A Novel Biosensor Based on Laccase for The Detection of Catechol

Abstract

Phenolic compounds are broadly used in petrol industrial production processes and thus they are hazardous for ground and surface water and environment. Many of them have harmful effects on plants, animals and human health. Laccases can oxide phenol and phenolic compounds. Biosensors provide ideal sensing systems to determine phenolic compounds. In the present paper, it was developed novel sensor surface with modified laccase on gold electrode which could recognize the phenolic compound catechol. The electrode arrays integrated microfluidics have been fabricated and characterized in order to create a sensor chip which is easy, rapid, and cheaper to produce also have good electrochemical sensing properties. This gold electrodes were used to obtain laccase active surfaces. Laccase was covalently bounded to self-assembled monolayer on gold electrode via glutaraldehyde reactions. Cyclic voltammetry and amperometry activity assays showed that the detection of catechol with laccase modified gold surface. All of the study was carried out using automated biosensor device. Linear range for this biosensor was 0.025 mM to 0.8 mM.

Keywords

• Catechol,electrochemical biosensor,laccase immobilization

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