Optimization of Cryopreservation Process Using Response Surface Methodology for Chlorella saccharophila and Chlorella zofingiensis

Year 2018, , 405 - 412, 28.12.2018

Zeliha Demirel , Esra Imamoglu , İrem Deniz , Meltem Conk Dalay

https://doi.org/10.18466/cbayarfbe.426444

Abstract

The preservation of microalgae
in a stable state is a fundamental requirement in pharmaceutical, agricultural,
environmental sciences and different industries. Cryopreservation is widely
stabilized for achieving long-term storage and has been applied to an
increasingly diverse range of microalgae and cell cultures. The continuous storage
of actively growing microalgae strains by routine serial subculture is
relatively time-consuming and this technique has possible contamination risks.
In this study, the optimization of cryopreservation process was carried out for
two different Chlorella strains
using response surface methodology (RSM) with three factors (cryoprotectant
concentration, incubation time and cryopreservation time) including 19 runs. The
optimal cell viability of C. zofingiensis
was found at the dimethyl sulfoxide (DMSO) concentration of 12.89% at the incubation
time of 8.14 min and with the cryopreservation time of 93.45 day, while C.
saccharophila was found at the
DMSO concentration of 12.86 % at the incubation time of 7.99 min and at
cryopreservation time of 95.17 day.

Keywords

Cryopreservation, response surface methodology, Chlorella saccharophila, Chlorella zofingiensis, response surface methodology

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