Antimicrobial Potentials of Phyto-synthesized Silver Nanoparticles from Laurus nobilis L.

Abstract

Silver nanoparticles (AgNPs) are popular consumer product additives due to their well-known antimicrobial properties. Nowadays, their usage has increased after the emergence of green synthesis method with less toxicity than conventional methods. In this study, we aimed to reveal the antimicrobial potential of phyto-synthesized AgNP by Laurus nobilis L. extracts against different Gram-positive, Gram-negative bacteria as well as Candida albicans ATCC 10231. Phyto-synthesized AgNPs were characterized by scanning electron microscopy, zeta size-potential analysis, UV-Visible spectroscopy and FT-IR analysis. The results showed that the size of AgNP was 50 nm and coated by phyto-constituents such as phenolic compounds according to FT-IR results. The antimicrobial activity of AgNP was determined by MIC and MBC tests. The results pointed that green synthesized AgNPs are effective against various microorganisms.

Keywords

• Phyto-synthesis,silver nanoparticles,antimicrobial activity,Laurus nobilis,green synthesis

References

1. 1. Abdelghany, T.M., Al-Rajhi, A.M.H., Al Abboud, M.A., Alawlaqi, M.M., Ganash Magdah, A., Helmy, E.A.M. and Mabrouk, A.S. 2017. Recent Advances in Green Synthesis of Silver Nanoparticles and Their Applications: About Future Directions. A Review. BioNanoScience, BioNanoScience, 5–16.
2. 2. Iravani, S. 2011. Green Synthesis of Metal Nanoparticles Using Plants. Green Chemistry, 13, 2638–2650.
3. 3. Dai, L.L. 2012. Advanced Core-Shell Composite Nanoparticles Through Pickering Emulsion Polymerization. The Delivery of Nanoparticles.
4. 4. Korbekandi, H., Iravani, S. and Abbasi, S. 2009. Production of Nanoparticles Using Organisms Production of Nanoparticles Using Organisms. Critical Reviews in Biotechnology, 29, 279–306.
5. 5. Park, Y., Hong, Y.N., Weyers, A., Kim, Y.S. and Linhardt, R.J. 2011. Polysaccharides and Phytochemicals: A Natural Reservoir for the Green Synthesis of Gold and Silver Nanoparticles. IET Nanobiotechnology, 5, 69.
6. 6. Muthu, K. and Priya, S. 2017. Green Synthesis, Characterization and Catalytic Activity of Silver Nanoparticles Using Cassia Auriculata Flower Extract Separated Fraction. Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, Elsevier B.V., 179, 66–72.
7. 7. Chowdhury, N.R., MacGregor-Ramiasa, M., Zilm, P., Majewski, P. and Vasilev, K. 2016. ‘Chocolate’ Silver Nanoparticles: Synthesis, Antibacterial Activity and Cytotoxicity. Journal of Colloid and Interface Science, Elsevier Inc., 482, 151–158.
8. 8. Vijayaraghavan, K. and Ashokkumar, T. 2017. Plant-Mediated Biosynthesis of Metallic Nanoparticles: A Review of Literature, Factors Affecting Synthesis, Characterization Techniques and Applications. Journal of Environmental Chemical Engineering, Elsevier, 5, 4866–4883.

9. 9. Singh, P., Kim, Y.J. and Yang, D.C. 2016. A Strategic Approach for Rapid Synthesis of Gold and Silver Nanoparticles by Panax Ginseng Leaves. Artificial Cells, Nanomedicine and Biotechnology, 44, 1949–1957.
10. 10. Vijayakumar, S., Vaseeharan, B., Malaikozhundan, B. and Shobiya, M. 2016. Laurus Nobilis Leaf Extract Mediated Green Synthesis of ZnO Nanoparticles: Characterization and Biomedical Applications. Biomedicine and Pharmacotherapy, Elsevier Masson SAS, 84, 1213–1222.
11. 11. Ahmad, A., Wei, Y., Syed, F., Tahir, K., Rehman, A.U., Khan, A., Ullah, S. and Yuan, Q. 2017. The Effects of Bacteria-Nanoparticles Interface on the Antibacterial Activity of Green Synthesized Silver Nanoparticles. Microbial Pathogenesis, Elsevier Ltd, 102, 133–142.
12. 12. Clinical and Laboratory Standards Institute (CLSI). 2012. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically ; Approved Standard — Ninth Edition. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standar- Ninth Edition.
13. 13. Molecular, E.K. and Engineering, G. 2014. Green Synthesis of Silver Nanoparticles Using Oak Leaf and Fruit Extracts (Quercus) and Its Antibacterial Activity against Plant Pathogenic Bacteria. International Journal of Biosciences (IJB), 97–103.
14. 14. Siddiqui, M.N., Redhwi, H.H., Achilias, D.S., Kosmidou, E., Vakalopoulou, E. and Ioannidou, M.D. 2018. Green Synthesis of Silver Nanoparticles and Study of Their Antimicrobial Properties. Journal of Polymers and the Environment, Springer US, 26, 423–433.
15. 15. Costa, D., Valente, A.J.M., Queiroz, J.A. and Sousa, Â. 2018. Finding the Ideal Polyethylenimine-Plasmid DNA System for Co-Delivery of Payloads in Cancer Therapy. Colloids and Surfaces B: Biointerfaces, Elsevier, 170, 627–636.
16. 16. Eaton, P., Quaresma, P., Soares, C., Neves, C., de Almeida, M.P., Pereira, E. and West, P. 2017. A Direct Comparison of Experimental Methods to Measure Dimensions of Synthetic Nanoparticles. Ultramicroscopy, Elsevier B.V., 182, 179–190.
17. 17. Ali, Z.A., Yahya, R., Sekaran, S.D. and Puteh, R. 2016. Green Synthesis of Silver Nanoparticles Using Apple Extract and Its Antibacterial Properties. 2016.
18. 18. Ajitha, B., Reddy, Y.A.K., Jeon, H.J. and Ahn, C.W. 2018. Synthesis of Silver Nanoparticles in an Eco-Friendly Way Using Phyllanthus Amarus Leaf Extract: Antimicrobial and Catalytic Activity. Advanced Powder Technology, Society of Powder Technology Japan, 29, 86–93.
19. 19. Rolim, W.R., Pelegrino, M.T., de Araújo Lima, B., Ferraz, L.S., Costa, F.N., Bernardes, J.S., Rodigues, T., Brocchi, M. and Seabra, A.B. 2019. Green Tea Extract Mediated Biogenic Synthesis of Silver Nanoparticles: Characterization, Cytotoxicity Evaluation and Antibacterial Activity. Applied Surface Science, Elsevier, 463, 66–74.
20. 20. Siddiqui, M.N., Redhwi, H.H., Achilias, D.S., Kosmidou, E., Vakalopoulou, E., Ioannidou, M.D., Ahmad, A., Wei, Y., Syed, F., Tahir, K., Rehman, A.U., Khan, A., Ullah, S., Yuan, Q., Molecular, E.K. and Engineering, G. 2018. Green Synthesis of Silver Nanoparticles and Study of Their Antimicrobial Properties. Journal of Polymers and the Environment, Springer US, 26, 423–433.
21. 21. Vijayan, R., Joseph, S. and Mathew, B. 2018. Green Synthesis of Silver Nanoparticles Using Nervalia Zeylanica Leaf Extract and Evaluation of Their Antioxidant, Catalytic, and Antimicrobial Potentials. Particulate Science and Technology, Taylor & Francis, 6351, 1–11.