Analytical Method Development and Validation of Phenothrin, Promecarb and Penconazole in Tobacco using Liquid Extraction and Gas Chromatography

Year 2018, , 327 - 331, 30.09.2018

Hale Seçilmiş Canbay

https://doi.org/10.18466/cbayarfbe.446820

Abstract

A sensitive method for the determination of
phenothrin, promecarb and
penconazole residues in tobacco has been developed. Several solvents such as,
hexane, ethyl acetate and dichloromethane were tested in order to minimize
agricultural products residue. In the study, the best recovery was achieved
with dichloromethane. Pesticides residues are extracted from the samples with
dichloromethane. Additional clean-up steps are not required for tobacco
matrices. Determination and quantification of phenothrin, promecarb and penconazole are
performed by gas chromatography-flame ionization detector (GC/FID) and gas
chromatography-nitrogen/phosphorus (GC/NPD). The method has been validated for
tobacco matrices.  Under the optimum
conditions, phenothrin R² value 0.999,   promecarb
R² value 0.999, and penconazole R² value 0.999. Limits of
determination were 0.008 µg mL^-1 for phenothrin, 0.011 µg mL^-1 for
penconazole and 0.007 µg mL^-1 for promecarb. The standard deviations (SDs) levels were in the range
0.01-0.20. Recoveries of phenothrin, promecarb
and penconazole from tobacco were found to be higher than 85%. Under this developed
method, the concentrations of phenothrin, promecarb and penconazole in the 10 tobacco samples were below the
LOD values.

Keywords

Tobacco, GC, phenothrin, promecarb, penconazole, liquid extraction

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