Evaluation of the Genetic Fidelity of in vitro Raised Plants of Origanum majorana L. Using Random Amplified Polymorphic DNA

Abstract

Determination of the genetic characters of the plants obtained using plant tissue culture methods is important. In this study, the genetic fidelity of the plants, which are obtained by the micropropagation of the shoot tip explants of the Origanum majorana L. plant of medicinal and economical value in Murashige and Skoog (MS) medium which contains 1.0 mg/L Benzil Amino Purin (BAP), 0.1 mg/L naphthaleneacetic acid (NAA), 3% sucrose and 0.7% agar, has been investigated by using random amplified polymorphic DNA (RAPD-PCR) technique. Monomorphic bands were obtained as a result of all of the RAPD-PCR analyses performed. According to the results obtained, no polymorphism was detected among the micropropagated plants. 

Keywords

• Genetic fidelity,Medicinal herb,Micropropagation,Origanum majorana L.,RAPD

References

1. 1. Avcı, B, Bayram, E, Geliştirilmiş İzmir Kekiği (Origanum Onites L.) klonlarının farklı ekolojik koşullarda bazı agronomik ve teknolojik özelliklerinin belirlenmesi, Journal of Agriculture Faculty of Ege University, 2013, 50(1), 13-20.
2. 2. Hajlaoui, H, Mighri, H, Aouni, M, Gharsallah, N, Kadri, A, Chemical composition and in vitro evaluation of antioxidant, antimicrobial, cytotoxicity and anti-acetylcholinesterase prop-erties of Tunisian Origanum majorana L. essential oil, Microbial Pathogenesis, 2016, 95, 86-94.
3. 3. Hafez, A. A, Physico-chemical and sensory properties of cakes supplemented with different concentration of marjoram, Australian Journal of Basic and Applied Sciences, 2012, 6(13), 463-470.
4. 4. Tripathy, B, Satyanarayana, S, Khan, K. A, Raja, K, Mohanty, C, Phytochemical screening and antifungal activity of ethanol and petroleum-ether leaf extracts of Origanum majorana, International Journal of Pharmaceutical Research and Health Sciences, 2016, 4(4), 1320-1323.
5. 5. Örçen, N, In vitro organogenesis and regeneration of Alfalfa (Medicago sativa L.) Domestic Cultivar cv. Kayseri. Fresenius Environmental Bulletin, 2013, 22(9a), 2770-2774.
6. 6. Bayraktar, M, Hayta, S, Parlak, S, Gurel, A, Micropropagation of centennial tertiary relict trees of Liquidambar orientalis Miller through meristematic nodules produced by cultures of primordial shoots, Trees, 2015, 29(4), 999-1009.
7. 7. Krishna, H, Alizadeh, M, Singh, D, Singh, U, Chauhan, N, Eftekhari, M, Sadh, R. K, Somaclonal variations and their applications in horticultural crops improvement, 3 Biotech, 2016, 6(54), 2-18.
8. 8. Singh, S.R, Dalal, S, Singh, R, Dhawan, A.K, Kalia, R.K, Evaluation of genetic fidelity of in vitro raised plants of Dendrocalamus asper (Schult. & Schult. F.) Backer ex K. Heyne using DNA-based markers, Acta Physiologiae Plantarum, 2013, 35, 419-430.

9. 9. Cheruvathur, M.K, Thomas, D. T, Shoot organogenesis from root-drived callus of Rhinacanthus nasutus (L) Kurz. and assessment of clonal fidelity of micropropagated plants using RAPD analysis, Applied Biochemistry and Biotechnology, 2014, 172, 1172-1182.
10. 10. Filiz, E, Koç, İ, Bitki biyoteknolojisinde moleküler markörler, Journal of Agriculture Faculty of Gaziosmanpaşa, 2011, 28(2), 207-214.
11. 11. Dam, A, Paul, S, Bhattacharya, C, Bandyopadhyay, T. K, Effects of culture conditions on multiple shoot induction from inflorescence and RAPD analysis of cloned plants in Limonium sinensis (Girard) Kuntze, var. Golden Diamond, Journal of Plant Biochemistry and Biotechnology, 2013, 22(3), 348-352.
12. 12. Rafii, M.Y, Shabanimofrad, M, Edaroyati, M.W.P, Latif, M.A, Analysis of the genetic diversity of physic nut, Jatropha curcas L. accessions using RAPD markers, Molecular Biology Reports, 2012, 39, 6505-6511.
13. 13. Paul, R, Bhau, B.S, Zaman, K, Sharma, H.K, RAPD Analysis of DNA isolated from turmeric rhizomes collected from Northeast India, Advancements in Genetic Engineering, 2016, 5(146), 2169-0111.
14. 14. Martín, C, Kremer, C, González, I, González-Benito, M. E, Influence of the cryopreservation technique, recovery medium and genotype on genetic stability of mint cryopreserved shoot tips. Plant Cell, Tissue and Organ Culture, 2015, 122(1), 185-195.
15. 15. Murashige, T, Skoog, F, A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiologia Plant, 1962, 15, 473-497.
16. 16. Murthy, H. N, Georgiev, M. I, Park, S. Y, Dandin, V. S, Paek, K. Y, The safety assessment of food ingredients derived from plant cell, tissue and organ cultures: a review, 2015, Food chemistry, 176, 426-432.
17. 17. Çetin, B, Demir, C, Duman, M, In 21. National Biology Congress, İzmir, Türkiye, 2012, pp 688-689.
18. 18. Asthana, P, Jaiswal, V. S, Jaiswal, U, Micropropagation of Sapindus trifoliatus L. and assessment of genetic fidelity of micropropagated plants using RAPD analysis, Acta Physiologiae Plantarum, 2011, 33(5), 1821-1829.
19. 19. Chavan, J. J, Gaikwad, N. B, Kshirsagar, P. R, Umdale, S. D, Bhat, K. V, Dixit, G. B, Yadav, S. R, Highly efficient in vitro proliferation and genetic stability analysis of micropropagated Ceropegia evansii by RAPD and ISSR markers: a critically endangered plant of Western Ghats, Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology, 2015, 149(2), 442-450.
20. 20. Joshi, P, Dhawan V, Assesment of genetic fidelity of micropropagared Swertia chirayita plantlets by ISSR marker assay, Biologia Plantarum, 2007, 51(1), 22-26.