Determination of Folic Acid by Ultra-High Performance Liquid Chromatography in Certain Malt-based Beverages after Solid-Phase Extraction

Year 2017, Volume: 13 Issue: 3, 623 - 630, 30.09.2017

Elif Mine Öncü-kaya

https://doi.org/10.18466/cbayarfbe.339315

Cited By: 2

Abstract

The
aim of the present work was to develop and validate an efficient Ultra-High
Performance Liquid Chromatography (UHPLC) method for the determination of folic
acid (FA) in malt-based beverages. Solid-phase extraction (SPE) procedure was
used for cleanup and preconcentration of the malt-based beverages before the
UHPLC analysis. The analysis was performed in a C18 column (2.1x50 mmx1.8 µm) using a
solvent system of ACN: 0.1 % formic acid in water (10:90, v/v) by isocratic
elution. Injection volume was 5 µL. The flow rates of the
mobile phase were maintained at 0.2 mL min^‒1 for 0.00–4.00 min and
0.5 mL min^‒1 for 4.01–12.00 min. Methyl paraben was used as the
internal standard (IS). The FA and IS signals were detected at 284 nm and 254
nm, respectively.
Under these conditions, FA and IS were separated in 3.6 min and 11.4 min,
respectively. The method was successfully validated in terms of precision,
accuracy, linearity, limits of detection (LOD) and quantification (LOQ)
parameters. The relative standard deviations for intra- and inter-day precision
were less than 1.5%. Good linearity with a high correlation coefficient was
achieved over the concentration range of 20.13 µg L⁻¹  – 2004 µg mL⁻¹ for FA. The
LOD and LOQ values were 6.66 µg L⁻¹ and 20.13 µg L⁻¹, respectively. Good recovery values were found ranged between 99.1%
and 106% for boza and vitamin fortified malt drink. The proposed method was successfully applied for the determination of FA
in malt beers, vitamin
fortified malt drinks and boza
samples.

Keywords

Boza, Folic acid, Malt-based beverages, Method development, UHPLC, Validation

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