Determination of Curcumin in Turmeric Sample Using Edge Plane Pyrolytic Graphite Electrode

Year 2017, Volume: 13 Issue: 3, 689 - 694, 30.09.2017

Göksu Basmaz Naciye Öztürk

https://doi.org/10.18466/cbayarfbe.339339

Cited By: 5

Abstract

Phenolic compounds are an important class of the
antioxidants and   found in several natural
products. Research on the phenolic compounds having antioxidant properties present
in natural pruducts like fruits, spices and herbs are increased in recent
years.  Electroanalytical methods have
low detection limits very short analysis time and require less budge as well.
These advantages make the electroanalytical methods favorable and voltammetric analysis
methods are one of the preferred methods in determination of the compounds
having antioxidant properties present in different matrices. In this study, curcumin
in turmeric samples were quantitatively determined by using edge plane
pyrolytic graphite electrode (EPPG) and differential pulse voltammetry.
Electrooxidation behaviour of curcumin was also examined by using cyclic
voltammetry method. A three electrode electrochemical cell was used for
voltammetric analysis. Edge plane pyrolitic graphite (EPPG), was used as working
electrode, saturated calomel electrode (SCE) was used as reference electrode
and Pt wire electrode was used as counter electrode in the cyclic voltamety and
differential pulse voltametry studies. A linear relationship between anodic
peak current and curcumin concentration was observed between 0.325 μM to 1.95μM
at EPPG electrode with differential pulse voltammetry. Detection limit was
calculated as 0,296 μM and the method successfully applied for detection of
curcumin amount in a turmeric sample.

Keywords

Curcumin, Cyclic Voltammetry, Differential Pulse Voltammetry, Edge Plane Pyrolytic Graphite Electrode, Extraction, Turmeric

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