantia. International journal of biological macromolecules; 92: 246-253.
• [29]. Wu, L, Ke, L, Huang, X, Liu, S, Chen, H, Rao, P. 2006. Separation and characterization of the active ingredients of M. charantia L. and their protective and repairing effect on HIT-T15 cells damaged by alloxan in vitro. Journal of Chinese Institute of Food Science and Technology, 6: 24-28.
• [30]. Makhija, M, Ahuja, D, Nandy, BC, Gautam, S, Tiwari, K, Awasthi, A, Saxena, P. 2011. Evaluation and comparison of antibacterial activity of leaves, seeds, and fruit extract of Momordica charantia. Research Journal of Pharmaceutical, Biological and Chemical Sciences; 2: 185-192.
• [31]. Ibisanmi, TA, Aribisala, JO. 2022. Evaluation of antioxidant, phytochemicals, and antibacterial potential of Mormordica charantia (Linn) against pathogenic bacteria isolated from ready-to-eat food sold in Akure Metropolis, Nigeria. Bulletin of the National Research Centre; 46: 72.
• [32]. Top, R. 2018. Bazı Önemli Tıbbi Bitkilerin Antioksidan, Antimikrobiyal ve Antikanser Etkilerinin Araştırılması. Yüksek Lisans Tezi, Bartın Üniversitesi, Fen Bilimleri Enstitüsü, Biyoloji Anabilim Dalı, Bartın, 62.
• [33]. Muribeca, AJB, Gomes, PWP, Paes, SS, da Costa, APA, Gomes, PWP, Viana, JS, Reis, JDE, Pamplona, SDGSR, Silva, C, Bauermeister, A, Santos, LDS, da Silva, MN. 2022. Antibacterial Activity from Momordica charantia L. Leaves and Flavones Enriched Phase. Pharmaceutics; 14: 1796.
• [34]. Valizadeh, M, Beigomi, M, Fazeli-Nasap, B. 2020. Antibacterial and Antibiofilm Effects of Ethanol and Aceton Leaf Extract of Momordica charantia and Tecomella undulata against Acinetobacter baumannii. International Journal of Advanced Biological and Biomedical Research; 8: 403-418.
• [35]. Ghazanfar, SA. 2007. Plant of Oman. Indigenous Flora; 14-18.